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Keywords:

  • adolescents;
  • preteens;
  • family history;
  • alcohol;
  • body mass index;
  • height growth spurt;
  • peak height growth velocity;
  • age at menarche;
  • benign breast disease;
  • breast cancer

Abstract

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. FUNDING SOURCES
  8. CONFLICT OF INTEREST DISCLOSURES
  9. REFERENCES

BACKGROUND:

Breast cancer (BC) patients wonder how their daughters might reduce their risk. The authors investigated childhood/adolescent risk factors for benign breast disease (BBD), a well-documented risk factor for BC, among girls with a family history.

METHODS:

GUTS (the Growing Up Today Study) includes females, aged 9 to 15 years in 1996, who completed annual questionnaires during 1996 to 2001, then in 2003, 2005, and 2007. Participants provided information regarding alcohol, menarche, height, and body mass index (BMI; kg/m2). Peak height growth velocity (PHV; in./y) was estimated from longitudinal heights. On 2005-2007 surveys, 6888 women (18-27 years old) reported whether they were diagnosed with biopsy-confirmed BBD (n = 67 cases); 6741 women (noncases) reported no BBD. Participants' mothers reported their own biopsy-confirmed BBD and BC, and BC in their sisters and mothers. Stratified by family history, logistic models investigated BBD risk factors.

RESULTS:

Young women whose mothers or aunts had BC were more likely to be diagnosed with BBD (odds ratio [OR], 2.34; P = .01), as were those with maternal BBD (OR, 1.59; P = .095). Adolescents with BC family history (mother, aunt, grandmother) who consumed alcohol (7 drinks/wk) doubled their BBD risk (OR, 2.28; P = .01), similar to those with maternal BBD (OR, 1.96; P = .02). Girls whose mother or aunt had BC saw their BBD risk elevated with higher PHV (OR, 1.82 [inch/yr]; P = .05). Among girls with no family history, BBD risk appeared to be related to other factors: childhood BMI, adolescent waist circumference, and adult height.

CONCLUSIONS:

Adolescents with family history may reduce their risk by avoiding alcohol. Separate risk factors were observed among girls with family history versus girls with no family history, possibly reflecting different causes of BC. Cancer 2011;. © 2011 American Cancer Society.


INTRODUCTION

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. FUNDING SOURCES
  8. CONFLICT OF INTEREST DISCLOSURES
  9. REFERENCES

When family history is a strong risk factor, such as for breast cancer (BC),1-3 advice to patients' family members on lifestyle factors that may further influence their own risk is critically important.4 For BC, this advice is timely for adolescent girls, because the genetic influence is particularly strong at younger ages5; if a woman is diagnosed before age 40 years, her daughter's risk of BC is doubled.6

Certain factors may provide different levels of risk among women with a family history compared with those without a family history.7 In the Nurses' Health Study, for women with a family history of BC, later age at menarche provided little protection against BC, and no reduction in risk was observed with multiple births or with early age at first birth.7 But in both women with and those without a family history of BC, there was increased BC risk associated with their own benign breast disease (BBD).7 In an older Swedish cohort (women aged 50-74 years), BBD and height were related to increased BC risk in subjects without a family history, but not in women with a family history.1 A study of younger BC patients (younger than 50 years), where each control was a patient's own twin sister, found that childhood height and weight were associated with BC risk only among those without a family history.8

BBD is a well-established risk factor for BC.9 Among women with BBD, a family history of BC further increases BC risk.10 And women with a family history of BC are more likely to be diagnosed with BBD, especially at younger ages (25-29 years).11 BBD may itself have a heritable component, with deficient DNA repair genes exerting influence before BBD; the association between BBD and variant alleles in DNA repair genes was significantly stronger among women with a family history of BC.12

The investigation of childhood/adolescent factors and BBD among young women with a family history may provide insight into the etiology of BC or BBD, while providing possible avenues for prevention in those already at higher risk. We recently reported a strong association between adolescent consumption of alcoholic beverages and risk of BBD13; alcohol consumption by adult women is 1 of the few modifiable factors known to increase BC risk.14-16 We also previously reported higher risk for BBD, likely translating into elevated BC risk, among thinner girls, among girls with the most rapid height growth, and among taller young women17; these factors were earlier found to be related to BC risk.18-20 Here we use data from a prospective cohort of children, recruited when they were 9 to 15 years of age, to investigate whether several childhood and adolescent factors are associated with BBD risk in young women with a family history of disease.

MATERIALS AND METHODS

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. FUNDING SOURCES
  8. CONFLICT OF INTEREST DISCLOSURES
  9. REFERENCES

Study Population

Established in 1996, GUTS (the Growing Up Today Study; founding principal investigator: G.A.C.) includes 9037 girls from all 50 states who are daughters of Nurses' Health Study II participants.21 The study, approved by institutional review boards at Harvard School of Public Health and Brigham and Women's Hospital, is described elsewhere.22 Mothers provided informed consent, and their daughters assented by completing baseline questionnaires. The cohort returned follow-up questionnaires annually (on paper or online) from 1996 through 2001, followed by surveys in 2003, 2005, and 2007. The girls' response rate to 1 or more follow-ups after baseline was 97%. A total of 6927 women (77% of cohort) returned the 2005 and/or 2007 (up to January 1, 2009) surveys inquiring about BBD, when follow-up ages were between 18 and 27 years. Of these 6927 women, 6905 responded to the BBD question. From them, we excluded 6 girls because their mothers reported they had been diagnosed with childhood cancer, and another 11 girls whose mothers reported them as adopted; no BBD cases were among these 17 exclusions. This leaves us with a total of 6888 females providing BBD information to these analyses.

Benign Breast Disease

The 2005 and 2007 surveys asked “Has a health care provider ever diagnosed you as having benign breast disease?” and, if yes, whether the diagnosis of BBD had been “confirmed by breast biopsy.” Those 6741 women who responded that they had never been diagnosed with BBD provide the noncases for these analyses. The remaining 147 females reported that they had been given a diagnosis of BBD, although not all were confirmed by biopsy. Among these possible cases are 67 women who reported that their BBD diagnoses were confirmed by breast biopsy, including 27 with biopsy-confirmed BBD reported in both 2005 and 2007, another 29 with confirmed BBD only in 2007 (some returned no 2005 survey), and 11 with confirmed BBD reported in 2005 (but no 2007 survey). These 67 cases and 6741 noncases provided the data for analyses of biopsy-confirmed BBD. (The remaining 80 women [147 − 67] who provided less reliable disease reports were excluded entirely from this work.)

Our questionnaires did not ask for date of diagnosis. Most BBD cases were likely diagnosed because participants (or their physicians) found a clinically palpable mass (which was then biopsied), because participants were too young to be undergoing routine screening mammography. A validation study conducted in a large cohort of women, some of whom are mothers of our participants, confirmed the accuracy of women's self-reports of BBD.23 The most common type of BBD occurring in adolescents and young women (the age group we are studying) are fibroadenomas, which account for nearly 70% of benign lesions.24 The remainder are primarily cysts and fibrocystic changes.24

Risk Factors From Older Childhood and Adolescence

Alcoholic beverage intake

Cumulative alcohol intake (servings/wk of beer, wine, and liquor) was derived from alcohol consumption variables reported on our 2000, 2001, and 2003 surveys; details regarding its derivation were described earlier.13 A review article on the validity of adolescent self-reports of risky behaviors concludes that the privacy of self-administered questionnaires (such as ours) produces higher, supposedly more valid, reported rates of alcohol use.25

Height, weight, and adiposity

Children reported their heights and weights on every survey, and relative weight status was represented by body mass index (BMI = weight/height2, [kg/m2]). Young adult BMI and height were assessed from the 2005 and 2007 surveys, when participants were between the ages of 18 and 27 years. Further details regarding each of these factors and their validity were provided earlier.17 We also computed change in BMI for each girl, from childhood to young adulthood. The participants, as part of the year 2000 survey when they were 13 to 19 years of age, reported their adolescent waist circumference in inches, using a tape measure included with the survey mailing.

Peak height growth velocity

Earlier studies of peak height velocity (PHV) and its dietary correlates26, 27 were conducted because of the belief that PHV may be related to adult diseases. From the serial heights on a girl, we computed a series of annualized height growth increments, HTt − HTt−1, divided by the time intervals (in years, to the month) between adjacent survey return dates. For each girl who was premenarchal at baseline, we inspected her series of annualized growth increments and designated the largest her PHV (in./y). Further details were provided earlier.17 The subgroup of girls who were premenarchal at baseline provided estimates of PHV for 34 BBD cases and 3848 noncases.

Age at menarche

Our surveys annually asked the girls, “Have you started having menstrual periods?” and “If yes, age when periods began.”

Family history

Our participants' mothers provided information regarding their own diagnoses of biopsy-confirmed BBD (to the year 2005) and BC (to 2009), and BC in their mothers and sisters (to 2005). Their mothers and sisters are the maternal grandmothers and aunts of our participants.

Other variables

At baseline, participants reported their race/ethnic group by marking all (of 6) options that applied to them. Most females in this cohort are white/non-Hispanic (95%), as are all but 3 of the 67 BBD cases. We computed all ages (to the month) from dates of questionnaire return and birth.

Statistical Analyses

We estimated the prevalence of family history of BC and maternal BBD for our GUTS participants. The prevalence of biopsy-confirmed BBD in our participants was estimated as well. Because we did not have information regarding when GUTS BBD cases were diagnosed, the outcome for analyses was prevalent BBD in logistic regression models. These models were estimated using SAS,28 which provided odds ratios (ORs) and 95% confidence intervals (CIs) for each risk factor, and the Hosmer-Lemeshow test of goodness of model fit. We used exact logistic regression whenever the number of BBD cases, in stratified analyses, was <10. Because age was related to each female's chance of being diagnosed with BBD during follow-up, we adjusted all models for exact age (to the month) at baseline; earlier work supported this particular age adjustment.17 Our first series of models investigated how family history impacts risk for BBD. Subsequent models tested hypotheses, among girls with a family history, that childhood and adolescent body fatness, PHV, age at menarche, young adult (at 18 years or older) BMI and height, and adolescent alcohol intake are associated with BBD risk.

RESULTS

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. FUNDING SOURCES
  8. CONFLICT OF INTEREST DISCLOSURES
  9. REFERENCES

Prevalence of Family History, Selection Bias

Seventy-seven percent of females in the baseline (1996) GUTS cohort returned the 2005 and/or 2007 surveys that contained our questions regarding BBD. Of the original baseline cohort, 3.83% had a mother with BC; there was little difference in this percentage between females included and absent (because of no 2005 or 2007 survey) from the present analyses (3.85% vs 3.75%; P = .86). More than 18% of the girls' mothers had a biopsy-confirmed BBD, again with little difference between those present versus those absent from these analyses (18.7% vs 18.6%; P = .81). Maternal grandmothers with BC were similarly represented (11.0% vs 10.8%; P = .73), as were girls with an aunt (mother's sister) with BC (3.5% vs 3.0%; P = .26). Our earlier papers13, 17 assessed selection bias in the other risk factors investigated here; included girls tended to be slightly younger at baseline (by 6 weeks), and reported slightly less alcohol consumption (by 0.01 drink/week) (both P < .05), but they were similar at baseline (to those absent from these analyses) in age-adjusted BMI, height, menarche status, and total energy intake.

Impact of Family History on BBD Prevalence

The prevalence of BBD ranged from 0.75% for girls with no family history (no maternal BBD and no family history of BC) to 1.9% and 2.5% for girls whose mothers or aunts had BC (Table 1). Means, within family history groups, of the factors to be investigated are presented in Table 1. Age-adjusted logistic models (Table 2) indicated that maternal BBD may increase a daughter's risk of BBD by >50% (marginally significant, P = .096), whereas BC in the mother or aunt doubled her risk of BBD (OR, 2.34; P = .01), but BC in the grandmother did not increase the granddaughter's risk (OR, 1.41; P = .32). It was unexpected that an aunt with BC (OR, 2.71; 95% CI, 1.16-6.34) appeared a stronger risk factor than a mother with BC (OR, 2.07; 95% CI, 0.83-5.20), but this difference was not statistically significant. The mean age at BC diagnosis among these mothers was 47.6 years; mean diagnosis age among the aunts was 47.4 years, and it was 60.9 years among the grandmothers. Thus, BBD cases in our GUTS females (in their 20s) appear to be more strongly linked to BC cases diagnosed at younger ages in their mothers and aunts, than to cases in their grandmothers, many of them diagnosed at much older ages. Relative to girls with no family history of BC or maternal BBD, girls whose mother or aunt was diagnosed with BC before age 50 years were at greater BBD risk (OR, 2.88; P = .01) than girls whose mother or aunt were diagnosed at age 50 years or later (OR, 2.67; P = .11). Similarly, girls whose mother, aunt, or grandmother had BC diagnosed before age 50 years again had greater BBD risk (OR, 2.35; P = .03) than girls whose family member was diagnosed later (OR, 2.06; P = .04). Girls with a family history of BC (mother, aunt, or grandmother) were at significantly increased risk for BBD (OR, 1.92; 95% CI, 1.12-3.27), as were those with a family history of BC or maternal BBD (OR, 1.97; 95% CI, 1.22-3.20). Furthermore fitting a dose-response model (number of family members, counting multiple aunts, with BC) showed that girls with 1 family member may be at increased risk for BBD (OR, 1.74; P = .058); those with 2 or more family members were at considerably greater risk (OR, 4.26; P = .02).

Table 1. Characteristics of GUTS Girls by Family History of BC or Maternal BBD
 No Family HistoryaMother BBDMother BCAunt BCGrandmother BC
  • Abbreviations: BBD, benign breast disease; BC, breast cancer; BMI, body mass index; PHV, peak height velocity.

  • GUTS (Growing Up Today Study) girls: n=67 biopsy-confirmed BBD cases, 6741 noncases.

  • a

    This group omits girls with either maternal BBD or family history of BC.

GUTS girls, No.46781264260237749
GUTS BBD cases (biopsy-confirmed)35185610
BBD cases, %0.751.421.922.531.34
Risk factor means     
 Baseline age, y12.012.112.012.112.1
 Adolescent alcohol, drinks/d0.20.20.20.20.2
 PHV, in./y3.43.33.33.63.3
 Menarche age, y12.812.912.812.912.9
 Adult height, in.65.465.365.365.265.4
 Childhood BMI, kg/m218.217.918.418.118.1
 BMI change, kg/m24.54.54.54.24.3
 Young adult BMI, kg/m223.723.523.923.323.5
 Adolescent waist circumference, in.29.529.229.529.329.5
Table 2. Risk of Biopsy-Confirmed BBD in GUTS Girls (n=67 Cases, 6741 Noncases) Associated With Family History of BC or Maternal BBD
 UnivariateMultivariate: Family BC, Mother BBDMultivariate: Family BC
ORPORPORP
  • Abbreviations: BBD, benign breast disease; BC, breast cancer; OR, odds ratio.

  • ORs were obtained from logistic regression models; all models include baseline ages of GUTS (Growing Up Today Study) girls.

  • a

    Mother and aunt BC cases combined.

Mother BC2.07.121.85.1951.91.17
Maternal aunt BC2.71.0222.52.032.57.03
Maternal grandmother BC1.41.3241.33.411.36.38
Mother BBD1.59.0951.52.13  
Mother and/or aunt BCa2.34.012.24.022.32.015

Risk Factors for BBD, in Girls With Family History of Breast Cancer

Because of our small number of biopsy-confirmed BBD cases in GUTS females, we recommend cautious interpretation of findings from logistic models fit to subgroups defined by family history (Table 3). The effect of each risk factor was estimated from a separate age-adjusted model. Girls whose mothers had BC may be at even greater risk if they regularly drink alcohol (OR, 4.03 for those who consume 7 drinks/wk compared with nondrinkers; 95% CI, 0.65-16.71; P = .11); the risk is similar for girls whose aunt had BC (OR, 3.60 for those consuming 7 drinks/wk; 95% CI, 0.81-16.10; P = .09). (For interpreting our results, “7 drinks per week” is equivalent to “per daily drink.”) The combined (mother or aunt) risk (OR, 3.80; P = .02) is shown in Table 3, along with the alcohol risk for girls whose grandmother had BC (OR, 2.29; 95% CI, 1.06-4.95; P = .04). The small number of GUTS girls with BBD (5 whose mother had BC, 6 whose aunt had BC, and 10 whose grandmother had BC) represents 19 different girls diagnosed with BBD, so there is little overlap in cases. It is therefore impressive that alcohol has a large OR in separate analyses of the 3 groups (mother OR, 4.03; aunt OR, 3.60; grandmother OR, 2.29). For 1157 girls with any family history of BC (mother, aunt, grandmother), the BBD risk for those who consume 7 alcoholic drinks per week, compared with nondrinkers, is OR 2.28 (P = .01; Table 3). Among girls whose mothers had BC, the only other factor that appeared to increase risk was more rapid PHV (OR, 2.03 [in./y]; 95% CI, 0.90-4.55; P = .08); for girls whose aunt had BC, the estimated risk associated with PHV was OR 1.90 (in./y; 95% CI, 0.84-4.03; P = .11). For mothers and aunts combined, the estimated effect was OR 1.82 (in./y; P = .05; Table 3), but this effect was entirely absent for girls whose grandmothers had BC (OR, 0.71; P = .51; Table 3). None of the other factors appeared important for girls with a family history of BC.

Table 3. Risk of Biopsy-Confirmed BBD in Young Females With Family History of BC, Family History of Maternal BBD, and No Family History
 BC in Affected Family MemberBDD in MotherNo Family History
Mother or AuntGrandmotherAny Family Member (Mother, Aunt, Grandmother)
  1. Abbreviations: BBD, benign breast disease; BC, breast cancer; BMI, body mass index; GUTS, Growing Up Today Study; OR, odds ratio; PHV, peak height velocity.

  2. BMI change is from baseline to young adulthood. Childhood BMI and BMI change appear together in models. ORs were obtained from age-adjusted logistic regression models.

GUTS girls, No.477749115712644678
GUTS BBD cases, No.1010191835
Risk factor, OR (P)     
 Adolescent alcohol, daily drink3.80 (.02)2.29 (.04)2.28 (.01)1.96 (.02)1.22 (.57)
 PHV, in./y1.82 (.05)0.71 (.51)1.21 (.49)1.31 (.44)1.08 (.70)
 Menarche age, y1.21 (.47)1.08 (.77)1.05 (.78)1.00 (.99)1.12 (.42)
 Young adult height, in.0.95 (.67)0.93 (.54)0.96 (.64)1.07 (.44)1.11 (.08)
 Childhood BMI, kg/m21.00 (.97)0.83 (.16)0.93 (.37)0.99 (.90)0.88 (.055)
 BMI change, kg/m21.03 (.72)1.06 (.59)1.04 (.58)1.05 (.44)0.94 (.33)
 Young adult BMI, kg/m21.02 (.81)0.94 (.51)0.99 (.80)1.02 (.63)0.91 (.06)
 Adolescent waist circumference, in.0.92 (.51)0.90 (.37)0.91 (.27)1.08 (.30)0.86 (.02)

Risk Factors for BBD, in Girls With Maternal BBD

Among 1264 girls whose mothers had BBD, 18 of these girls were themselves biopsy-confirmed BBD cases (Table 3). Girls with maternal BBD were at higher risk for BBD the more alcohol they consumed (OR, 1.96 for 7 drinks per week; P = .02). None of the other factors was important.

Combined Risks for BBD: Alcohol

Above we found that girls with a family history of BC were at increased risk for BBD the more alcohol they consumed (OR, 2.28 [daily drink]; P = .01; Table 3). We similarly found that girls with maternal BBD were at increased risk if they drink (OR, 1.96 [daily drink]; P = .02; Table 3). A further analysis (not shown) finds the combined risk of OR 2.02 (daily drink; P = .004) for girls with either maternal BBD or a family history of BC, whereas the estimated risk for girls with both maternal BBD and familial BC is OR 2.36 (daily drink; P = .08). For girls with maternal BBD but no family history of BC, the estimated risk associated with alcohol is OR 1.75 (daily drink; P = .12), and for girls with any family history of BC but no maternal BBD the estimate is OR 2.24 (daily drink; P = .066). Finally, looking at the combined risk associated with family history and alcohol, girls with any family history of disease (BC or maternal BBD) and who are in the highest quartile of alcohol consumption for their age (≥1 drink/wk for age 16 years, 2 drinks/wk for 18 years, 3 drinks/wk for 19 years) have significantly greater BBD risk (OR, 2.27; P = .03) relative to girls with no family history who do not drink any alcohol.

Risk Factors for BBD in Girls With No Family History of BC and No Maternal BBD

We now briefly look at girls with no family history of BC and no maternal BBD (n = 4678); 35 of these girls were BBD cases (Table 3, far right column). The important risk factors for these participants differed from the risk factors for those with a family history of disease. Elevated BBD risk was marginally associated with adult height (OR, 1.11 [inches]; P = .08), childhood BMI (OR, 0.88 [kg/m2]; P = .055), and young adult BMI (OR, 0.91 [kg/m2]; P = .06) and significantly associated with adolescent waist circumference (OR, 0.86 [inches]; P = .02). The other factors were null.

DISCUSSION

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. FUNDING SOURCES
  8. CONFLICT OF INTEREST DISCLOSURES
  9. REFERENCES

Women who have been diagnosed with BC, or whose mother or sister have had the disease, may ask how their daughters might reduce their cancer risk. Our analyses of young women consistently suggested, regardless of the exact nature of family history (BC in her mother, aunt, or grandmother, or biopsy-confirmed BBD in her mother), that avoiding alcohol intake during adolescence may reduce her risk of BBD as a young woman, which likely reflects reduced risk of BC.9 This is consistent with retrospective studies that linked adolescent alcohol intake to BC.18, 29 Our family history subgroups had little overlap and thus provided fairly independent analyses, yet each produced a similar conclusion regarding alcohol consumption by adolescent females. Other factors that often are associated with BC risk (less adiposity from childhood to young adulthood, and greater adult height) were associated with BBD only among girls with no family history of BC or maternal BBD. Age at menarche was null for all family history groups; this was not unexpected, because women with BBD are not protected against BC by later menarche.30 Our finding that more rapid PHV may increase BBD risk among girls whose mother or aunt had BC is consistent with a heritable component in which deficient DNA repair genes exert influence before BBD.12 Earlier research suggested that more rapid height growth is associated with elevated risk of BC.18-20 However, we have less confidence in our finding on rapid height growth and BBD, because the estimated effects were less consistent across family history subgroups, particularly for grandmothers with BC.

Our finding that females with a family history of BC are more likely to themselves be diagnosed with BBD (OR, 1.92; 95% CI, 1.12-3.27) is consistent with an earlier study in which women aged 25 to 29 years with a family history of BC were at comparable elevated risk (relative risk [RR], 1.96; 95% CI, 1.55-2.47).11 One possible explanation for BC in aunts providing slightly higher risk than BC in mothers (Table 2), although not statistically significant and probably because of chance, is that if an aunt had BC and did not survive, that aunt's cancer was reported to us by the participant's mother, but if the mother had BC and did not survive to 1996, when our study was initiated by contacting the mothers, then that daughter never became part of the GUTS cohort. It was similarly unexpected that grandmothers' BC was not a stronger risk factor, but many BC cases in grandmothers occurred at older ages, whereas BBD in GUTS girls (now in their 20s) may be a risk factor for BC at younger ages (as in their mothers and aunts). This is consistent with a study11 in which women with a first-degree relative with BC were at even greater risk for BBD if the relative was diagnosed before age 50 years (RR, 1.81) than after age 50 years (RR, 1.57), although our grandmothers were second-degree relatives. That study further demonstrated that adult women with a family history of BC are at especially increased risk for the high-risk types of BBD (proliferative changes with atypia) that are more strongly associated with BC.

Our analyses support the concept that risk factors for BC may differ between women with a family history of BC and women without a family history.7 Our finding that adult height appeared to be associated with BBD risk only among girls without a family history (Table 3) is consistent with data from a Swedish cohort (women aged 50-74 years), in which height was related to increased BC risk in subjects without a family history, but not in subjects with a family history.1 A study of young BC patients (under 50 years of age) whose controls were their own twin sisters found that childhood height and weight were associated with BC risk only among those without a family history,8 again consistent with our findings.

The longitudinal design is a major strength of this investigation, as alcoholic beverage intakes, height, weight, and menarche data were collected years before the collection of BBD data in this large cohort of young females from across the United States. We controlled for baseline age in all models, but some residual and unmeasured confounding may remain; multivariate analyses in our earlier publications13, 17 provided ORs that were barely different from the age-adjusted estimates (except for young adult BMI, whose effect was greatly diminished with childhood BMI in the model).

A major limitation is the small number of GUTS BBD cases within family history subgroups, particularly girls having mothers with BC and girls having aunts with BC. Logistic model estimates obtained from data including <10 outcome events (BBD cases) should be interpreted with great caution, as there may be a 10% bias away from the null in the estimated effect of any continuous risk factor.31 But the general consistency of our estimated alcohol effects, across family history subgroups, enhances confidence in those conclusions. Another limitation was the necessity to collect data by self-report on (paper and online) questionnaires, but with our large, geographically dispersed cohort, alternatives were not feasible. We cited a validation study demonstrating that young women who reported BBD confirmed by biopsy were very reliable.23 Another issue regards detection bias, for girls with a family history of disease are much more likely to seek medical attention for a lump, and their physicians are more likely to perform a biopsy.32 This may result in more valid outcome data for girls with (than without) a family history, which is not a major problem for this particular analysis, because our primary purpose is to study those with a family history. But among GUTS girls without any known family history, this may indicate underdiagnosis of BBD. Thus, adolescent alcohol consumption may still be an important BBD risk factor for those without any family history, but we may have underestimated the alcohol effect because of the larger number of girls whose BBD is undiagnosed. However, our findings regarding the other factors (height and adiposity) among those with no family history are generally consistent with published risk factors for BC. Reporting errors in childhood height, weight, menarche, and alcohol consumption are likely nondifferential with respect to BBD status later on, resulting in underestimates of true associations.

Another possible limitation is that, although this was intended to be a cohort of biological offspring (we sent recruitment letters only to women with 1 or more childbirths during the relevant time period), a small number of adopted children apparently were entered into the cohort by the mothers along with their biological children. We already excluded entirely (from all analyses in this paper) those daughters who the mothers reported (after baseline) as adopted. However, we further conducted sensitivity analyses in which we excluded an additional small number of girls who we suspect may be adopted (for example, if the biological father's height and maternal weight gain during pregnancy was not reported by the mother), and replicating our analyses produced ORs nearly identical to those reported here.

Although our cohort is not representative of US females, the comparison of risks within our cohort should still be valid and generalizable.33 Because our participants are daughters of nurses, this reduces confounding by socioeconomic and other unmeasured factors, while enhancing the accuracy of the information provided. But the racial/ethnic makeup of our cohort (95% white/non-Hispanic) hinders generalization to other races and ethnicities. Continued follow-up of this cohort will increase both the number of young women diagnosed with BBD and the number with a family history of disease; future work should evaluate risk factors separately for females with and without a family history.

In conclusion, alcohol consumption by adult women is among the few modifiable factors known to increase BC risk,14-16 and our work provides evidence that girls with a family history may reduce their own risk by avoiding alcohol intake during adolescence.

FUNDING SOURCES

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. FUNDING SOURCES
  8. CONFLICT OF INTEREST DISCLOSURES
  9. REFERENCES

Grant DK46834 (principal investigator [PI], G.A.C.) from the National Institutes of Health (Bethesda, Md) funded the initiation of the cohort and the collection/management of the data during early years of follow-up. The Breast Cancer Research foundation (New York, New York) funded the design, conduct, and analysis of this specific article (PI, G.A.C.). Neither sponsor was involved in the preparation, review, or approval of the article. A.L.F. was supported by an award from the American Institute for Cancer Research. G.A.C. was supported in part by an American Cancer Society Clinical Research Professorship.

REFERENCES

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. FUNDING SOURCES
  8. CONFLICT OF INTEREST DISCLOSURES
  9. REFERENCES