The first 3 authors contributed equally to this article.
Copy number alterations of c-MYC and PTEN are prognostic factors for relapse after prostate cancer radiotherapy
Article first published online: 26 JAN 2012
Copyright © 2012 American Cancer Society
Volume 118, Issue 16, pages 4053–4062, 15 August 2012
How to Cite
Zafarana, G., Ishkanian, A. S., Malloff, C. A., Locke, J. A., Sykes, J., Thoms, J., Lam, W. L., Squire, J. A., Yoshimoto, M., Ramnarine, V. R., Meng, A., Ahmed, O., Jurisca, I., Milosevic, M., Pintilie, M., van der Kwast, T. and Bristow, R. G. (2012), Copy number alterations of c-MYC and PTEN are prognostic factors for relapse after prostate cancer radiotherapy. Cancer, 118: 4053–4062. doi: 10.1002/cncr.26729
- Issue published online: 3 AUG 2012
- Article first published online: 26 JAN 2012
- Manuscript Accepted: 1 NOV 2011
- Manuscript Revised: 24 OCT 2011
- Manuscript Received: 20 AUG 2011
Erratum: Erratum: Zafarana G, Ishkanian AS, Malloff CA, Locke JA, Sykes J, Thoms J, Lam WL, Squire JA, Yoshimoto M, Ramnarine VR, Meng A, Ahmed O, Jurisca I, Milosevic M, Pintilie M, van der Kwast T and Bristow RG. Copy number alterations of c-MYC and PTEN are prognostic factors for relapse after prostate cancer radiotherapy. Cancer. 2012;118:4053-62.
Vol. 120, Issue 15, 2380, Article first published online: 18 APR 2014
- prostate cancer;
- genetic instability;
- comparative genomic hybridization;
- Gleason score;
- prostate-specific antigen;
- T category
Despite the use of PSA, Gleason score, and T-category as prognosticators in intermediate-risk prostate cancer, 20–40% of patients will fail local therapy. In order to optimize treatment approaches for intermediate-risk patients, additional genetic prognosticators are needed. Previous reports using array comparative genomic hybridization (aCGH) in radical prostatectomy cohorts suggested a combination of allelic loss of the PTEN gene on 10q and allelic gain of the c-MYC gene on 8q were associated with metastatic disease. We tested whether copy number alterations (CNAs) in PTEN (allelic loss) and c-MYC (allelic gain) were associated with biochemical relapse following modern-era, image-guided radiotherapy (mean dose 76.4 Gy). We used aCGH analyses validated by fluorescence in-situ hybridization (FISH) of DNA was derived from frozen, pre-treatment biopsies in 126 intermediate-risk prostate cancer patients. Patients whose tumors had CNAs in both PTEN and c-MYC had significantly increased genetic instability (percent genome alteration; PGA) compared to tumors with normal PTEN and c-MYC status (p < 0.0001). We demonstrate that c-MYC gain alone, or combined c-MYC gain and PTEN loss, were increasingly prognostic for relapse on multivariable analyses (hazard ratios (HR) of 2.58/p = 0.005 and 3.21/p = 0.0004; respectively). Triaging patients by the use of CNAs within pre-treatment biopsies may allow for better use of systemic therapies to target sub-clinical metastases or locally recurrent disease and improve clinical outcomes. Cancer 2012. © 2012 American Cancer Society.