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Intraoperative detection and removal of microscopic residual sarcoma using wide-field imaging†
Article first published online: 21 MAR 2012
Copyright © 2012 American Cancer Society
Volume 118, Issue 21, pages 5320–5330, 1 November 2012
How to Cite
Mito, J. K., Ferrer, J. M., Brigman, B. E., Lee, C.-L., Dodd, R. D., Eward, W. C., Marshall, L. F., Cuneo, K. C., Carter, J. E., Ramasunder, S., Kim, Y., Lee, W. D., Griffith, L. G., Bawendi, M. G. and Kirsch, D. G. (2012), Intraoperative detection and removal of microscopic residual sarcoma using wide-field imaging. Cancer, 118: 5320–5330. doi: 10.1002/cncr.27458
- Issue published online: 19 OCT 2012
- Article first published online: 21 MAR 2012
- Manuscript Accepted: 17 JAN 2012
- Manuscript Received: 5 DEC 2011
- Manuscript Revised: 5 DEC 2011
- Unknown funding agency
- optical molecular imaging;
- intraoperative imaging;
- soft tissue sarcoma;
- cathepsin proteases
The goal of limb-sparing surgery for a soft tissue sarcoma of the extremity is to remove all malignant cells while preserving limb function. After initial surgery, microscopic residual disease in the tumor bed will cause a local recurrence in approximately 33% of patients with sarcoma. To help identify these patients, the authors developed an in vivo imaging system to investigate the suitability of molecular imaging for intraoperative visualization.
A primary mouse model of soft tissue sarcoma and a wide field-of-view imaging device were used to investigate a series of exogenously administered, near-infrared (NIR) fluorescent probes activated by cathepsin proteases for real-time intraoperative imaging.
The authors demonstrated that exogenously administered cathepsin-activated probes can be used for image-guided surgery to identify microscopic residual NIR fluorescence in the tumor beds of mice. The presence of residual NIR fluorescence was correlated with microscopic residual sarcoma and local recurrence. The removal of residual NIR fluorescence improved local control.
The authors concluded that their technique has the potential to be used for intraoperative image-guided surgery to identify microscopic residual disease in patients with cancer. Cancer 2012. © 2012 American Cancer Society.