• Chernobyl;
  • papillary thyroid carcinoma;
  • iodine-131;
  • RET/PTC;
  • PAX8/PPARγ


  1. Top of page
  2. Abstract


Childhood exposure to iodine-131 from the 1986 nuclear accident in Chernobyl, Ukraine, led to a sharp increase in papillary thyroid carcinoma (PTC) incidence in regions surrounding the reactor. Data concerning the association between genetic mutations in PTCs and individual radiation doses are limited.


Mutational analysis was performed on 62 PTCs diagnosed in a Ukrainian cohort of patients who were < 18 years old in 1986 and received 0.008 to 8.6 Gy of 131I to the thyroid. Associations between mutation types and 131I dose and other characteristics were explored.


RET/PTC (ret proto-oncogene/papillary thyroid carcinoma) rearrangements were most common (35%), followed by BRAF (15%) and RAS (8%) point mutations. Two tumors carrying PAX8/PPARγ (paired box 8/peroxisome proliferator-activated receptor gamma) rearrangement were identified. A significant negative association with 131I dose for BRAF and RAS point mutations and a significant concave association with 131I dose, with an inflection point at 1.6 Gy and odds ratio of 2.1, based on a linear-quadratic model for RET/PTC and PAX8/PPARγ rearrangements were found. The trends with dose were significantly different between tumors with point mutations and rearrangements. Compared with point mutations, rearrangements were associated with residence in the relatively iodine-deficient Zhytomyr region, younger age at exposure or surgery, and male sex.


These results provide the first demonstration of PAX8/PPARγ rearrangements in post-Chernobyl tumors and show different associations for point mutations and chromosomal rearrangements with 131I dose and other factors. These data support the relationship between chromosomal rearrangements, but not point mutations, and 131I exposure and point to a possible role of iodine deficiency in generation of RET/PTC rearrangements in these patients. Cancer 2013. © 2013 American Cancer Society.


  1. Top of page
  2. Abstract

Exposure to ionizing radiation during childhood is known to cause thyroid cancer, with a significantly dose-dependent increased incidence, particularly in children and young adults.1 After the nuclear accident in April 1986 in Chernobyl, Ukraine, residents of regions surrounding the Chernobyl nuclear power plant, including Ukraine, Belarus, and the Russian Federation, received variable doses of radioiodines through inhalation and ingestion of contaminated dairy products or vegetables. These regions experienced a dramatic rise in incidence of thyroid cancer,2 with at least 5000 new cases observed in individuals exposed during childhood or adolescence.3 Papillary thyroid carcinoma (PTC) is known to be the principal type of thyroid carcinoma associated with radiation exposure and comprised the majority of pediatric thyroid tumors in residents of the regions surrounding Chernobyl.2, 4, 5

Case-control and cohort studies of post-Chernobyl thyroid cancers have demonstrated that the risk of thyroid carcinoma is strongly related to 131I dose absorbed by the thyroid.6-9 The reported excess relative risk per unit of dose (Gy) is between 2 and 5. In addition, age at exposure and iodine deficiency have been found to modify the 131I-related risk of thyroid cancer, with higher risk per unit of dose observed in persons exposed as younger children, particularly infants,10 and in individuals living in areas with low soil iodine content.7

Somatic genetic alterations that activate the mitogen-activated protein kinase signaling cascade are known to be “driver” mutations that play a crucial role in the development of PTC.11 These include point mutations in BRAF and RAS as well as chromosomal rearrangements such as RET/PTC (ret proto-oncogene/papillary thyroid carcinoma). Whereas mitogen-activated protein kinase activation via point mutations is far more common in sporadic thyroid cancer, in tumors associated with radiation exposure, this pathway is most frequently activated via RET/PTC and other chromosomal rearrangement. Studies of post-Chernobyl and postradiotherapy tumors have found RET/PTC rearrangements in up to 80% of PTCs.12-14 The link between chromosomal rearrangements and exposure to ionizing radiation has also been supported by studies that have demonstrated induction of RET/PTC in human thyroid cell lines and tissue xenografts in SCID (severe compromised immunodeficient) mice by x-ray or gamma-radiation.15, 16 Recent studies have led to better understanding of mechanisms by which radiation exposure induces chromosomal rearrangements. Studies of both RET/PTC and TRK (neurotrophic tyrosine kinase) rearrangements have shown that the gene loci involved in fusions lie in spatial proximity to one another within the human thyroid cell nucleus at the time of exposure,17-19 likely predisposing to recombination of adjacent chromosomal regions with radiation-induced DNA damage.

However, the association of chromosomal rearrangements or other mutational events with individual radiation doses in humans is not well established. Among PTCs that developed in individuals exposed predominantly to gamma-radiation from the atomic bombings in Hiroshima and Nagasaki, Japan, higher doses were associated with higher prevalence of RET/PTC rearrangements and lower prevalence of BRAF point mutations.20, 21 By contrast, no significant association between RET/PTC activation and individual 131I doses was found in one post-Chernobyl study of thyroid cancer occurring in the Bryansk oblast of the Russian Federation.22 The prevalence of another rearrangement type, PAX8/PPARγ (paired box 8/peroxisome proliferator-activated receptor gamma), known to occur in follicular thyroid carcinoma and only occasionally found in PTC,11 has not been studied in post-Chernobyl tumors.

Herein, we report the results of mutational analysis of a series of post-Chernobyl PTCs from Ukrainian individuals with measurement-based estimates of 131I dose to the thyroid gland reconstructed as part of the Ukrainian-American cohort study.23 Based on the aforementioned experimental and human data, we hypothesized that RET/PTC rearrangements represent a common genetic event in these cancers, and chromosomal rearrangements and point mutations have different association with 131I dose. The obtained results demonstrate the predominance of chromosomal rearrangements in these tumors, show for the first time the occurrence of PAX8/PPARγ rearrangements in post-Chernobyl tumors, and establish associations of specific genetic alterations with 131I doses and other patient characteristics.


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  2. Abstract

Patients and Tissue Samples

Cases included patients who were part of the Ukrainian-American cohort study and underwent surgery for suspected thyroid carcinoma.23 The cohort is composed of 13,243 Ukrainian residents, less than 18 years old at the time of the Chernobyl accident, with individual radioactivity measurements taken within 2 months after the accident. After 4 sequential screening examinations, 110 thyroid carcinomas, including 104 PTCs, were diagnosed between 1998 and 2008 at the Laboratory of Morphology of Endocrine System of the Institute of Endocrinology and Metabolism (IEM, Kyiv, Ukraine).24 The International Pathology Panel, within the Chernobyl Tissue Bank (CTB), reviewed all pathological diagnoses. A total of 75 of 104 cases of PTC had at least 1 frozen tissue specimen from which DNA or RNA were extracted at IEM or Imperial College (London, UK). Nucleic acids from 74 PTCs were received through the CTB. Four cases from a separate cohort exposed in utero were excluded, and 8 cases that lacked either DNA (n = 3) or RNA (n = 5) were also excluded.

Estimation of 131I Thyroid Doses

Dosimetric methods have been described in detail.25, 26 Briefly, individual 131I thyroid doses and their uncertainties were estimated from thyroid radioactivity measurements, data on dietary and lifestyle habits, and environmental transfer models using a Monte Carlo procedure with 1000 realizations per individual.26 For the analysis, we used the arithmetic mean of each individual's 1000 realizations as the best estimate of 131I dose corrected for thyroid masses typical of the Ukrainian population.6

Nucleic Acids

Tumor DNA and RNA were obtained through CTB. The samples were received with a CTB code that was later linked with a code for the individual in the Ukrainian-American study.23

Detection of Point Mutations

All tumors for which DNA was available were tested for point mutations in BRAF (Val600Glu, or V600E; and Lys601Glu, or K601E), NRAS (neuroblastoma RAS viral oncogene; codon 61), HRAS (Harvey rat sarcoma viral oncogene; codon 61), and KRAS (Kirsten rat sarcoma viral oncogene; codons 12/13) genes using fluorescence melting curve analysis as described.27 Briefly, the samples were amplified on the LightCycler (Roche Diagnostics, Indianapolis, Ind) using the LightCycler FASTStart DNA Master Mix (Roche) and specific probes.27 Post–polymerase chain reaction (PCR) melting curves were compared to those from control tumors known to have or those that lack specific point mutations. All mutations were confirmed by Sanger sequencing.

Detection of Chromosomal Rearrangements

All tumors for which RNA was available were screened for RET/PTC1, RET/PTC3, and PAX8/PPARγ using real-time reverse-transcription PCR. Tumor RNA was reverse-transcribed and amplified on the 7500 Real-Time PCR System (Applied Biosystems) using the TaqMan Universal PCR Master Mix (Applied Biosystems) and specific probes.27 For PAX8/PPARγ, a ΔCt of < 10 cycles, as compared with the amplification of GAPDH (glyceraldehyde-3-phosphate dehydrogenase), was used as a cutoff. For PAX8/PPARγ and RET/PTC3, the presence of the rearrangement was confirmed by conventional reverse-transcription PCR and agarose gel electrophoresis. All chromosomal rearrangements were confirmed by Sanger sequencing.

Statistical Analysis

Mutation prevalence data was analyzed using standard logistic regression modeling. The probability of a BRAF- or RAS-positive mutation for the relevant endpoint, given effect-modifying variables of age at surgery a, sex s, oblast O, and Chernobyl-associated radiation dose D (in Gy), is given by:

  • equation image

The model is fitted by binomial maximum likelihood28 using Epicure v 2.10 (1998). Age (at surgery) was centered by subtracting 25 years to aid convergence of fitted models. For chromosomal rearrangements, the linear and quadratic terms for dose (dose + dose2) were used, whereas for point mutations, a linear model in dose sufficed. Unless otherwise stated, all confidence intervals were profile-likelihood–based.28 All tests were 2-sided and based on the likelihood-ratio test.29 Adjustments were made for age at surgery, sex, oblast of residence at the time of screening, and dose because of indications of significant or borderline significant effects on mutation rates. Oblast at the time of screening compared with that at the time of exposure differed for only 3 patients. Tests of heterogeneity were performed as described by Pierce and Preston.30 Analysis of time from exposure to surgery used a standard linear regression model, using log-transformed time since exposure. All linear regression analyses were performed using Stata v 11.2 (2009). Mean age at exposure was compared by 2-sample t test.


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  2. Abstract

Case Characteristics

The case series includes 26 males (42%) and 36 females (58%) living in areas surrounding Chernobyl, ie, the Zhytomyr (n = 17; 27.4%), Kyiv (n = 12; 19.4%), or Chernihiv (n = 33; 53.2%) oblasts, who were between 5 months and 17 years old (mean, 8.0 years) at the time of the Chernobyl accident. The estimated 131I dose for patients in the study ranged from 0.008 Gy to 8.6 Gy, with a mean dose of 1.3 Gy. Surgical removal of PTCs occurred between October 1998 and December 2007, with time between exposure and surgery ranging from 12.5 to 21.6 years (mean, 16.5 years).

Mutation Analysis

RET/PTC rearrangement was the most common genetic alteration and was found in 22 (35%) cases, including 14 RET/PTC1 and 8 RET/PTC3 rearrangements (Table 1). Point mutations in the BRAF and RAS genes were found in 9 (15%) and 5 (8%) of the tumors, respectively. All 9 BRAF mutations were V600E. Four RAS mutations were detected in NRAS codon 61 and 1 in HRAS codon 61. No KRAS mutations were found in codons 12 and 13. In addition, we studied these tumors for PAX8/PPARγ rearrangement, a prototypic genetic alteration found in thyroid follicular carcinoma that occurs with lower prevalence in the follicular variant of PTC. Two tumors were positive for PAX8/PPARγ; both were of the follicular variant of PTC. In both cases, the fusions were between exon 9 of PAX8 and exon 1 of PPARγ, with several expected splice variants of the chimeric PAX8/PPARγ transcripts detected. One tumor had more than 1 mutation, harboring an NRAS point mutation in codon 61 and PAX8/PPARγ rearrangement. Twenty-five (40%) tumors revealed none of the studied mutations.

Table 1. Genetic Alterations and Exposure-Related Characteristics Among Cases of Thyroid Cancer Developed After the Chernobyl Accident in Ukraine
Genetic AlterationMutation Frequency131I Dose, Mean (Gy)Age at Exposure, Mean (y)Age at Surgery, Mean (y)Time Since Exposure, Mean (y)
  • a

    One case was found to be positive for both NRAS mutation and PAX8/PPARγ rearrangement.

RET/PTC114 (22%)1.046.423.616.9
RET/PTC38 (13%)1.546.320.113.7
BRAF9 (15%)0.2710.227.116.8
RASa5 (8%)0.2010.929.418.6
PAX8/PPARγa2 (3%)0.6212.225.813.5
No known mutation25 (40%)1.977.824.416.6
Total/overall62 (100%)

Univariate Analysis of Mutation Type and Exposure-Related Characteristics

Patients with tumors positive for BRAF or RAS point mutations had the lowest average dose of 131I (0.27 Gy and 0.20 Gy, respectively), significantly lower than that for all other patients (P < .001). Patients with tumors harboring RET/PTC1 or RET/PTC3 rearrangements received average doses of 1.04 Gy and 1.54 Gy, respectively. Patients with tumors negative for any of these mutations had the highest average dose (1.97 Gy). In addition, as compared with all other patients, patients with tumors harboring RET/PTC1 or RET/PTC3 were significantly younger at the time of exposure (6.4 and 6.3 years, respectively), whereas patients with BRAF or RAS mutations were significantly older at the time of exposure (10.2 and 10.9 years, respectively) (P = .01 for both). In our case series, age at exposure correlated with age at surgery (or attained age) (Pearson correlation coefficient, r2 = 0.85). Thus, patients with tumors having RET/PTC1 or RET/PTC3 rearrangements were also younger at the time of surgery (mean age 23.6 years and 20.1 years, respectively; P = .007) and patients with tumors positive for BRAF or RAS mutations were older (mean age 27.1 years and 29.4 years, respectively; P = .002) than other cases. The mean time between exposure and surgery for RET/PTC3-positive cases was 13.7 years, significantly shorter than that for all other cases combined (P < .001; Table 1).

Multivariate Analysis of Tumors Positive for BRAF or RAS Point Mutations

Factors independently associated with tumors harboring BRAF or RAS point mutations, as compared with all other tumors, are shown in Table 2. Adjusting for age at surgery, sex, and oblast of residence, there was a significant negative association between these point mutations and 131I dose (P = .001). The estimated regression coefficient for the dose term based on a log-linear model was −2.51 per Gy (95% confidence interval [CI] = −5.42, −0.78) (Fig. 1A). In addition, the point mutations were associated with older age at surgery (P = .014) and female sex (P = .002).

Table 2. Factors Associated With BRAF or RAS Point Mutations in Multivariate Analysis
CharacteristicBRAF or RAS MutationOdds Ratio95% Confidence Interval
% or mean (standard deviation)% or mean (standard deviation)
  • a

    Based on linear dose-response model.

Iodine-131 dose, Gy    
 0.008 to <0.0535.73.91.00Referent
 0.05 to <0.3528.619.60.19(0.01, 2.67)
 0.35 to 8.6035.776.50.09(0.01, 0.95)
P trend  0.001a 
Oblast of residence    
 Kyiv21.421.60.24(0.02, 2.33)
 Zhytomyr7.131.40.07(0.00, 0.82)
P heterogeneity  0.086 
Age at surgery, y28.1 (4.2)23.4 (4.9)1.26(1.05, 1.61)
P trend  0.014 
 Female85.752.921.59(2.69, 349.32)
P  0.002 
thumbnail image

Figure 1. Dose-response relationship is shown for point mutations and chromosomal rearrangements. (A) Dose-response relationship is shown for patients with tumors harboring BRAF or RAS point mutations. Odds ratio data for the relationship between chromosomal rearrangement and estimated 131I dose is fitted to a linear quadratic model with adjustments for sex, oblast, age of surgery, and dose. (B) Dose-response relationship is shown for patients with tumors harboring RET/PTC or PAX8/PPARγ chromosomal rearrangements. Odds ratio data for the relationship between chromosomal rearrangement and estimated 131I dose is fitted to a linear quadratic model with adjustments for sex, oblast, age of surgery, and dose.

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Multivariate Analysis of Tumors Positive for RET/PTC or PAX8/PPARγ Chromosomal Rearrangements

There was a significant negative dose-response relationship using continuous 131I dose (P = .040, not shown), which was improved at marginal levels of statistical significance (P = .053) by addition of a quadratic term in dose (dose2); overall, the linear-quadratic dose response was statistically significant (2 degrees of freedom [df] trend, P = .019; Table 3) with estimated regression coefficients of 0.96 per Gy (95% CI = −0.54, 2.61) for the linear term and of −0.31 per Gy2 for the quadratic term (95% CI = −0.70, 0.00). Although the parameters of the dose-response function are imprecise, as evidenced by wide confidence intervals, the data suggest a nonmonotone relationship for RET/PTC or PAX8/PPARγ-positive tumors and dose with increased risk at low-to-moderate doses and decreased risk at high doses (Fig. 1B). The latter may reflect the fact that cases with no known mutation were associated with the highest average dose overall (P = .012). Indeed, among the patients who received doses above 1.6 Gy, 10 of 16 (62.5%) had tumors that were negative for all mutations studied.

Table 3. Factors Associated With RET/PTC or PAX8/PPARγ Rearrangements in Multivariate Analysis
CharacteristicRET/PTC or PAX8/PPARγOdds Ratio95% Confidence Interval
% or mean (standard deviation)% or mean (standard deviation)
  • a

    Based on linear quadratic model (dose+dose2).

Iodine-131 dose, Gy    
 0.008 to <0.425.039.51.00Referent
 0.4 to <0.9125.023.71.38(0.28, 7.00)
 0.91 to <1.6325.010.52.47(0.34, 19.05)
 1.63 to 8.6025.026.30.77(0.13, 4.13)
P trend  0.019a 
Oblast of residence    
 Kyiv12.523.70.88(0.13, 5.13)
 Zhytomyr41.718.411.66(2.22, 82.52)
P heterogeneity  0.007 
Age at surgery, y22.6 (4.5)25.8 (5.3)0.79(0.66, 0.91)
P trend  0.001 
 Female45.863.20.27(0.06, 0.96)
P  0.043 

In addition to dose, the presence of chromosomal rearrangement was positively associated with residence in the Zhytomyr oblast (P = .007 for 2 df test of oblast differences) and negatively associated with female sex (P = .043) and attained age (P = .001; Table 3). The presence of RET/PTC3 rearrangement compared with all other tumors was negatively associated with time between exposure and surgery, even when adjusted for 131I dose and age at exposure (P = .001) or attained age (P = .012, not shown). No association with time from exposure to surgery was found for any other rearrangements or point mutations.

Comparison Between Tumors Positive for Point Mutations and Those Harboring Chromosomal Rearrangements

Direct comparison of tumors with point mutations (BRAF or RAS) and chromosomal rearrangements (RET/PTC or PAX8/PPARγ) using multivariate logistic regression demonstrated a significant difference in trends with 131I dose (P = .020; Table 4). In addition, age at surgery (P < .001), sex (P < .001), and oblast of residence (P = .003) were significantly and independently associated with presence of point mutations relative to chromosomal rearrangements. Compared with BRAF- or RAS-positive tumors, RET/PTC- or PAX8/PPARγ-positive tumors were likely to occur in individuals with higher 131I doses, younger age at surgery (and therefore younger at exposure), in males, and residents of Zhytomyr oblast (Table 4).

Table 4. Comparison of RET/PTC or PAX8/PPARγ Positive Tumors With BRAF or RAS Mutation Positive Tumors According to Selected Patient Characteristics in Thyroid Cancers That Developed After the Chernobyl Accident
CharacteristicRelative Risk of Rearrangements Versus Point Mutations (95% Confidence Interval)P Heterogeneitya
  • a

    Based on multivariate logistic regression models.

Iodine-131 dose, Gy8.00 (1.30, 150.96)0.020
Oblast of residenceKyiv vs Chernihiv 4.12 (0.22, 100.89)0.003
Zhytomyr vs Chernihiv 139.07 (7.13, 5843.00)
Age at surgery, y0.62 (0.47, 0.79)<0.001
SexFemale vs Male 0.01 (0.00, 0.16)<0.001


  1. Top of page
  2. Abstract

Our study of post-Chernobyl thyroid tumors confirms the high frequency of chromosomal rearrangements, particularly RET/PTC, and lower frequency of BRAF and RAS point mutations, compared with those typically observed in sporadic tumors,11 and reports for the first time the occurrence of PAX8/PPARγ rearrangements in post-Chernobyl PTCs. Moreover, we identified significant independent differences between chromosomal rearrangements and point mutations with respect to 131I thyroid doses, sex, oblast of residence, and age at exposure or surgery, suggesting that these tumors have distinct etiologies, ie, tumors with chromosomal rearrangements, but not with point mutations, are likely to be radiation-related.

Unique features of this study include individual 131I thyroid doses based on radioactivity measurements,6, 25 well-characterized tumors detected through standardized medical examinations, and comprehensive molecular profiling. Consequently, we were able to draw associations between specific mutation types and 131I dose and other contributing factors. The association between 131I thyroid dose and presence of chromosomal rearrangements followed a linear quadratic function, indicating a positive relationship in the low-to-moderate dose range and a negative relationship at high doses. It is likely that tumors with no known mutations accounted for the observed downturn at high doses, because their proportion relative to other tumors reached 62% at doses of 1.6 Gy or higher. Interestingly, a positive linear dose-response for RET/PTC rearrangements has been reported in thyroid tumors that developed among the atomic bomb survivors of Hiroshima and Nagasaki,20, 21 but very few cases in these studies received doses higher than 1.6 Gy.20 No significant association was found for RET/PTC activation with individual 131I doses in the study of PTCs from Bryansk oblast of the Russian Federation.22 However, these patients were exposed to lower doses (mean 0.363 Gy for childhood cancers and 0.039 Gy for adult cancers) than those in the current study (mean 1.3 Gy). The inconsistencies in dose-response findings across these studies are likely to result from different dose and age range, geographic origin, uncertainties in dose estimates, or limited sample size.

By contrast, patients with tumors harboring BRAF and RAS point mutations received the lowest average 131I doses, were oldest at the time of the Chernobyl accident or at surgery, and were predominantly female. The strong negative association for point mutation–positive tumors with dose found when comparing BRAF- and RAS-positive tumors against all others is consistent with that in atomic bomb survivors.20 The negative association with dose, together with the fact that the BRAF mutation is most commonly found in sporadic thyroid cancer,11, 31 incidence of which rapidly increases during the third decade of life and is 3 times as common in women,32, 33 suggest that BRAF- and RAS-positive tumors found in this cohort were likely to develop via pathogenic mechanisms more typical of sporadic thyroid cancer.

More than one-third of the tumors in our study had no identifiable mutations, and exhibited their own unique characteristics. The age at exposure or surgery for individuals with such tumors was higher than that of patients with RET/PTC rearrangements, but lower than that of patients with point mutations. These patients also received the highest 131I thyroid doses. Therefore, the development of these tumors is likely to be related to radiation exposure, but involved other, unknown mutations. A recent study of PTCs in atomic bomb survivors found ALK rearrangements, although they were detected at very low levels and using highly sensitive analyses, leaving the biological significance of this finding unclear.34 Other chromosomal rearrangements that occur very rarely in sporadic PTC but have been seen with higher frequency in PTCs following radiation exposure include BRAF/AKAP9 and TRK rearrangements.11 It is possible that these unmeasured, rare rearrangements may partially compose the set of tumors with as-yet unknown mutations in our study.

Another unexpected finding in the current study was a strong association between RET/PTC and residence in Zhytomyr oblast. Although study participants from Zhytomyr received higher 131I doses than those of the neighboring Ukrainian oblasts, the association with RET/PTC was independent of dose. The Zhytomyr oblast has no noticeable geographic or ethnic differences, but is associated with relative iodine deficiency. Indeed, residents of Zhytomyr have been shown to have lower levels of urinary iodine excretion than those of Kyiv or Chernihiv oblasts.35 Iodine deficiency has been shown to contribute to the risk of post-Chernobyl thyroid cancer in Belarus,3, 7 and may possibly be responsible for the increased frequency of RET/PTC in tumors from Zhytomyr oblast residents. It is conceivable that more avid trapping and intracellular metabolism of 131I by thyroid cells under conditions of high thyroid-stimulating hormone stimulation produce more extensive damage to the nuclear DNA, resulting in RET/PTC rearrangement. If confirmed, this would provide a biological basis for the higher risk of radiation-induced thyroid cancer in areas of relative iodine deficiency.

This study also identified PAX8/PPARγ rearrangements in post-Chernobyl tumors. This rearrangement is known to occur with high frequency in another type of thyroid cancer, follicular carcinoma, and with much lower frequency in the follicular variant of PTC.11 Both tumors positive for PAX8/PPARγ rearrangement in our study were the follicular variant of PTC. In one large study, tumors with PAX8/PPARγ rearrangements were more common in patients with a history of preceding nonthyroid cancer,36 which may implicate therapeutic radiation. However, to our knowledge, PAX8/PPARγ has not been previously reported in post-Chernobyl tumors or other radiation-associated cancers.

In summary, this study provides strong support for the association between chromosomal rearrangements and exposure to 131I from the Chernobyl accident. Furthermore, our findings point to a possible role of iodine deficiency in generation of RET/PTC rearrangements. Finally, because a significant proportion of tumors in our study had no detectable mutations and were associated with high radiation doses, we hypothesize that undiscovered mutational events important in radiation-induced thyroid carcinogenesis must exist.


  1. Top of page
  2. Abstract

Supported by National Institutes of Health (NIH) grant R01 CA88041 and in part by the Intramural Research Program of the NIH, National Cancer Institute.


The authors made no disclosure.


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  2. Abstract
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