Gastric juice MicroRNAs as potential biomarkers for the screening of gastric cancer

Authors

  • Long Cui MS,

    1. Department of Biochemistry and Molecular Biology, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, China
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  • Xinjun Zhang MD,

    Corresponding author
    1. Department of Gastroenterology, the Affiliated Hospital of Ningbo University School of Medicine, Ningbo, China
    • Xinjun Zhang, Department of Gastroenterology, the Affiliated Hospital of Ningbo University School of Medicine, Ningbo 315010, China

      Junming Guo, Department of Biochemistry and Molecular Biology, Ningbo University School of Medicine, Ningbo 315211, China

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    • Fax: (011) 86 574-87608638

  • Guoliang Ye MD,

    1. Department of Gastroenterology, the Affiliated Hospital of Ningbo University School of Medicine, Ningbo, China
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  • Tuo Zheng MD,

    1. Department of Gastroenterology, the Affiliated Hospital of Ningbo University School of Medicine, Ningbo, China
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  • Haojun Song BS,

    1. Department of Biochemistry and Molecular Biology, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, China
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  • Hongxia Deng MS,

    1. Department of Biochemistry and Molecular Biology, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, China
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  • Bingxiu Xiao MD,

    1. Department of Biochemistry and Molecular Biology, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, China
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  • Tian Xia BS,

    1. Department of Biochemistry and Molecular Biology, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, China
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  • Xiuchong Yu BS,

    1. Department of Biochemistry and Molecular Biology, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, China
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  • Yanping Le BS,

    1. Department of Biochemistry and Molecular Biology, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, China
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  • Junming Guo PhD

    Corresponding author
    1. Department of Biochemistry and Molecular Biology, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, China
    • Xinjun Zhang, Department of Gastroenterology, the Affiliated Hospital of Ningbo University School of Medicine, Ningbo 315010, China

      Junming Guo, Department of Biochemistry and Molecular Biology, Ningbo University School of Medicine, Ningbo 315211, China

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    • Fax: (011) 86-574-87608638


Abstract

BACKGROUND:

MicroRNAs (miRNAs) play a crucial role in carcinogenesis; however, it largely remains unclear whether miRNAs in gastric juice, which is specific for gastric tissues, can be used as biomarkers for gastric cancer. The objective of the current study was to investigate the feasibility of using gastric juice miRNAs as potential biomarkers to assist in screening for gastric cancer.

METHODS:

Gastric juice samples were collected from 141 patients who underwent upper gastrointestinal endoscopy examination between September 2010 and December 2011. Gastric cancer and adjacent normal biopsy specimens also were collected. The existence and stability of miRNAs in gastric juices were determined by real-time reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) and sequencing. miRNA levels in tissues and gastric juices were detected by RT-qPCR. A receiver operating characteristic (ROC) curve was constructed for differentiating gastric cancer from benign gastric diseases.

RESULTS:

Levels of miRNA-21 (miR-21) and miR-106a in gastric cancer tissues were significantly higher compared with the levels in adjacent tissues (P = .006 and P = .001, respectively). Patients who had gastric cancer had significantly different levels of gastric juice miR-21 and miR-106a compared with patients who had benign gastric diseases (both P < .001). There were significant correlations between miR-21/miR-106a levels and Borrmann types. miR-21 levels in intestinal type gastric cancer specimens were higher than that in diffuse (P = .003) or mixed (P < .001) gastric cancer types. The area under the ROC curve was up to 0.969 for miR-21 and 0.871 for miR-106a.

CONCLUSIONS:

The current results indicated that certain miRNAs in gastric juice are potential biomarkers that can assist in screening for gastric cancer. Cancer 2013. © 2013 American Cancer Society.

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