Gastric adenocarcinoma has a unique microRNA signature not present in esophageal adenocarcinoma

Authors

  • Zheng Chen MD, MS,

    1. Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee
    2. Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China
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  • Rama Saad BSc,

    1. Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee
    2. Department of Biology, The American University in Cairo, Cairo, Egypt
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  • Peilin Jia PhD,

    1. Department of Biomedical Informatics, Vanderbilt University Medical Center, Nashville, Tennessee
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  • DunFa Peng MD, PhD,

    1. Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee
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  • Shoumin Zhu PhD,

    1. Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee
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  • M. Kay Washington MD,

    1. Department Pathology, Vanderbilt University Medical Center, Nashville, Tennessee
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  • Zhongming Zhao PhD,

    1. Department of Biomedical Informatics, Vanderbilt University Medical Center, Nashville, Tennessee
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  • Zekuan Xu MD, PhD,

    Corresponding author
    1. Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China
    • Corresponding author: Wael El-Rifai, MD, Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, 1255 Light Hall, 2215 Garland Avenue, Nashville, TN 37232; Fax: (615) 322-7852; wael.el-rifai@vanderbilt.edu; or Zekuan Xu, MD, Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, China 210029; Fax: (011) 86-025-83724440; xuzekuan@njmu.edu.cn

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  • Wael El-Rifai MD, PhD

    Corresponding author
    1. Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee
    2. Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, Tennessee
    3. Department of Veterans Affairs, Tennessee Valley Healthcare System, Nashville, Tennessee
    • Corresponding author: Wael El-Rifai, MD, Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, 1255 Light Hall, 2215 Garland Avenue, Nashville, TN 37232; Fax: (615) 322-7852; wael.el-rifai@vanderbilt.edu; or Zekuan Xu, MD, Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, China 210029; Fax: (011) 86-025-83724440; xuzekuan@njmu.edu.cn

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  • The contents of this work are solely the responsibility of the authors and do not necessarily represent the official views of the National Cancer Institute, the Department of Veterans Affairs, or Vanderbilt University.

Abstract

BACKGROUND

MicroRNAs (miRNAs) play critical roles in tumor development and progression. The finding that a single miRNA can regulate hundreds of genes places miRNAs at critical hubs of signaling pathways. For the current study, the authors investigated the miRNA expression profile of gastric adenocarcinomas and compared it with esophageal adenocarcinomas to better identify a unique miRNA signature of gastric adenocarcinoma.

METHODS

miRNA expression profiles were obtained using 2 different proprietary microarray platforms on primary gastric adenocarcinoma tissue samples. The cross comparison of results identified 17 up-regulated miRNAs and 12 down-regulated miRNAs that overlapped in both platforms. Quantitative real-time polymerase chain reaction was performed for independent validation of a representative set of 8 miRNAs in gastric and esophageal adenocarcinomas compared with normal gastric mucosa or esophageal mucosa, respectively.

RESULTS

The deregulation of miR-146b-5p, miR-375, miR-148a, miR-31, and miR-451 was associated significantly with gastric adenocarcinomas. Conversely, deregulation of miR-21 (up-regulation) and miR-133b (down-regulation) was detectable in both gastric and esophageal adenocarcinomas. It was noteworthy that miR-200a was significantly down-regulated in gastric adenocarcinoma samples (P = .04) but was up-regulated in esophageal adenocarcinoma samples (P = .001). In addition, the expression level of miR-146b-5p displayed a strong correlation with the tumor stage of gastric cancer.

CONCLUSIONS

Gastric adenocarcinoma displayed a unique miRNA signature that distinguished it from esophageal adenocarcinoma. This specific signature may reflect differences in the etiology and/or molecular signaling in these 2 closely related cancers. The current findings suggest important miRNA candidates that can be investigated for their biological functions and for their possible diagnostic, prognostic, and therapeutic role in gastric adenocarcinoma. Cancer 2013;119:1985–1993. © 2013 American Cancer Society.

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