Detection of EGFR mutations and EML4-ALK rearrangements in lung adenocarcinomas using archived cytological slides (pages 370–376)
Natalia V. Mitiushkina, Aglaya G. Iyevleva, Artiom N. Poltoratskiy, Alexandr O. Ivantsov, Alexandr V. Togo, Igor S. Polyakov, Sergey V. Orlov, Dmitry E. Matsko, Viktor I. Novik and Evgeny N. Imyanitov
Article first published online: 13 FEB 2013 | DOI: 10.1002/cncy.21281
Epidermal growth factor receptor (EGFR) mutations and echinoderm microtubuleassociated protein-like 4-anaplastic lymphoma kinase (EML4-ALK) translocations were analyzed in archived cytological/histological sample pairs obtained from lung adenocarcinomas. Archived hematoxylin and eosin-stained smears and paraffin-embedded tissue blocks demonstrated similar performance both in terms of success rates for DNA/RNA isolation and for the ability to detect gene alterations.