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Cancer Cytopathology

Cover image for Vol. 121 Issue 7

July 2013

Volume 121, Issue 7

Pages 339–404

  1. Cytopathology Help Desk

    1. Top of page
    2. Cytopathology Help Desk
    3. Editorial
    4. Original Articles
    5. Correspondence
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  2. Editorial

    1. Top of page
    2. Cytopathology Help Desk
    3. Editorial
    4. Original Articles
    5. Correspondence
    1. You have free access to this content
      Standardizing preanalytical variables for molecular cytopathology (pages 341–343)

      Gilda da Cunha Santos

      Article first published online: 27 MAR 2013 | DOI: 10.1002/cncy.21290

      Standardized protocols for the fixation and handling of cytological specimens are required for reliable, consistent, and robust molecular test results for the assessment of biomarkers to direct patient selection for targeted therapies. This editorial discusses the strengths and limitations of the article by Dejmek et al with regard to this topic.

  3. Original Articles

    1. Top of page
    2. Cytopathology Help Desk
    3. Editorial
    4. Original Articles
    5. Correspondence
    1. You have free access to this content
      Preparation of DNA from cytological material : Effects of Fixation, Staining, and Mounting Medium on DNA Yield and Quality (pages 344–353)

      Annika Dejmek, Nooreldin Zendehrokh, Malgorzata Tomaszewska and Anders Edsjö

      Article first published online: 13 FEB 2013 | DOI: 10.1002/cncy.21276

      The effect of various clinically used techniques for the fixation, staining, and mounting of cytological samples on DNA yield and quality were tested using cultured cells treated as routine cytological samples. All the methods tested were found to result in analyzable DNA, but clear differences were observed, especially in liquid-based cytology samples.

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      Use of touch imprint cytology as a simple method to enrich tumor cells for molecular analysis (pages 354–360)

      Snjezana Dogan, Jillian C. Becker, Natasha Rekhtman, Laura H. Tang, Khedoudja Nafa, Marc Ladanyi and David S. Klimstra

      Article first published online: 10 APR 2013 | DOI: 10.1002/cncy.21292

      The neoplastic cell enrichment in touch imprints and matched frozen whole-tumor tissue samples were compared in 11 adenocarcinomas harboring KRAS mutations using light microscopy of hematoxylin and eosin-stained slides and the height ratio between the mutant and wild-type peaks on the sequencing electropherograms. The authors found that procurement of carcinomas by touch imprinting is superior to the procurement of frozen whole-tumor tissue when tumor DNA is needed for molecular analysis.

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      Comparative study of epidermal growth factor receptor mutation analysis on cytology smears and surgical pathology specimens from primary and metastatic lung carcinomas (pages 361–369)

      Renu Khode, Douglas A. Larsen, Brianne C. Culbreath, Shane Parrish, Kimberly L. Walker, Lubna Sayage-Rabie, Robert S. Beissner and Arundhati Rao

      Article first published online: 30 JAN 2013 | DOI: 10.1002/cncy.21273

      Epidermal growth factor receptor (EGFR) mutation status, which is used to qualify patients with non–small cell lung cancer for tyrosine kinase inhibitor therapy, depends on assay and platform selection as well as specimen type. Cytology smears can be used successfully as a specimen source for EGFR gene mutation analysis and can produce results comparable to those achieved with surgical specimens on both polymerase chain reaction and sequencing platforms.

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      Detection of EGFR mutations and EML4-ALK rearrangements in lung adenocarcinomas using archived cytological slides (pages 370–376)

      Natalia V. Mitiushkina, Aglaya G. Iyevleva, Artiom N. Poltoratskiy, Alexandr O. Ivantsov, Alexandr V. Togo, Igor S. Polyakov, Sergey V. Orlov, Dmitry E. Matsko, Viktor I. Novik and Evgeny N. Imyanitov

      Article first published online: 13 FEB 2013 | DOI: 10.1002/cncy.21281

      Epidermal growth factor receptor (EGFR) mutations and echinoderm microtubuleassociated protein-like 4-anaplastic lymphoma kinase (EML4-ALK) translocations were analyzed in archived cytological/histological sample pairs obtained from lung adenocarcinomas. Archived hematoxylin and eosin-stained smears and paraffin-embedded tissue blocks demonstrated similar performance both in terms of success rates for DNA/RNA isolation and for the ability to detect gene alterations.

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      EZH2 and CD79B mutational status over time in B-cell non-Hodgkin lymphomas detected by high-throughput sequencing using minimal samples (pages 377–386)

      Mauro Ajaj Saieg, William R. Geddie, Scott L. Boerner, Denis Bailey, Michael Crump and Gilda da Cunha Santos

      Article first published online: 29 JAN 2013 | DOI: 10.1002/cncy.21262

      Enhancer of zeste homolog 2 (EZH2) and B cell antigen receptor complex-associated protein beta chain (CD79B) mutations were detected over time by a MassARRAY spectrometry customized assay in B-cell non-Hodgkin lymphomas using samples on FTA cards as well as biopsies, archived smears, and cytospin preparations. Mutations varied in positive cases supporting that therapy should be based on results at the time of treatment and that testing minimal samples by high-throughput assays might facilitate studies in molecular events related to tumor progression and increase the material suitable for molecular analysis.

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      Evaluation of atypical urine cytology progression to malignancy (pages 387–391)

      Julianne Muus Ubago, Vikas Mehta, Eva M. Wojcik and Güliz A. Barkan

      Article first published online: 27 MAR 2013 | DOI: 10.1002/cncy.21278

      In a retrospective review of 1320 atypical urine samples from 851 patients collected from January 2000 to December 2011, upper tract urine specimens had the highest rate of progression to high-grade urothelial carcinoma at a rate of 55%. Thus, the authors postulate that the diagnosis of atypia in this specimen group has higher clinical significance, and these patients should be managed more aggressively.

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      uCyt+/ImmunoCyt and cytology in the detection of urothelial carcinoma: An update on 7422 analyses (pages 392–397)

      Evi Comploj, Christine Mian, Andrea Ambrosini-Spaltro, Christopher Dechet, Salvatore Palermo, Emanuela Trenti, Michele Lodde, Wolfgang Horninger and Armin Pycha

      Article first published online: 12 MAR 2013 | DOI: 10.1002/cncy.21287

      In the current study, the value of the uCyt1/ImmunoCyt test in the follow-up of patients with superficial urothelial carcinoma is confirmed. This could potentially reduce the number and cost of routine cystoscopic examinations performed in patients followed for bladder carcinoma.

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      Cytologic and cystoscopic predictors of recurrence and progression in patients with low-grade urothelial carcinoma (pages 398–402)

      Julie Jackson, Güliz A. Barkan, Umesh Kapur and Eva M. Wojcik

      Article first published online: 30 JAN 2013 | DOI: 10.1002/cncy.21272

      A combination of urine cytology and cystoscopic findings at the time of diagnosis of low-grade urothelial carcinoma is examined. Cystoscopic findings, including the presence of multiple lesions, as well as positive urine cytology at or shortly before the time of diagnosis of low-grade urothelial carcinoma, predicts disease recurrence or progression.

  4. Correspondence

    1. Top of page
    2. Cytopathology Help Desk
    3. Editorial
    4. Original Articles
    5. Correspondence
    1. You have free access to this content
    2. You have free access to this content

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