Expression of the transcription factors GATA3 and Pax2 during development of the mammalian inner ear
Article first published online: 28 DEC 2001
Copyright © 2002 Wiley-Liss, Inc.
Journal of Comparative Neurology
Volume 442, Issue 4, pages 378–391, 21 January 2002
How to Cite
Lawoko-Kerali, G., Rivolta, M. N. and Holley, M. (2002), Expression of the transcription factors GATA3 and Pax2 during development of the mammalian inner ear. J. Comp. Neurol., 442: 378–391. doi: 10.1002/cne.10088
- Issue published online: 28 DEC 2001
- Article first published online: 28 DEC 2001
- Manuscript Accepted: 23 OCT 2001
- Manuscript Revised: 25 JUN 2001
- Manuscript Received: 7 MAR 2001
- The Wellcome Trust. Grant Number: 056056
- cell differentiation;
The transcription factors GATA3 and Pax2 are expressed throughout development of the mouse inner ear. We have used antibodies to study their temporal and spatial expression patterns from embryonic days E8–E16.5. The two factors show reciprocal relationships in the regional patterning of the early otocyst and cellular patterning within the sensory epithelia. GATA3 is expressed in the whole otic placode at E8. In the otocyst at E9.5–10.5, the distribution is lateral and complementary to the medial expression pattern of Pax2. Only Pax2 is expressed in the endolymphatic duct, but both factors are expressed in the cochlea. At E11.5–13.5, GATA3 is expressed strongly in the cochlea, but in the dorsal, vestibular region it is downregulated. In all sensory epithelia, downregulation coincides with sensory innervation. Pax2 is expressed in all sensory and some nonsensory epithelia, but within sensory epithelia at E16.5 it is restricted to hair cells. GATA3 is expressed throughout key periods of cell proliferation, fate determination, and differentiation and is not specifically associated with any of these processes. Expression persists most strongly in the main components of the developing auditory system. These include the auditory sensory epithelium, the afferent and efferent nerves, and the mesenchymal and ectodermal cells in regions that form key parts of the middle and outer ear. GATA3 is thus expressed in functionally distinct groups of cells that integrate to form a complete sensory system. The results suggest that both factors may be involved in tissue compartmentalisation, morphogenesis, and cell signalling. J. Comp. Neurol. 442:378–391, 2002. © 2002 Wiley-Liss, Inc.