This study is dedicated to the memory of Carl-Magnus Johnsson, who tragically died from cerebral hemorrhage at the age of 29.
Ventricular proliferation zones in the brain of an adult teleost fish and their relation to neuromeres and migration (secondary matrix) zones†
Article first published online: 13 JUN 2001
Copyright © 2001 Wiley-Liss, Inc.
Journal of Comparative Neurology
Volume 436, Issue 1, pages 92–110, 17 July 2001
How to Cite
Ekström, P., Johnsson, C.-M. and Ohlin, L.-M. (2001), Ventricular proliferation zones in the brain of an adult teleost fish and their relation to neuromeres and migration (secondary matrix) zones. J. Comp. Neurol., 436: 92–110. doi: 10.1002/cne.1056
- Issue published online: 13 JUN 2001
- Article first published online: 13 JUN 2001
- Manuscript Accepted: 26 APR 2001
- Manuscript Revised: 8 FEB 2001
- Manuscript Received: 12 OCT 1998
- Swedish Natural Science Research Council. Grant Number: B-AA/BU 08554-312–315
- Royal Physiographic Society in Lund
- central nervous system;
- growth and embryonic development;
- cell division;
- neuronal plasticity
Zones containing actively dividing cells (proliferation zones: PZs), in the brain of adult three-spined sticklebacks, were identified by autoradiographic detection of 3H-thymidine and immunocytochemical detection of the thymidine analogue 5'-bromodeoxyuridine (BrdU), singly or in combination, and by immunocytochemical detection of proliferating cell nuclear antigen (PCNA) by monoclonal antibodies. The PZs are associated with boundaries between adult brain regions, as well as with defined morphofunctional subdivisions. PZs are located at the border between the telencephalon and diencephalon, and at the border between the mesencephalon and the rhombencephalon. In the midbrain, the PZ follows the dorsomedial, caudal, and ventrolateral aspects of each tectal hemisphere, extending over the caudal aspect of the torus semicircularis to the nucleus lateralis valvulae. In the hindbrain, the major PZ apparently represents the persisting embryonic secondary matrix layer of the developing cerebellum. In the forebrain, the PZs are associated with the ventricular zones of the olfactory bulbs and ventral telencephalic area (”subpallium”), dorsal telencephalic area (”pallium”), preoptic region, ventral thalamus, dorsal thalamus, epithalamus, pretectum, posterior tuberculum, and the hypothalamus. The diencephalic PZs are parcellated according to a neuromeric organisation (a synencephalic, a posterior, and an anterior parencephalic neuromere: p1, p2, and p3). The PZs of the secondary prosencephalon (telencephalon and hypothalamus) thus would belong to neuromeres p4–6, but do not form an immediately recognised serial pattern. The prosencephalic PZs correlate well with parts of embryonic migration areas as defined by Bergquist and Källén ( J. Comp. Neurol. 100:627–659), morphogenetic fields from which postmitotic neurones migrate to their final destination. J. Comp. Neurol. 436:92–110, 2001. © 2001 Wiley-Liss, Inc.