Fos-like immunohistochemical identification of neurons active during the startle response of the rainbow trout

Authors

  • Tom J. Bosch,

    1. Institute of Neurobiology, University of Amsterdam, 1098 SM Amsterdam, The Netherlands
    Current affiliation:
    1. Clinical Research, P.O. Box 90240, 5000 LT Tilburg, The Netherlands.
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  • Suharti Maslam,

    1. Institute of Neurobiology, University of Amsterdam, 1098 SM Amsterdam, The Netherlands
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  • Barry L. Roberts

    Corresponding author
    1. Trinity College Institute for Neuroscience and Department of Zoology, Trinity College, Dublin 2, Ireland
    • Department of Zoology, Trinity College, University of Dublin, Dublin 2, Ireland
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Abstract

Activity-dependent Fos-like expression was investigated immunohistochemically in rainbow trout (Oncorhynchus mykiss) that had performed vibratory-evoked startle responses. We found significantly higher numbers of Fos-like-immunoreactive neurons in the reticular formation, in the octavolateral area, and in several cranial nerve motor nuclei in the brain and in the motor column of the spinal cord of startled fish than in control fish. In one fish, in which stimulation did not evoke startle responses, substantial numbers of positive cells occurred in the brain, primarily in the magnocellular octavolateral nucleus. We observed Fos-like-immunoreactive neurons in cell groups that are known to participate in the startle response (e.g., the Mauthner cell) as well as in cell groups that have been proposed but until now not shown to be involved. J. Comp. Neurol. 439:306–314, 2001. © 2001 Wiley-Liss, Inc.

Ancillary