Differential distribution of olfactory receptor neurons in goldfish: Structural and molecular correlates
Version of Record online: 20 AUG 2004
Copyright © 2004 Wiley-Liss, Inc.
Journal of Comparative Neurology
Volume 477, Issue 4, pages 347–359, 27 September 2004
How to Cite
Hansen, A., Anderson, K. T. and Finger, T. E. (2004), Differential distribution of olfactory receptor neurons in goldfish: Structural and molecular correlates. J. Comp. Neurol., 477: 347–359. doi: 10.1002/cne.20202
- Issue online: 20 AUG 2004
- Version of Record online: 20 AUG 2004
- Manuscript Accepted: 3 APR 2004
- Manuscript Revised: 1 APR 2004
- Manuscript Received: 13 JAN 2004
- National Institutes of Health/National Institute on Deafness and Other Communication Disorders. Grant Numbers: R01 DC033 792, P30 DCO 4657
- vomeronasal organ
The olfactory system of many terrestrial vertebrates comprises a main olfactory organ and a vomeronasal organ each containing a morphologically distinct type of olfactory receptor neuron (ORN). The two cell types also differ in the expression of G-proteins and odorant receptor molecules. Fish do not have a vomeronasal organ, and their olfactory neurons—three different morphological types—are contained in one epithelium. The olfactory organ of goldfish appears as a rosette, with the sensory epithelium lying along the proximal portion of each lamella, where it attaches to the midline raphe. Using immunocytochemistry, in situ hybridization, and electron microscopy, we tested whether a correlation exists between receptor cell morphology, distribution of cell type within the sensory epithelium, and expression of odorant receptors and G-proteins. A strong correlation exists between ORN morphology, type of odorant receptor and G-protein expressed and the distribution of sensory cells within the olfactory epithelium. The Buck and Axel type of odorant receptor and Gαolf are expressed in tall ciliated ORNs distributed homogenously across the entire sensory epithelium. In contrast, microvillous ORNs expressing V2R-like receptors, and Gαo, Gαq, or Gαi-3, and crypt type ORNs expressing Gαo and Gαq, are preferentially located along the dorsal margin of the epithelium and near the midline raphe. V2R- and OR-type receptor molecules do not colocalize in one cell, and only crypt-type ORNs express more than one G-protein. J. Comp. Neurol. 477:347–359, 2004. © 2004 Wiley-Liss, Inc.