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ER81 and CaMKIV identify anatomically and phenotypically defined subsets of mouse olfactory bulb interneurons

Authors

  • Sachiko Saino-Saito,

    1. Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Yamagata, Japan 990-9585
    2. Weill Medical College, Cornell University at The Burke Medical Research Institute, White Plains, New York 10605
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  • John W. Cave,

    1. Weill Medical College, Cornell University at The Burke Medical Research Institute, White Plains, New York 10605
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  • Yosuke Akiba,

    1. Weill Medical College, Cornell University at The Burke Medical Research Institute, White Plains, New York 10605
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  • Hayato Sasaki,

    1. Weill Medical College, Cornell University at The Burke Medical Research Institute, White Plains, New York 10605
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  • Kaoru Goto,

    1. Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Yamagata, Japan 990-9585
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  • Kazuto Kobayashi,

    1. Department of Molecular Genetics, Institute of Biomedical Sciences, Fukushima Medical University, Fukushima, Japan 960-1295
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  • Roseann Berlin,

    1. Weill Medical College, Cornell University at The Burke Medical Research Institute, White Plains, New York 10605
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  • Harriet Baker

    Corresponding author
    1. Weill Medical College, Cornell University at The Burke Medical Research Institute, White Plains, New York 10605
    • Weill Medical College, Cornell University at The Burke Medical Research Institute, 785 Mamaroneck Avenue, White Plains, NY 10605
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Abstract

The mechanisms underlying dopamine (DA) phenotypic differentiation in the olfactory bulb (OB) have not yet been fully elucidated and are the subject of some controversy. OB DA interneurons destined for the glomerular layer were shown to originate in the subventricular zone (SVZ) and in the rostral migratory stream (RMS). The current study investigated whether calcium/calmodulin-dependent protein kinase IV (CaMKIV) either alone or together with the Ets transcription factor ER81 was necessary for phenotypic determination during migration of progenitors. In most brain areas, including the OB, CaMKIV and ER81 displayed a reciprocal distribution. In the SVZ, only ER81 could be demonstrated. In the RMS, a subpopulation of progenitors contained ER81, but few, if any, contained CaMKIV. In OB, CaMKIV expression, restricted to deep granule cells, showed limited overlap with ER81. ER81 expression was weak in deep granule cells. Strong labeling occurred in the mitral and glomerular layers, where ER81 colabeled dopaminergic periglomerular cells that expressed either tyrosine hydroxylase (TH) or green fluorescent protein, the latter reporter gene under control of 9-kb of 5′ TH promoter. Odor deprivation resulted in a significant 5.2-fold decline in TH immunoreactivity, but ER81 exhibited a relatively small 1.7-fold decline in immunoreactivity. TH expression as well as brain and bulb size were unchanged in CaMKIV knockout mice. These data suggest that ER81 may be required but is not sufficient for DA neuron differentiation and that CaMKIV is not directly involved in TH gene regulation. J. Comp. Neurol. 502:485–496, 2007. © 2007 Wiley-Liss, Inc.

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