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Article
Development of N-methyl-D-aspartate receptor subunits in avian auditory brainstem
Article first published online: 15 MAR 2007
DOI: 10.1002/cne.21303
Copyright © 2007 Wiley-Liss, Inc.
Additional Information
How to Cite
Tang, Y.-Z. and Carr, C. E. (2007), Development of N-methyl-D-aspartate receptor subunits in avian auditory brainstem. The Journal of Comparative Neurology, 502: 400–413. doi: 10.1002/cne.21303
Publication History
- Issue published online: 15 MAR 2007
- Article first published online: 15 MAR 2007
- Manuscript Accepted: 8 DEC 2006
- Manuscript Revised: 23 DEC 2005
- Manuscript Received: 5 AUG 2005
Funded by
- National Institutes of Health. Grant Numbers: DC00436, P30 DC0466 (to the University of Maryland Center for the Evolutionary Biology of Hearing)
- Abstract
- Article
- References
- Cited By
Keywords:
- cochlear nucleus;
- magnocellularis;
- laminaris;
- angularis;
- tonotopic gradient
Abstract
N-methyl-D-aspartate (NMDA) receptor subunit-specific probes were used to characterize developmental changes in the distribution of excitatory amino acid receptors in the chicken's auditory brainstem nuclei. Although NR1 subunit expression does not change greatly during the development of the cochlear nuclei in the chicken (Tang and Carr [2004] Hear. Res 191:79–89), there are significant developmental changes in NR2 subunit expression. We used in situ hybridization against NR1, NR2A, NR2B, NR2C, and NR2D to compare NR1 and NR2 expression during development. All five NMDA subunits were expressed in the auditory brainstem before embryonic day (E) 10, when electrical activity and synaptic responses appear in the nucleus magnocellularis (NM) and the nucleus laminaris (NL). At this time, the dominant form of the receptor appeared to contain NR1 and NR2B. NR2A appeared to replace NR2B by E14, a time that coincides with synaptic refinement and evoked auditory responses. NR2C did not change greatly during auditory development, whereas NR2D increased from E10 and remained at fairly high levels into adulthood. Thus changes in NMDA NR2 receptor subunits may contribute to the development of auditory brainstem responses in the chick. J. Comp. Neurol. 502:400–413, 2007. © 2007 Wiley-Liss, Inc.