Ultrastructural analysis of aminoglycoside-induced hair cell death in the zebrafish lateral line reveals an early mitochondrial response
Article first published online: 29 MAR 2007
Copyright © 2007 Wiley-Liss, Inc.
Journal of Comparative Neurology
Volume 502, Issue 4, pages 522–543, 1 June 2007
How to Cite
Owens, K. N., Cunningham, D. E., Macdonald, G., Rubel, E. W., Raible, D. W. and Pujol, R. (2007), Ultrastructural analysis of aminoglycoside-induced hair cell death in the zebrafish lateral line reveals an early mitochondrial response. J. Comp. Neurol., 502: 522–543. doi: 10.1002/cne.21345
- Issue published online: 29 MAR 2007
- Article first published online: 29 MAR 2007
- Manuscript Accepted: 25 JAN 2007
- Manuscript Revised: 6 NOV 2006
- Manuscript Received: 2 MAY 2006
- National Institute on Deafness and Other Communication Disorders (NIDCD). Grant Numbers: DC05987, DC06998, DC04661
- Virginia Merrill Bloedel Hearing Research Center
- transmission electron microscopy
Loss of the mechanosensory hair cells in the auditory and vestibular organs leads to hearing and balance deficits. To investigate initial, in vivo events in aminoglycoside-induced hair cell damage, we examined hair cells from the lateral line of the zebrafish, Danio rerio. The mechanosensory lateral line is located externally on the animal and therefore allows direct manipulation and observation of hair cells. Labeling with vital dyes revealed a rapid response of hair cells to the aminoglycoside neomycin. Similarly, ultrastructural analysis revealed structural alteration among hair cells within 15 minutes of neomycin exposure. Animals exposed to a low, 25-μM concentration of neomycin exhibited hair cells with swollen mitochondria, but little other damage. Animals treated with higher concentrations of neomycin (50–200 μM) had more severe and heterogeneous cellular changes, as well as fewer hair cells. Both necrotic-like and apoptotic-like cellular damage were observed. Quantitation of the types of alterations observed indicated that mitochondrial defects appear earlier and more predominantly than other structural alterations. In vivo monitoring demonstrated that mitochondrial potential decreased following neomycin treatment. These results indicate that perturbation of the mitochondrion is an early, central event in aminoglycoside-induced damage. J. Comp. Neurol. 502:522–543, 2007. © 2007 Wiley-Liss, Inc.