Phospholipase cβ4 expression reveals the continuity of cerebellar topography through development

Authors

  • Hassan Marzban,

    1. Department of Cell Biology and Anatomy, Genes and Development Research Group, The University of Calgary, Calgary, Alberta T2N 4N1, Canada
    2. Hotchkiss Brain Institute, Faculty of Medicine, The University of Calgary, Calgary, Alberta T2N 4N1, Canada
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  • Seunghyuk Chung,

    1. Department of Cell Biology and Anatomy, Genes and Development Research Group, The University of Calgary, Calgary, Alberta T2N 4N1, Canada
    2. Hotchkiss Brain Institute, Faculty of Medicine, The University of Calgary, Calgary, Alberta T2N 4N1, Canada
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  • Masahiko Watanabe,

    1. Department of Anatomy, Hokkaido University School of Medicine, Sapporo 060-8638, Japan
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  • Richard Hawkes

    Corresponding author
    1. Department of Cell Biology and Anatomy, Genes and Development Research Group, The University of Calgary, Calgary, Alberta T2N 4N1, Canada
    2. Hotchkiss Brain Institute, Faculty of Medicine, The University of Calgary, Calgary, Alberta T2N 4N1, Canada
    • Department of Cell Biology and Anatomy, Faculty of Medicine, University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta T2N 4N1, Canada
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Abstract

Mediolateral boundaries divide the mouse cerebellar cortex into four transverse zones, and within each zone the cortex is further subdivided into a symmetrical array of parasagittal stripes. Various expression markers reveal this complexity, and detailed maps have been constructed based on the differential expression of zebrin II/aldolase C in a Purkinje cell subset. Recently, phospholipase (PL) Cβ4 expression in adult mice was shown to be restricted to, and coextensive with, the zebrin II-immunonegative Purkinje cell subset. The Purkinje cell expression of PLCβ4 during embryogenesis and postnatal development begins just before birth in a subset of Purkinje cells that are clustered to form a reproducible array of parasagittal stripes. Double label and serial section immunocytochemistry revealed that the early PLCβ4-immunoreactive clusters in the neonate are complementary to those previously identified by neurogranin expression. The PLCβ4 expression pattern can be traced continuously from embryo to adult, revealing the continuity of the topographical map from perinatal to adult cerebella. The only exception, as has been seen for other antigenic markers, is that transient PLCβ4 expression (which subsequently disappears) is seen in some Purkinje cell stripes during the second postnatal week. Furthermore, the data confirm that some adult Purkinje cell stripes are composite in origin, being derived from two or more distinct embryonic clusters. Thus, the zone and stripe topography of the cerebellum is conserved from embryo to adult, confirming that the early- and late-antigenic markers share a common cerebellar topography. J. Comp. Neurol. 502:857–871, 2007. © 2007 Wiley-Liss, Inc.

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