Article

Early involvement of synapsin III in neural progenitor cell development in the adult hippocampus

  1. Hung-Teh Kao1,†,*,
  2. Ping Li1,
  3. Helen M. Chao1,
  4. Stephen Janoschka1,†,
  5. Kara Pham2,3,‡,
  6. Jian Feng4,
  7. Bruce S. Mcewen2,
  8. Paul Greengard5,
  9. Vincent A. Pieribone6,
  10. Barbara Porton1,†

Article first published online: 12 FEB 2008

DOI: 10.1002/cne.21643

Issue

The Journal of Comparative Neurology

The Journal of Comparative Neurology

Volume 507, Issue 6, pages 1860–1870, 20 April 2008

Additional Information

How to Cite

Kao, H.-T., Li, P., Chao, H. M., Janoschka, S., Pham, K., Feng, J., Mcewen, B. S., Greengard, P., Pieribone, V. A. and Porton, B. (2008), Early involvement of synapsin III in neural progenitor cell development in the adult hippocampus. J. Comp. Neurol., 507: 1860–1870. doi: 10.1002/cne.21643

Author Information

  1. 1

    Nathan Kline Institute for Psychiatric Research and the Department of Psychiatry, New York University School of Medicine, Orangeburg, New York 10962

  2. 2

    Laboratory of Neuroendocrinology, The Rockefeller University, New York, New York 10021

  3. 3

    Weissman Laboratory, Stanford University School of Medicine, Stanford, California 94305-5323

  4. 4

    Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, New York 14214

  5. 5

    Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, New York 10021

  6. 6

    The John B. Pierce Laboratory, Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06510

  1. †

    Department of Psychiatry, Division of Biology and Medicine, Brown University, 171 Meeting Street, Room 187, Providence, RI 02912

  2. ‡

    Department of Psychology, Barnard College, New York, NY 10027

*Department of Psychiatry, Division of Biology and Medicine, Brown University, 171 Meeting Street, Room 187, Providence, RI 02912

Publication History

  1. Issue published online: 12 FEB 2008
  2. Article first published online: 12 FEB 2008
  3. Manuscript Accepted: 19 DEC 2007
  4. Manuscript Revised: 1 OCT 2007
  5. Manuscript Received: 28 JUN 2007

Funded by

  • National Institutes of Health. Grant Numbers: MH070898, NS047209

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Keywords:

  • neurogenesis;
  • proliferation;
  • differentiation;
  • PSA-NCAM;
  • Ki67;
  • PCNA;
  • immunocytochemistry;
  • dentate gyrus

Abstract

Synapsin III is a synaptic vesicle-associated protein that is expressed in cells of the subgranular layer of the hippocampal dentate gyrus, a brain region known to sustain substantial levels of neurogenesis into adulthood. Here we tested the hypothesis that synapsin III plays a role in adult neurogenesis with synapsin III knockout and wild-type mice. Immunocytochemistry of the adult hippocampal dentate gyrus revealed that synapsin III colocalizes with markers of neural progenitor cell development (nestin, PSA-NCAM, NeuN, and Tuj1) but did not colocalize with markers of mitosis (Ki67 and PCNA). Because neurogenesis consists of a number of stages, the proliferation, survival, and differentiation of neural progenitor cells were systematically quantitated in the hippocampal dentate gyrus of adult synapsin III knockout and wild-type mice. We found a 30% decrease in proliferation and a 55% increase in survival of neural progenitor cells in synapsin III knockout mice. We also observed a 6% increase in the number of neural progenitor cells that differentiated into neurons. No difference in the volume of the dentate gyrus was observed between synapsin III knockout and wild-type mice. Collectively, our results demonstrate a novel role for synapsin III in regulating the proliferation of neural progenitor cells in the adult hippocampal dentate gyrus. These findings suggest a distinct function for this synaptic vesicle protein, in addition to its role in neurotransmission. J. Comp. Neurol. 507:1860–1870, 2008. © 2008 Wiley-Liss, Inc.

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