Regressive events in rat corticospinal axons during development in in vitro slice cocultures: Retraction, amputation, and degeneration
Article first published online: 6 JAN 2009
Copyright © 2009 Wiley-Liss, Inc.
Journal of Comparative Neurology
Volume 513, Issue 2, pages 164–172, 10 March 2009
How to Cite
Yoshioka, N., Murabe, N. and Sakurai, M. (2009), Regressive events in rat corticospinal axons during development in in vitro slice cocultures: Retraction, amputation, and degeneration. J. Comp. Neurol., 513: 164–172. doi: 10.1002/cne.21950
- Issue published online: 6 JAN 2009
- Article first published online: 6 JAN 2009
- Manuscript Accepted: 19 NOV 2008
- Manuscript Revised: 14 OCT 2008
- Manuscript Received: 20 JUN 2008
- Ministry of Education, Culture, Sports, Science and Technology of Japan. Grant Numbers: 18021034, 20300138
Additional Supporting Information may be found in the online version of this article.
|cne_21950_sm_SupFig1.doc||22K||Fig. S1. Branch regression length are not different between ages in culture studied. Average length of branch regression is shown (average ± s.e.m.). Regression events with the length of more than 0 μm and less than 300 μm are included. There were 4 regression events with the length of more than 370 μm. Those exceptionally large regressions were excluded.|
|cne_21950_sm_SupFig2.doc||33K||Fig. S2. Branch regression very often stops at the branch point. In Fig. 2C, data points accumulated on y=x line. This means that a number of branches completely regressed up to the branch point within a single day without further regression that went beyond the branch point. To show this more explicitly, we generated histograms for the lengths of the branches that showed regression (B, C). (A) Solid lines in the diagrams indicate the part of the axon existing at the indicated time points, while the dotted line is the part that disappeared within 1 day (the part that regressed within 1 day). Br1 (branch 1) is the axonal branch analyzed, and Br2 (branch 2) is the sister branch of Br1. L: Starting Br1 length, M: Length of Br1 on the next day, N: Length of the regression of Br1 in a day. (B) Histogram of the length of Br1 on the next day (=M) when branch length at the start was 0 to 20 μm. Those short branches most frequently showed regression of -5 to 5 μm. This reflects the fact that complete regression up to the branch point occurred very frequently. (C) Histogram of the length of Br1 on the next day (=M) when the branch length at the start was 20 to 80 μm. The results are basically the same as B, although regression length of the branches varied widely (D).|
|cne_21950_sm_SupFig3.doc||26K||Fig. S3. Summary of the average percent change in CV before and after the onset of degeneration (mean±.s.e.m., N=6).|
|cne_21950_sm_SupMovie1.mov||872K||Supplemental Movie 1. Developmental rapid axonal degeneration (DRAD) of cortical axons in the spinal cord was monitored at 10 DIV. The arrowheads indicate a degenerating axon. The arrow indicates one of axon branches which is growing.|
|cne_21950_sm_SupMovie2.mov||228K||Supplemental Movie 2. Axonal retraction followed by a branch selective DRAD (shown by the arrow) was monitored at 6 DIV. DRAD was selective to one of the branches in an axon still showing growth of the branches.|
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