The last two authors contributed equally to this work.
Equal numbers of neuronal and nonneuronal cells make the human brain an isometrically scaled-up primate brain
Version of Record online: 18 FEB 2009
Copyright © 2009 Wiley-Liss, Inc.
Journal of Comparative Neurology
Volume 513, Issue 5, pages 532–541, 10 April 2009
How to Cite
Azevedo, F. A.C., Carvalho, L. R.B., Grinberg, L. T., Farfel, J. M., Ferretti, R. E.L., Leite, R. E.P., Filho, W. J., Lent, R. and Herculano-Houzel, S. (2009), Equal numbers of neuronal and nonneuronal cells make the human brain an isometrically scaled-up primate brain. J. Comp. Neurol., 513: 532–541. doi: 10.1002/cne.21974
- Issue online: 18 FEB 2009
- Version of Record online: 18 FEB 2009
- Manuscript Accepted: 16 DEC 2008
- Manuscript Revised: 15 SEP 2008
- Manuscript Received: 6 JUN 2008
- IIEP-Albert Einstein
- Alexander von Humboldt Foundation
- brain size;
- neuron numbers;
- glia/neuron ratio;
- comparative neuroanatomy
The human brain is often considered to be the most cognitively capable among mammalian brains and to be much larger than expected for a mammal of our body size. Although the number of neurons is generally assumed to be a determinant of computational power, and despite the widespread quotes that the human brain contains 100 billion neurons and ten times more glial cells, the absolute number of neurons and glial cells in the human brain remains unknown. Here we determine these numbers by using the isotropic fractionator and compare them with the expected values for a human-sized primate. We find that the adult male human brain contains on average 86.1 ± 8.1 billion NeuN-positive cells (“neurons”) and 84.6 ± 9.8 billion NeuN-negative (“nonneuronal”) cells. With only 19% of all neurons located in the cerebral cortex, greater cortical size (representing 82% of total brain mass) in humans compared with other primates does not reflect an increased relative number of cortical neurons. The ratios between glial cells and neurons in the human brain structures are similar to those found in other primates, and their numbers of cells match those expected for a primate of human proportions. These findings challenge the common view that humans stand out from other primates in their brain composition and indicate that, with regard to numbers of neuronal and nonneuronal cells, the human brain is an isometrically scaled-up primate brain. J. Comp. Neurol. 513:532–541, 2009. © 2009 Wiley-Liss, Inc.