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CNE_22192_sm_SupFig1.tif4880KFigure S1. Parasagittal organization of the pigeon flocculus. A shows a photograph of the posterior pigeon cerebellum, indicating the vestibulocerebellum (VbC; folia IXcd and X), as well as their lateral extension forming the auricle (Au). B shows the Purkinje cell response properties in folium IXcd. The flocculus, the lateral portion, responds best to rotational optic flow about either the vertical axis (rVA; green) or an horizontal axis oriented 45° to midline (rH45; blue; Graf et al., 1988; Wylie and Frost, 1993). Zones in the medial part of the ventral uvula and nodulus (teal, orange and yellow), responds best to translational optic flow stimuli (see Wylie and Frost, 1999). C shows a photomicrograph of zebrin expression in the pigeon cerebellum (shown as magenta), illustrating subsets of Purkinje cells that are immunopositive for zebrin (ZII+) separated by subsets of Purkinje cells that are immuonegative (ZII-). D and E illustrate the pattern of zebrin II expression in the pigeon posterior cerebellum (shown as magenta; adapted from Pakan et al., 2007), shown with a coronal section through the posterior cerebellum (D) and a schematic of the pattern of zebrin II positive stripes (E). The zebrin stripes are numbered from 1-7 through folium IXcd. Shown in F, the rVA and rH45 cells are organized into four zones in the flocculus: two rVA zones (0 and 2, green) interdigitated with two rH45 zones (1 and 3, blue). The rVA and rH45 zones receive climbing fiber input from the caudal and rostral medial column of the inferior olive (mcIO), respectively. The caudal mcIO projects to the P4+/- (zone 0) and the P6+/- zebrin stripes (zone 2) and the rostral mcIO projects to the P5+/- (zone 1) and the P7+/- zebrin stripes (zone 3). dl, vl, dorsal and ventral lamellae of the inferior olive; gl, granular layer; ml, molecular layer; pcl, Purkinje cell layer. A red-green copy of this figure is available as text Figure 1. Scale bars A,D,E (applies also to B)= 1mm, C =100μm.
CNE_22192_sm_SupFig2.tif4824KFigure S2. Pattern of terminal labeling in the inferior olive from injections in the nucleus lentiformis mesencephali (LM) and the nucleus of the basal optic root (nBOR). A shows a graph of the rostro-caudal extent of terminal labeling observed in the medial column of the inferior olive (mcIO) resulting from injections of anterograde tracers into the nucleus lentiformis mesencephali (LM; green bars) and the nucleus of the basal optic root (nBOR; magenta bars). The bars represent the presence of terminal labeling in the mcIO in the corresponding rostro-caudal region of the inferior olive, normalized and averaged over all cases. Thin magenta bars represent a comparatively small amount of labeling more caudal in the mcIO from nBOR injections. B is a photomicrograph of terminal labeling in the ipsilateral caudal mcIO from a green injection in LM and a red injection (shown as magenta in figure) in nBOR. Note the amount of terminal labeling from nBOR is small and is located laterally in the mcIO. C is a photomicrograph of labeling from a green injection in LM illustrating terminal labeling in the medial and lateral regions of the mcIO. There was no terminal labeling observed in the contralateral mcIO from injections in LM. D and E are photomicrographs of terminal labeling in the rostral mcIO from a red injection (shown as magenta in figure) in nBOR; note the heavy terminal labeling in the medial portions of the mcIO (D and E, left) and the presence of a small amount of terminal labeling in the contralateral mcIO in E (right), located slightly lateral compared to the ipsilateral labeling. E also shows fibers crossing the midline ventral to the raphe nucleus (R). Dotted lines represent midline; broken lines represent the position of the twelfth cranial nerve, which is used as a landmark for the lateral edge of the mcIO. A red-green copy of this figure is available as text Figure 3. Scale bars in B-E = 100μm
CNE_22192_sm_SupFig3.tif4666KFigure S3. Typical injection sites in nucleus lentiformis mesencephali (LM) and the nucleus of the basal optic root (nBOR) and the resulting pattern of mossy fiber (MF) labeling in folium IXcd. A and B show photomicrographs of typical injection sites in nBOR (A; magenta) and LM (B;green). C-E show photomicrographs of typical resulting MF terminals (rosettes) in folium IXcd from injections in nBOR (red/magenta in figure) and LM (green). In C and D, note the proximity of the MF terminals in the superficial granular cell layer (gl), directly adjacent to the Purkinje cell layer (pcl) in both the dorsal lamella (C) and the ventral lamella (D). In E, it can be seen that the MF terminal pattern is organized in parasagittally oriented clusters in the granular layer. For abbreviations see list. A red-green copy of this figure is available as text Figure 4. Scale bars: A,B = 500μm; C-E = 100μm.
CNE_22192_sm_SupFig4.tif4534KFigure S4. Pattern of mossy fiber (MF) labeling in folium IXcd from injections in nucleus lentiformis mesencephali (LM) and the nucleus of the basal optic root (nBOR), and zebrin expression in folium IXcd. A-C show photomicrographs of the typical pattern of resulting MF terminals (rosettes) in folium IXcd from injections in nBOR (red/magenta in figure) and LM (green). A shows an example of parasagittal clusters of labeled MF terminals in the dorsal lamella and B shows an example of parasagittal clusters of labeled MF terminals in the ventral flocculus from injections in nBOR (red/magenta in figure) and LM (green). C shows a single parasagittal zone in the dorsal portions of the auricle (Au) from an injection in LM. D and E are examples of zebrin expression in folium IXcd shown in magenta. D shows the “?” zebrin immunopositive zone, dividing the P1- zone into medial and lateral regions, and E shows the natural paucity of Purkinje cells (arrowhead) in the mid region of the P2+ zebrin immunopositive zone, dividing the P2+ zone into medial and lateral regions. Dotted lines represent the boundaries of the granular cell layer. gl, granular layer; ml, molecular layer; Pcl, Purkinje cell layer; wm, cerebellar white matter. A red-green copy of this figure is available as text Figure 5. All scale bars are 100μm.
CNE_22192_sm_SupFig5.tif4981KFigure S5. Reconstruction of MF terminals resulting from injections in the nucleus lentiformis mesencephali (LM) from Case#5. B shows the injection site in a photomicrograph of LM. See Figure 6 caption for details of A,C and D. A red-green copy of this figure is available as text Figure 7. Scale bar in A= 1mm, B = 200μm.
CNE_22192_sm_SupFig6.tif4983KFigure S6. Reconstruction of MF terminals resulting from injections in the nucleus lentiformis mesencephali (LM) and the nucleus of the basal optic root (nBOR) from Case#5. A and B show photomicrographs of the typical pattern of resulting MF terminals (rosettes) in folium IXcd from injections in nBOR (green) and LM (red/magenta in figure) and the zebrin expression. In A, note the correspondence between the zebrin positive stripes (P2+ and P4+; magenta in Purkinje cell layer and molecular layer) and the MF terminals of LM and nBOR in the granular layer. B shows the region of folium IXcd directly surrounding the midline (dotted line) and the P1+ zebrin stripe (green in Purkinje cell layer and molecular layer); note the absence of MF terminal labeling in the ipsilateral dorsal lamella. gl, granular layer; ml, molecular layer; Pcl, Purkinje cell layer; wm, cerebellar white matter. See Figure 6 caption for details on C and D. A red-green copy of this figure is available as text Figure 8. Scale bars in A and B = 200μm.

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