Additional Supporting Information may be found in the online version of this article.

CNE_22242_sm_Supplfig1.tif366KSupplementary Figure 1: Measurements of the total number of nuclei in different regions of injured spinal cord cross-sections sampled as described in the methods. The total number of cells positive for Draq5 (a nuclear marker) were counted in the spared white matter (SWM; A), white matter lesion borders (WMLB; B), spared gray matter (SGM; C), and gray matter lesion borders (GMLB; D). The counts were conducted in sections 2.25mm rostral (R) and caudal (C) to the lesion epicenter as most changes in PPAR-δ cell numbers occurred in these distal segments. The counts indicate that though there is a general trend toward increased cell numbers after injury versus naïve and in lesion borders compared to outlying spared tissue, changes in overall cell numbers do not directly correspond to changes in PPAR-δ cell numbers. For instance, total cell numbers increase in GMLB between 14d and 28d post-injury yet the number of PPAR-δ+ cells decreases during this same time. N=Naïve; d=day post injury. *p<0.05, **p<0.01, ***p<0.001
CNE_22242_sm_Supplfig2.tif19184KSupplementary Figure 2: PPAR-δ+ oligodendrocytes (OLs) increase after SCI. A: PPAR-δ+ CC1 cells decreased slightly at 1dpi in white matter (WM). At 7d and 14dpi, PPAR-δ+ OLs had significantly increased in WM lesion border (WMLB) compared to 1dpi WMLB. Further, PPAR-δ+ CC1 cells along the WMLB at 14dpi were significantly greater than naïve WM and spared SWM within the same sections. B: PPAR-δ+ OLs were significantly reduced at 1dpi in gray matter lesion borders (GMLB) followed by a subsequent rise at 7d and 14dpi. C: PPAR-δ+ OL distribution in WM at 14dpi revealed the highest numbers were present in the WMLB at the epicenter and caudal sections. D: The number of PPAR-δ+ OLs had doubled by 7dpi compared to naïve. At 14dpi, the total number of OLs and the number of OLs with PPAR-δ+ nuclei were both significantly increased.
CNE_22242_sm_Supplfig3.tif9566KSupplementary Figure 3: PPAR-δ is present in neurons after SCI. A-C: Confocal images of double-labeling for PPAR-δ (red) and NeuN (green), a neuronal marker, revealed co-localization in SCI tissue in almost all neurons in spared gray matter. Image is from 14dpi tissue, 2.25 mm caudal to epicenter. Scale bar = 20 μm.

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