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CNE_22245_sm_SuppFig1.tif2247KSupplemental Fig. 1 Preabsorbtion of anti-SOM with SOM-14 and CST-14 blocks immunohistochemical staining Twenty micron sections of paraformaldehyde-fixed E14 ciliary ganglia were stained with A) 1:100 dilution of rat mAb YC7 anti-SOM; B) 1:100 dilution of anti-SOM plus 2 mM synthetic SOM-14; C) 1:100 dilution of anti-SOM plus 2 mM synthetic CST-14; or D) blocking solution alone. Binding of the primary antibody was visualized with donkey anti-rat-Cy3 as described in Materials and Methods. Addition of either peptide completely blocked staining due to the primary antibody. Photographs were acquired on a Nikon C1 confocal at identical laser intensities and magnifications for all samples and the images transferred to Adobe Photoshop. The montage was created by adjusting images to the desired size and resolution, then the image was flattened, changed to grayscale, and brightness and intensity of all panels were adjusted concomitantly so that staining in Panel A could be clearly seen. Calibration bar = 50 μ
CNE_22245_sm_SuppFig2.tif2170KSupplemental Fig. 2 Specificity of the PSS1 and PSS2 probes. PSS1 antisense probe (A) and PSS1 sense probe (C) were synthesized by incorporating digoxigenin-labeled UTP and hybridized on transverse sections of the trunk region of an E8 embryo. Arrows point to the sympathetic ganglia. PSS2 antisense probe (B) and PSS2 sense probe (D) were hybridized on coronal sections through the head of an E12 chicken embryo. Staining with PSS1 and PSS2 sense probes was undetectable over background. Calibration bars in each panel = 100 μ

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