The cortistatin gene PSS2 rather than the somatostatin gene PSS1 is strongly expressed in developing avian autonomic neurons
Version of Record online: 13 OCT 2009
Copyright © 2009 Wiley-Liss, Inc.
Journal of Comparative Neurology
Volume 518, Issue 6, pages 839–850, 15 March 2010
How to Cite
Nishi, R., Stubbusch, J., Hulce, J. J., Hruska, M., Pappas, A., Bravo, M.-C., Huber, L. P., Bakondi, B., Soltys, J. and Rohrer, H. (2010), The cortistatin gene PSS2 rather than the somatostatin gene PSS1 is strongly expressed in developing avian autonomic neurons. J. Comp. Neurol., 518: 839–850. doi: 10.1002/cne.22245
- Issue online: 13 JAN 2010
- Version of Record online: 13 OCT 2009
- Accepted manuscript online: 13 OCT 2009 12:00AM EST
- Manuscript Accepted: 2 OCT 2009
- Manuscript Revised: 29 JUL 2009
- Manuscript Received: 13 FEB 2009
- NIH. Grant Numbers: NS25767, DA17784
- Deutsche Forschungs Gemeinschaft. Grant Number: RO2551/1-1
- Schram-Foundation. Grant Number: T287/16252
Additional Supporting Information may be found in the online version of this article.
|CNE_22245_sm_SuppFig1.tif||2247K||Supplemental Fig. 1 Preabsorbtion of anti-SOM with SOM-14 and CST-14 blocks immunohistochemical staining Twenty micron sections of paraformaldehyde-fixed E14 ciliary ganglia were stained with A) 1:100 dilution of rat mAb YC7 anti-SOM; B) 1:100 dilution of anti-SOM plus 2 mM synthetic SOM-14; C) 1:100 dilution of anti-SOM plus 2 mM synthetic CST-14; or D) blocking solution alone. Binding of the primary antibody was visualized with donkey anti-rat-Cy3 as described in Materials and Methods. Addition of either peptide completely blocked staining due to the primary antibody. Photographs were acquired on a Nikon C1 confocal at identical laser intensities and magnifications for all samples and the images transferred to Adobe Photoshop. The montage was created by adjusting images to the desired size and resolution, then the image was flattened, changed to grayscale, and brightness and intensity of all panels were adjusted concomitantly so that staining in Panel A could be clearly seen. Calibration bar = 50 μ|
|CNE_22245_sm_SuppFig2.tif||2170K||Supplemental Fig. 2 Specificity of the PSS1 and PSS2 probes. PSS1 antisense probe (A) and PSS1 sense probe (C) were synthesized by incorporating digoxigenin-labeled UTP and hybridized on transverse sections of the trunk region of an E8 embryo. Arrows point to the sympathetic ganglia. PSS2 antisense probe (B) and PSS2 sense probe (D) were hybridized on coronal sections through the head of an E12 chicken embryo. Staining with PSS1 and PSS2 sense probes was undetectable over background. Calibration bars in each panel = 100 μ|
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