Compartmentalization of the precheliceral neuroectoderm in the spider Cupiennius salei: Development of the arcuate body, optic ganglia, and mushroom body
Version of Record online: 16 FEB 2010
Copyright © 2010 Wiley-Liss, Inc.
Journal of Comparative Neurology
Volume 518, Issue 13, pages 2612–2632, 1 July 2010
How to Cite
Doeffinger, C., Hartenstein, V. and Stollewerk, A. (2010), Compartmentalization of the precheliceral neuroectoderm in the spider Cupiennius salei: Development of the arcuate body, optic ganglia, and mushroom body. J. Comp. Neurol., 518: 2612–2632. doi: 10.1002/cne.22355
- Issue online: 28 APR 2010
- Version of Record online: 16 FEB 2010
- Accepted manuscript online: 16 FEB 2010 12:00AM EST
- Manuscript Accepted: 28 JAN 2010
- Manuscript Revised: 15 JAN 2010
- Manuscript Received: 12 NOV 2009
- German Research Foundation
Additional Supporting Information may be found in the online version of this article.
|CNE_22355_sm_suppfig1.tif||32199K||Supplementary Fig. 1(A-B): Structure of the early larval brain of Cupiennius salei. Light micrographs of parasagittal sections through the brain of a larva (1st larval stage) stained with Toluidine. (A) Medial sections, (B) lateral section. See text and figure legend 2 for further details. ABco, arcuate body cortex; chn, cheliceral neuromere; l1n, neuromere associated with the segment of walking leg 1; MBco, mushroom body cortex; MBnp, mushroom body neuropile; MBco GBp, mushroom body cortex, globuli precursors; ms, muscles; oe, oesophagus; OCco, optic centre cortex; OCmnp, median optic centre neuropile; OClnp, lateral optic centre neuropile; pedn, pedipalpal neuromere; PME, posterior median eye. PRCco, protocerebral cortex; PRCnp, protocerebral neuropile; PRCpg, protocerebral precursor groups; VNCnp, ventral nerve cord neuropile. Scale bar: (A) 150 μm in panel A,B.|
|CNE_22355_sm_suppfig2-3.tif||19628K||Supplementary Fig. 2: Neural precursor groups arise in the surface of the PLE/ALE groove (see also supplementary movie 1). Confocal micrograph of a maximum projection of optical horizontal sections from 28 to 62 μm; anterior is towards the top, medial towards the left. The arrows point to neural precursor groups in the groove. Supplementary Figure 3 A-B: dachshund and Prospero are co-expressed in the developing mushroom bodies. Merged confocal and light micrographs of embryos stained with a DIG-labelled dac RNA probe (dark green) and the Cupiennius salei anti-Prospero antibody (magenta). (A) The arrow points to Prospero positive cells in the ring-shaped dac domain that corresponds to the mushroom body vesicle in the anterior area of the developing protocerebrum. At 180 hours only few cells co-express Prospero and Csdac in the ring-shaped structure of the developing mushroom body. (B) The arrow points to the additional dac domain posterior to the ring-shaped domain. Many cells in this posterior domain express Prospero. Additonal cells co-express Prospero and Csdac in the ring-shaped structure (arrowheads). MB, developing mushroom body.|
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