SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
CNE_22422_sm_suppinfofigure.tif6206KDark-field photomicrographs of in situ hybridization sections demonstrate the absence of labeling by using control sense in situ hybridization probes for TGF-βs. A: The presence of TGF-β1 mRNA is shown in the paraventricular hypothalamic nucleus (PVN) by using an antisense probe. B: The same field of an adjacent section hybridized with a TGF-β1 control sense probe demonstrates no labeling. C: The distribution of TGF-β2 mRNA is shown in a photomicrograph of an area including parts of the cerebral cortex (Cx) by using an antisense probe. Labeling is intense in layer V. of the cortex, and also in the choroid plexus within the lateral ventricle. D: The same field of an adjacent section hybridized with a TGF-β2 control sense probe demonstrates no labeling. E: The distribution of TGF-β3 mRNA is shown in the anterior thalamus by using an antisense probe. TGF-β3 mRNA is present in the paraventricular thalamic nucleus (PVT), the reticular thalamic nucleus (Re), and the anterior thalamic nuclei including the anteromedial thalamic nucleus (AM). F: The same field of an adjacent section hybridized with a TGF-β3 control sense probe demonstrates no labeling. Additional abbreviations include fi: fimbria hippocampi, ic: internal capsule, ot: optic tract, sm: stria medullaris, and 3V: third ventricle. Scale bar = 1 mm.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.