Clathrin assembly proteins AP180 and CALM in the embryonic rat brain
Version of Record online: 20 MAY 2010
Copyright © 2010 Wiley-Liss, Inc.
Journal of Comparative Neurology
Volume 518, Issue 18, pages 3803–3818, 15 September 2010
How to Cite
Schwartz, C. M., Cheng, A., Mughal, M. R., Mattson, M. P. and Yao, P. J. (2010), Clathrin assembly proteins AP180 and CALM in the embryonic rat brain. J. Comp. Neurol., 518: 3803–3818. doi: 10.1002/cne.22425
- Issue online: 13 JUL 2010
- Version of Record online: 20 MAY 2010
- Accepted manuscript online: 20 MAY 2010 12:00AM EST
- Manuscript Accepted: 25 APR 2010
- Manuscript Revised: 2 APR 2010
- Manuscript Received: 21 JAN 2010
- Intramural Research Program of the National Institute on Aging
- MedStar Research Institute (R&D contract)
Additional Supporting Information may be found in the online version of this article.
|CNE_22425_sm_suppinfoFigureS1.tif||1243K||Supplementary Figure S1 RT-PCR analysis using different PCR primers showing that the expression of AP180 and CALM mRNAs in embryonic brains is similar to the patterns shown in Figure 1.|
|CNE_22425_sm_suppinfoFigureS2.tif||1513K||Supplementary Figure S2 Double immunostaining to sections of E14 brains as shown in the red boxed area in A. AP180 (green) and SOX2 (red) are expressed by mutually exclusive subsets of cells. E is a Z-plane view of D. The section was also stained by DAPI (blue). Scale bar: 10 μm.|
|CNE_22425_sm_suppinfoFigureS3.tif||1130K||Supplementary Figure S3 Double immunostaining of AP180 and TH in E14 ventral mesencephalon. A-C: A more lateral view (than in Figure 3C-E) of the ventral mesencephalon double stained for AP180 (green) and TH (red). D: An additional example of NT2 cells doubly stained with AP180 and TH. Scale bars: A, 20μm; D,10μm.|
|CNE_22425_sm_suppinfoFigureS4.tif||2508K||Supplementary Figure S4 Double immunostaining to sections of E14 brains using the same set of stage-specific markers but a CALM antibody (sc5395) different from the CALM antibody (sc6433) used in Figure 3. Scale bars: C, F, I, 10 μm; L, 5 μm.|
|CNE_22425_sm_suppinfoFigureS5.tif||3165K||Supplementary Figure S5 Double immunostaining of AP180 (green) and Tuj1 (red) in NTera2 cells differentiated for 2 weeks. Scale bar: 10μm.|
|CNE_22425_sm_suppinfoFigureS6.tif||2608K||Supplementary Figure S6 A, B: Graphs showing the percentage of E14 dissociated cortical cells expressing indicated markers. A, day 0; B, day 3. C-H: Sample images of vector-transfected cells (green) stained for Tuj1 (red). Scale bar: 20μm.|
|CNE_22425_sm_suppinfoTableS1.doc||34K||Supplementary Table 1 Primer sequences for RT-PCR|
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