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FilenameFormatSizeDescription
CNE_22427_sm_suppinfoFig2.tif9974KFig. 2S: Confocal microscopy of MOR (A, AA, E) or KOR (I, II, M) (red fluorescence) and VAChT (B, BB, F, J, JJ, N) (green fluorescence) double immunofluorescence in intracardiac ganglia (A-C, AA, CC, I-K, II-KK) or atrial myocardial tissue (E-G, M-O). A-C and I-K show the whole intracardiac ganglia with MOR and KOR co-expressed with VAChT in large diameter neurons (double arrow), however, some small diameter neurons expressed MOR or KOR only (arrow). AA-CC and II-KK show MOR and KOR co-expressed with VAChT in large diameter neurons. Note, VAChT immunoreactivity was also observed in pericellular boutons (double thin arrow) surrounding MOR- or KOR-IR cell bodies (double arrow) of most principal neurons and was generally more intense than within the cell bodies of neurons. E-G and M-O show MOR or KOR co-expressed with VAChT-IR nerve fibers (double arrows) between cardiomyocytes (M) in atrial myocardial tissue, however, some nerve fibres expressed VAChT (arrowhead) only. Bar=20 μm. A red-green fluorescence copy of this figure is available as figure 2.
CNE_22427_sm_suppinfoFig3.tif8964KFig. 3S: Confocal microscopy of MOR (A, E, I) or KOR (M, Q, T) (red fluorescence) and TH (B, F, J, N, R, U) (green fluorescence) double immunofluorescence in intracardiac ganglia (A-C, E-G, M-O-Q-S) or atrial myocardial tissue (I-K, T-V). M-O show KOR co-expressed with TH in small intensely fluorescent (SIF) cell but not in large-diameter neurons consistent with previous descriptions by McMahan and Purves (McMahan and Purves 1976) and Heym et al. (Heym et al., 1994) as intensely fluorescent (SIF) cells. A-C, E-G and Q-S are higher magnifications of intracardiac ganglia, showing a population of SIF cells co-expressing MOR or KOR with TH (double arrows), however, some SIF cells also expressed MOR, KOR (arrow) or TH (arrowhead) only. A-C and Q-S show TH-IR nerve fibers extending from SIF cells co-expressing MOR or KOR with TH (double thin arrows). I-K and T-V show MOR or KOR co-expressed with TH-IR nerve fibers (double arrows) between cradiomyocytes (M) in atrial myocardial tissue, however, some nerve fibres expressed TH (arrowhead) only. Bar=20 μm. A red-green fluorescence copy of this figure is available as figure 3.
CNE_22427_sm_suppinfoFig4.tif9816KFig. 4S: Confocal microscopy of MOR (A, E, I, Q) (red fluorescence) and CGRP (B, F, J) or SP (R) (green fluorescence) double immunofluorescence in intracardiac ganglia (A-C, I-K) or atrial myocardial tissue (E-G, Q-S). C, G, K, and S are combined images of MOR and CGRP or SP showing CGRP or SP co-expressed with MOR in nerve fibres (double arrows) but never in neuronal soma or SIF cells (arrow). Note, some nerve fibres expressed CGRP (arrowhead) only. I-K show CGRP-IR fibers co-expressing MOR formed pericellular complexes and came in close contact with MOR-IR neurons (K, L). Bar=20 μm. A red-green fluorescence copy of this figure is available as figure 4.
CNE_22427_sm_suppinfoFig5.tif9928KFig. 5S: Confocal microscopy of KOR (A, E, I, M) (red fluorescence) with CGRP (B, F) or SP (J, N) (green fluorescence) double immunofluorescence in intracardiac ganglia (A-C, I-K) or atrial tissue (E-G, M-O). C, G, K, and O are combined images of KOR and CGRP or SP showing CGRP or SP co-expressed with KOR in nerve fibres (double arrows), but never in neuronal soma or SIF cells (arrow). Note, some nerve fibres expressed CGRP (arrowhead) only. Bar=20 μm. A red-green fluorescence copy of this figure is available as figure 5.

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