The first two authors contributed equally to this study.
Astroglial loss and edema formation in the rat piriform cortex and hippocampus following pilocarpine-induced status epilepticus
Article first published online: 30 SEP 2010
Copyright © 2010 Wiley-Liss, Inc.
Journal of Comparative Neurology
Volume 518, Issue 22, pages 4612–4628, 15 November 2010
How to Cite
Kim, J.-E., Yeo, S.-I., Ryu, H. J., Kim, M.-J., Kim, D.-S., Jo, S.-M. and Kang, T.-C. (2010), Astroglial loss and edema formation in the rat piriform cortex and hippocampus following pilocarpine-induced status epilepticus. J. Comp. Neurol., 518: 4612–4628. doi: 10.1002/cne.22482
- Issue published online: 30 SEP 2010
- Article first published online: 30 SEP 2010
- Accepted manuscript online: 26 AUG 2010 03:03PM EST
- Manuscript Accepted: 23 JUL 2010
- Manuscript Revised: 2 MAR 2010
- Manuscript Received: 26 AUG 2009
- Korea Healthcare Technology R&D Project, Ministry for Health, Welfare and Family Affairs, Republic of Korea. Grant Number: A080007
- National Research Foundation of Korea. Grant Numbers: 2009-0064347, 2009-0093812
- piriform cortex;
In the present study we analyzed aquaporin-4 (AQP4) immunoreactivity in the piriform cortex (PC) and the hippocampus of pilocarpine-induced rat epilepsy model to elucidate the roles of AQP4 in brain edema following status epilepticus (SE). In non-SE-induced animals, AQP4 immunoreactivity was diffusely detected in the PC and the hippocampus. AQP4 immunoreactivity was mainly observed in the endfeet of astrocytes. Following SE the AQP4-deleted area was clearly detected in the PC, not in the hippocampus. Decreases in dystrophin and α-syntrophin immunoreactivities were followed by reduction in AQP4 immunoreactivity. These alterations were accompanied by the development of vasogenic edema and the astroglial loss in the PC. In addition, acetazolamide (an AQP4 inhibitor) treatment exacerbated vasogenic edema and astroglial loss both in the PC and in the hippocampus. These findings suggest that SE may induce impairments of astroglial AQP4 functions via disruption of the dystrophin/α-syntrophin complex that worsen vasogenic edema. Subsequently, vasogenic edema results in extensive astroglial loss that may aggravate vasogenic edema. J. Comp. Neurol. 518:4612–4628, 2010. © 2010 Wiley-Liss, Inc.