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Astroglial loss and edema formation in the rat piriform cortex and hippocampus following pilocarpine-induced status epilepticus

Authors

  • Ji-Eun Kim,

    1. Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chunchon, Kangwon-Do 200-702, South Korea
    2. Institute of Epilepsy Research, College of Medicine, Hallym University, Chunchon 200-702, South Korea
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    • The first two authors contributed equally to this study.

  • Seong-Il Yeo,

    1. Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chunchon, Kangwon-Do 200-702, South Korea
    2. Institute of Epilepsy Research, College of Medicine, Hallym University, Chunchon 200-702, South Korea
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    • The first two authors contributed equally to this study.

  • Hea Jin Ryu,

    1. Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chunchon, Kangwon-Do 200-702, South Korea
    2. Institute of Epilepsy Research, College of Medicine, Hallym University, Chunchon 200-702, South Korea
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  • Min-Ju Kim,

    1. Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chunchon, Kangwon-Do 200-702, South Korea
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  • Duk-Soo Kim,

    1. Department of Anatomy, College of Medicine, Soonchunhyang University, Cheonan, Chungcheongnam-Do 330-090, South Korea
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  • Seung-Mook Jo,

    1. Department of Anatomy, Gachon Medical Graduate School, Incheon 405-220, South Korea
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  • Tae-Cheon Kang

    Corresponding author
    1. Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chunchon, Kangwon-Do 200-702, South Korea
    2. Institute of Epilepsy Research, College of Medicine, Hallym University, Chunchon 200-702, South Korea
    • Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chunchon, Kangwon-Do 200-702, South Korea
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Abstract

In the present study we analyzed aquaporin-4 (AQP4) immunoreactivity in the piriform cortex (PC) and the hippocampus of pilocarpine-induced rat epilepsy model to elucidate the roles of AQP4 in brain edema following status epilepticus (SE). In non-SE-induced animals, AQP4 immunoreactivity was diffusely detected in the PC and the hippocampus. AQP4 immunoreactivity was mainly observed in the endfeet of astrocytes. Following SE the AQP4-deleted area was clearly detected in the PC, not in the hippocampus. Decreases in dystrophin and α-syntrophin immunoreactivities were followed by reduction in AQP4 immunoreactivity. These alterations were accompanied by the development of vasogenic edema and the astroglial loss in the PC. In addition, acetazolamide (an AQP4 inhibitor) treatment exacerbated vasogenic edema and astroglial loss both in the PC and in the hippocampus. These findings suggest that SE may induce impairments of astroglial AQP4 functions via disruption of the dystrophin/α-syntrophin complex that worsen vasogenic edema. Subsequently, vasogenic edema results in extensive astroglial loss that may aggravate vasogenic edema. J. Comp. Neurol. 518:4612–4628, 2010. © 2010 Wiley-Liss, Inc.

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