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Cellular composition and organization of the subventricular zone and rostral migratory stream in the adult and neonatal common marmoset brain

Authors

  • Kazunobu Sawamoto,

    Corresponding author
    1. Department of Developmental and Regenerative Biology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi 467-8601, Japan
    • Department of Developmental and Regenerative Biology, Nagoya City University Graduate School of Medical Sciences, 1-Kawasumi, Mizuho-ku, Mizuho-cho, Nagoya, Aichi 467-8601, Japan
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  • Yuki Hirota,

    1. Department of Developmental and Regenerative Biology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi 467-8601, Japan
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    • The first four authors contributed equally to this work.

  • Clara Alfaro-Cervello,

    1. Laboratorio de Neurobiología Comparada, Instituto Cavanilles, Universidad de Valencia, 46980, Valencia, Spain
    2. Laboratorio de Morfología Celular, Centro de Investigación Príncipe Felipe, CIBERNED, 46013, Valencia, Spain
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    • The first four authors contributed equally to this work.

  • Mario Soriano-Navarro,

    1. Laboratorio de Morfología Celular, Centro de Investigación Príncipe Felipe, CIBERNED, 46013, Valencia, Spain
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    • The first four authors contributed equally to this work.

  • Xiaoping He,

    1. Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan
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  • Yoshika Hayakawa-Yano,

    1. Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan
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  • Masayuki Yamada,

    1. Central Institute for Experimental Animals, Kawasaki, Kanagawa 216-0001, Japan
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  • Keigo Hikishima,

    1. Central Institute for Experimental Animals, Kawasaki, Kanagawa 216-0001, Japan
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  • Hidenori Tabata,

    1. Department of Anatomy, Keio University School of Medicine, Tokyo 160-8582, Japan
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  • Akio Iwanami,

    1. Department of Orthopedic Surgery, Keio University School of Medicine, Tokyo 160-8582, Japan
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  • Kazunori Nakajima,

    1. Department of Anatomy, Keio University School of Medicine, Tokyo 160-8582, Japan
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  • Yoshiaki Toyama,

    1. Department of Orthopedic Surgery, Keio University School of Medicine, Tokyo 160-8582, Japan
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  • Toshio Itoh,

    1. Central Institute for Experimental Animals, Kawasaki, Kanagawa 216-0001, Japan
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  • Arturo Alvarez-Buylla,

    1. Department of Neurological Surgery and Program in Developmental and Stem Cell Biology, University of California San Francisco, San Francisco, California 94143
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  • Jose Manuel Garcia-Verdugo,

    Corresponding author
    1. Laboratorio de Neurobiología Comparada, Instituto Cavanilles, Universidad de Valencia, 46980, Valencia, Spain
    2. Laboratorio de Morfología Celular, Centro de Investigación Príncipe Felipe, CIBERNED, 46013, Valencia, Spain
    • Laboratorio de Neurobiología Comparada, Instituto Cavanilles, Universidad de Valencia, 46980, Valencia, Spain
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  • Hideyuki Okano

    1. Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan
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Abstract

The adult subventricular zone (SVZ) of the lateral ventricle contains neural stem cells. In rodents, these cells generate neuroblasts that migrate as chains toward the olfactory bulb along the rostral migratory stream (RMS). The neural-stem-cell niche at the ventricular wall is conserved in various animal species, including primates. However, it is unclear how the SVZ and RMS organization in nonhuman primates relates to that of rodents and humans. Here we studied the SVZ and RMS of the adult and neonatal common marmoset (Callithrix jacchus), a New World primate used widely in neuroscience, by electron microscopy, and immunohistochemical detection of cell-type-specific markers. The marmoset SVZ contained cells similar to type B, C, and A cells of the rodent SVZ in their marker expression and morphology. The adult marmoset SVZ had a three-layer organization, as in the human brain, with ependymal, hypocellular, and astrocyte-ribbon layers. However, the hypocellular layer was very thin or absent in the adult-anterior and neonatal SVZ. Anti-PSA-NCAM staining of the anterior SVZ in whole-mount ventricular wall preparations of adult marmosets revealed an extensive network of elongated cell aggregates similar to the neuroblast chains in rodents. Time-lapse recordings of marmoset SVZ explants cultured in Matrigel showed the neuroblasts migrating in chains, like rodent type A cells. These results suggest that some features of neurogenesis and neuronal migration in the SVZ are common to marmosets, humans, and rodents. This basic description of the adult and neonatal marmoset SVZ will be useful for future studies on adult neurogenesis in primates. J. Comp. Neurol. 519:690–713, 2011. © 2010 Wiley-Liss, Inc.

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