A.J. Castiglioni and N.N. Remis contributed equally to this work.
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Research Article
Expression and vesicular localization of mouse Trpml3 in stria vascularis, hair cells, and vomeronasal and olfactory receptor neurons
Article first published online: 22 FEB 2011
DOI: 10.1002/cne.22554
Copyright © 2011 Wiley-Liss, Inc.
Additional Information
How to Cite
Castiglioni, A. J., Remis, N. N., Flores, E. N. and García-Añoveros, J. (2011), Expression and vesicular localization of mouse Trpml3 in stria vascularis, hair cells, and vomeronasal and olfactory receptor neurons. J. Comp. Neurol., 519: 1095–1114. doi: 10.1002/cne.22554
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A.J. Castiglioni and N.N. Remis contributed equally to this work.
Publication History
- Issue published online: 22 FEB 2011
- Article first published online: 22 FEB 2011
- Accepted manuscript online: 30 NOV 2010 09:13PM EST
- Manuscript Accepted: 4 NOV 2010
- Manuscript Revised: 7 OCT 2010
- Manuscript Received: 17 AUG 2010
Funded by
- National Institutes of Health. Grant Number: RO1 NS044363
- The Hugh Knowles Foundation
- National Research Service Award. Grant Numbers: NS041234, DC010529
Keywords:
- mucolipin;
- VNO;
- taste receptor;
- circumvallate;
- CVP;
- retina;
- lysosome;
- endosome;
- transient receptor potential;
- TRP;
- ion channel;
- Mcoln3
Abstract
TRPML3 is a member of the mucolipin branch of the transient receptor potential cation channel family. A dominant missense mutation in Trpml3 (also known as Mcoln3) causes deafness and vestibular impairment characterized by stereocilia disorganization, hair cell loss, and endocochlear potential reduction. Both marginal cells of the stria vascularis and hair cells express Trpml3 mRNA. Here we used in situ hybridization, quantitative RT-qPCR, and immunohistochemistry with several antisera raised against TRPML3 to determine the expression and subcellular distribution of TRPML3 in the inner ear as well as in other sensory organs. We also use Trpml3 knockout tissues to distinguish TRPML3-specific from nonspecific immunoreactivities. We find that TRPML3 localizes to vesicles of hair cells and strial marginal cells but not to stereociliary ankle links or pillar cells, which nonspecifically react with two antisera raised against TRPML3. Upon cochlear maturation, TRPML3 protein is redistributed to perinuclear vesicles of strial marginal cells and is augmented in inner hair cells vs. outer hair cells. Mouse somatosensory neurons, retinal neurons, and taste receptor cells do not appear to express physiologically relevant levels of TRPML3. Finally, we found that vomeronasal and olfactory sensory receptor cells do express TRPML3 mRNA and protein, which localizes to vesicles in their somas and dendrites as well as at apical dendritic knobs. J. Comp. Neurol. 519:1095–1114, 2011. © 2010 Wiley-Liss, Inc.