Neurochemical phenotypes of myenteric neurons in the rhesus monkey
Article first published online: 27 SEP 2011
Copyright © 2011 Wiley-Liss, Inc.
Journal of Comparative Neurology
Volume 519, Issue 17, pages 3387–3401, 1 December 2011
How to Cite
Noorian, A. R., Taylor, G. M., Annerino, D. M. and Greene, J. G. (2011), Neurochemical phenotypes of myenteric neurons in the rhesus monkey. J. Comp. Neurol., 519: 3387–3401. doi: 10.1002/cne.22679
- Issue published online: 27 SEP 2011
- Article first published online: 27 SEP 2011
- Accepted manuscript online: 25 MAY 2011 09:51AM EST
- Manuscript Accepted: 4 MAY 2011
- Manuscript Revised: 22 FEB 2010
- Manuscript Received: 26 OCT 2009
- National Institutes of Health (NIH). Grant Number: K08 NS048858
- Michael J. Fox Foundation for Parkinson's Research
Additional Supporting Information may be found in the online version of this article.
|CNE_22679_sm_suppfig1.tif||4792K||Supporting Figure 1 Assessment of myenteric neuron proportions (magenta-green depiction of text Figure 1). Relative proportions of myenteric neuron phenotypes were assessed using double-label immunofluorescence or combined immunohistochemistry/immunofluorescence. A. Myenteric ganglion from jejunum demonstrating double-label immunofluorescence for NOS and HuC/D. Double-labeled NOS-positive neurons (arrows) are white. NOS-negative neurons (HuC/D only) are magenta. NOS-positive processes are green. B. Myenteric ganglion from jejunum demonstrating ChAT immunohistochemistry. C. Fluorescent image of the same ganglion shown in B demonstrating quenched HuC/D fluorescence in the ChAT-positive neuron (arrow) and a ChAT-negative (HuC/D only) neuron in magenta. Scale bar = 20 μm.|
|CNE_22679_sm_suppfig2.tif||3623K||Supporting Figure 2 Co-expression of neurotransmitter markers in myenteric plexus in Rhesus monkey (magenta-green depiction of text Figure 5). A. Co-labeling for TH (magenta) and NOS (green) revealed minimal overlap in all GI segments examined. Section depicted is from duodenum. B. Co-labeling for TH (magenta) and VIP (green) revealed minimal overlap in all GI segments examined. Section depicted is from colon. C. Co-labeling for NOS (magenta) and VIP (green) revealed significant overlap (white) that varied by segment. Section depicted is from duodenum. Overlap in circular muscle processes is also prominent. Scale bar = 20 μm.|
|CNE_22679_sm_suppfig3.tif||2450K||Supporting Figure 3 Immunohistochemical labeling of a TH-positive neuron in monkey ileum myenteric plexus. TH-positive neuron identified by brown reaction product. TH-negative neurons (HuC/D only) are magenta.|
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