A-kinase anchoring protein 150 expression in a specific subset of TRPV1- and CaV1.2-positive nociceptive rat dorsal root ganglion neurons

Authors

  • Katherine E. Brandao,

    1. Program in Neuroscience, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, Colorado 80045
    2. Department of Physiology and Biophysics, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, Colorado 80045
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  • Mark L. Dell'Acqua,

    1. Program in Neuroscience, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, Colorado 80045
    2. Department of Pharmacology, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, Colorado 80045
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  • S. Rock Levinson

    Corresponding author
    1. Program in Neuroscience, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, Colorado 80045
    2. Department of Physiology and Biophysics, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, Colorado 80045
    • University of Colorado School of Medicine, Anschutz Medical Campus, 12800 E 19th Ave., P18-7103, Aurora, CO 80045
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Abstract

Modulation of phosphorylation states of ion channels is a critical step in the development of hyperalgesia during inflammation. Modulatory enhancement of channel activity may increase neuronal excitability and affect downstream targets such as gene transcription. The specificity required for such regulation of ion channels quickly occurs via targeting of protein kinases and phosphatases by the scaffolding A-kinase anchoring protein 79/150 (AKAP79/150). AKAP79/150 has been implicated in inflammatory pain by targeting protein kinase A (PKA) and protein kinase C (PKC) to the transient receptor potential vanilloid 1 (TRPV1) channel in peripheral sensory neurons, thus lowering threshold for activation of the channel by multiple inflammatory reagents. However, the expression pattern of AKAP150 in peripheral sensory neurons is unknown. Here we identify the peripheral neuron subtypes that express AKAP150, the subcellular distribution of AKAP150, and the potential target ion channels in rat dorsal root ganglion (DRG) slices. We found that AKAP150 is expressed predominantly in a subset of small DRG sensory neurons, where it is localized at the plasma membrane of the soma, axon initial segment, and small fibers. Most of these neurons are peripherin positive and produce C fibers, although a small portion produce Aδ fibers. Furthermore, we demonstrate that AKAP79/150 colocalizes with TRPV1 and CaV1.2 in the soma and axon initial segment. Thus AKAP150 is expressed in small, nociceptive DRG neurons, where it is targeted to membrane regions and where it may play a role in the modulation of ion channel phosphorylation states required for hyperalgesia. J. Comp. Neurol., 2012. © 2011 Wiley Periodicals, Inc.

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