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Additional Supporting Information may be found in the online version of this article.

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CNE_22692_sm_SuppFigure1.tif1244KSupplementary Figure 1: (This is a magenta-green version of Figure 3 for the assistance of color-blind readers.) Characterization of AKAP150 expression in myelinated and unmyelinated DRG neurons using confocal microscopy. Scale bars = 50μm. A-D. A subset of AKAP150 expressing DRG neurons coexpress Peripherin, a marker for unmyelinated neurons. AKAP150 (A), Peripherin (B) and overlay (C) AKAP150 (red) and Peripherin (green). A subset of AKAP150 expressing neurons coexpress Peripherin (white arrowhead, A-C), demonstrating that a subpopulation of AKAP150 expressing DRG neurons are small, unmyelinated c-fiber producing neurons, while another population of AKAP150 expressing neurons do not coexpress Peripherin (yellow arrow, A-C). D. AKAP150 expression and Peripherin expression is primarily seen in small DRG neurons. E-H. A small subset of AKAP150 positive DRG neurons coexpress NF-200, a marker for myelinated neurons. AKAP150 (E) and NF-200 (F) immunoreactivity and the overlay (G) of AKAP150 (red) and NF-200 (green) show that a subset of AKP150 expressing neurons produce myelinated fibers (E-F, white arrowhead), while the majority of AKAP150 positive neurons lack NF-200 immunoreactivity (yellow arrow). H. Cell size histograms demonstrate that AKAP150 expressed in small diameter DRG neurons while NF-200 is expressed in a broader range of neurons.
CNE_22692_sm_SuppFigure2.tif1606KSupplementary Figure 2: (This is a magenta-green version of Figure 4 for the assistance of color-blind readers.) AKAP150 is expressed in both peptidergic and non-peptidergic DRG neurons. Confocal microscopy, scale bars = 50μm. A-D. AKAP150 (A, C red) and IB4 (B, C green) immunoreactivity in rat DRG neurons shows that a subset of AKAP150 expressing neurons coexpress IB4 (A-C, white arrowhead) while another population only expressed AKAP150 (A-C, yellow arrow). The size distribution of AKAP150 or IB4 expressing neurons is similar and confined to small cell bodies (D). E-H. AKAP150 (E, G red) and CGRP (F, G green) immunoreactivity in rat DRG neurons shows that a subset of AKAP150 expressing neurons coexpresses CGRP (E-H, white arrowhead)) while another population only expressed AKAP150 (E-H, yellow arrow). The size distribution of AKAP150 or CGRP expressing neurons is similar and confined to small cell bodies (H). I-L. AKAP150 (I, K red) and SubP (J, K green) immunoreactivity in rat DRG neurons shows that only a very small subset of AKAP150 expressing neurons coexpress SubP (I-K, white arrowhead)) while the majority of AKAP150 positive neurons are SubP negative (I-K, yellow arrow). The size distribution of SubP expressing neurons was much broader than AKAP150 positive neurons (L).
CNE_22692_sm_SuppFigure3.tif1989KSupplementary Figure 3: (This is a magenta-green version of Figure 5 for the assistance of color-blind readers.) Characterization of AKAP150 expression with voltage gated ion channels and TRPV1 in rat DRG neurons. Confocal microscopy, scale bars = 50μm. A-D. AKAP150 (A, C red) highly coexpresses and colocalizes (C, yellow) with CaV1.2 (B, C green) in small DRG neurons. AKAP150 and CaV1.2 coexpressed in a subset of small DRG neurons (A-C, white arrowhead) while a smaller portion of neurons only expressed AKAP150 (A-C, yellow arrow). D. The majority of AKAP150 or CaV1.2 positive neurons were small, however this experiment showed the rare medium sized DRG neuron positive for AKAP150. E-H. AKAP150 immunoreactivity (E, G red) and VGSC immunoreactivity (F, G green) were seen together in a subset of DRG neurons (E-G, white arrowhead). Many AKAP150 positive neurons did not display VGSC immunoreactivity (E-G, yellow arrow). H. The cell size histogram shows the broader size distribution of VGSC positive neurons as compared to AKAP150 positive neurons. I-L. AKAP150 positive DRG neurons (I, K red) displayed levels of coexpression with KV1.2 (J, K green white arrowhead). The majority of AKAP150 positive neurons did not coexpress KV1.2 (I-K, yellow arrow). L. The cell size histogram shows the broad range of KV1.2 cell sizes while AKAP150 expressing neurons are primarily small. M-P. AKAP150 highly coexpresses with TRPV1. AKAP150 positive neurons (M, O red) coexpress TRPV1 (N, O green) in small DRG neurons (M-P, white arrowhead). A subpopulation of AKAP150 positive neurons does not coexpress TRPV1 (M-P, yellow arrow). P. The cell size histogram shows that AKAP150 and TRPV1 positive DRG neurons are confined to small cell sizes.
CNE_22692_sm_SuppFigure4.tif3009KSupplementary Figure 4: (This is a magenta-green version of Figure 6 for the assistance of color-blind readers.) Magnified confocal images of AKAP150 immunoreactivity in the membrane of DRG soma, axon initial segment and nerve fibers. Scale bars = 25μm. A-C. AKAP150 (A, C red) is expressed in the axon initial segment as indicated by colocalization with AnkG (B, C green) in the subcellular region (yellow arrow). D-F. AKAP150 (D, F red) does not colocalize with AnkG (E, F green) at the nodes of Ranvier (yellow arrow). G-I. AKAP150 immunoreactivity (G, I red) is not found at the nodes of Ranvier as identified by the paranodal marker caspr (H, I green, yellow arrow). J-L. AKAP150 (J, L red) is expressed in the axon initial segment of myelinated DRG neurons (yellow arrow) as identified by KV1.2 immunoreactivity (K, L red).
CNE_22692_sm_SuppFigure5.tif1298KSupplementary Figure 5: (This is a magenta-green version of Figure 7 for the assistance of color-blind readers.) Magnified confocal images demonstrating AKAP150 colocalization with CaV1.2 and TRPV1 in small, nociceptive DRG neurons. Scale bars = 25μm. A-C. AKAP150 (A, C red) and TRPV1 (B, C green) coexpress in small diameter DRG neurons and colocalize in the membrane of the soma (yellow arrow) and in the axon initial segment and nerve fiber (white arrowhead). D-F. AKAP150 (D, F red) and CaV1.2 (E, F green) coexpress in small DRG neurons and highly colocalize in the membrane of the soma (yellow arrow) and in the axon initial segment and fibers (white arrowhead).

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