Kisspeptin neurons co-express met-enkephalin and galanin in the rostral periventricular region of the female mouse hypothalamus
Article first published online: 27 SEP 2011
Copyright © 2011 Wiley-Liss, Inc.
Journal of Comparative Neurology
Volume 519, Issue 17, pages 3456–3469, 1 December 2011
How to Cite
Porteous, R., Petersen, S. L., Yeo, S. H., Bhattarai, J. P., Ciofi, P., D'anglemont de Tassigny, X., Colledge, W. H., Caraty, A. and Herbison, A. E. (2011), Kisspeptin neurons co-express met-enkephalin and galanin in the rostral periventricular region of the female mouse hypothalamus. J. Comp. Neurol., 519: 3456–3469. doi: 10.1002/cne.22716
- Issue published online: 27 SEP 2011
- Article first published online: 27 SEP 2011
- Accepted manuscript online: 28 JUL 2011 11:01AM EST
- Manuscript Accepted: 5 JUL 2011
- Manuscript Revised: 11 MAY 2011
- Manuscript Received: 27 AUG 2010
- National Institutes of Health. Grant Number: HD027305 (to S.L.P.)
- Health Research Council of New Zealand
It is now well established that the kisspeptin neurons of the hypothalamus play a key role in regulating the activity of gonadotropin-releasing hormone (GnRH) neurons. The population of kisspeptin neurons residing in the rostral periventricular region of the third ventricle (RP3V), encompassing the anteroventral periventricular (AVPV) and periventricular preoptic nuclei (PVpo), are implicated in the generation of the preovulatory GnRH surge mechanism and puberty onset in female rodents. The present study examined whether these kisspeptin neurons may express other neuropeptides in the adult female mouse. Initially, the distribution of galanin, neurotensin, met-enkephalin (mENK), and cholecystokinin (CCK)-immunoreactive cells was determined within the RP3V of colchicine-treated mice. Subsequent experiments, using a new kisspeptin-10 antibody raised in sheep, examined the relationship of these neuropeptides to kisspeptin neurons. No evidence was found for expression of neurotensin or CCK by RP3V kisspeptin neurons, but subpopulations of kisspeptin neurons were observed to express galanin and mENK. Dual-labeled RP3V kisspeptin/galanin cells represented 7% of all kisspeptin and 21% of all galanin neurons whereas dual-labeled kisspeptin/mENK cells represented 28–38% of kisspeptin neurons and 58–68% of the mENK population, depending on location within the AVPV or PVpo. Kisspeptin neurons in the arcuate nucleus were also found to express galanin but not mENK. These observations indicate that, like the kisspeptin population of the arcuate nucleus, kisspeptin neurons in the RP3V also co-express a range of neuropeptides. This pattern of co-expression should greatly increase the dynamic range with which kisspeptin neurons can modulate the activity of their afferent neurons. J. Comp. Neurol. 519:3456–3469, 2011. © 2011 Wiley-Liss, Inc.