Neurosensory and neuromuscular organization in tube feet of the sea urchin Strongylocentrotus purpuratus

Authors

  • Cavit Agca,

    1. Department of Ophthalmology, University of Zurich, Zurich, 8091 Switzerland
    2. Department of Biochemistry and Molecular Biology, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030
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  • Milad C. Elhajj,

    1. Department of Cell Biology and Anatomy, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112
    2. Department of Biology, Loyola University, New Orleans, Louisiana 70118
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  • William H. Klein,

    1. Department of Biochemistry and Molecular Biology, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030
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  • Judith M. Venuti

    Corresponding author
    1. Department of Cell Biology and Anatomy, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112
    • Biomedical Sciences, Oakland University William Beaumont School of Medicine, 527 O'Dowd Hall, 2200 North Squirrel Rd., Rochester, MI 48309-4401
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Abstract

Several behavioral and electrophysiological studies indicate that all classes of echinoderms, including Echinoidia, the class to which sea urchins belong, are photosensitive and exhibit complex behavioral responses to light or changes in light intensity. However, no discrete photosensitive structure has been identified in sea urchins. The purpose of this study was to provide new insights into eye evolution by determining whether distinct photosensory structures are present in adult sea urchins. Recently, we showed that the Strongylocentrotus purpuratus genome contains orthologs of many mammalian retinal genes and that these genes are expressed in tube feet, suggesting the presence of photoreceptor neurons. To determine whether this is so, we identified several features of tube feet that relate to a possible invertebrate phototransduction system. We show that rhabdomeric opsin is expressed severalfold higher within the disk region of the tube feet and is the most abundant opsin. Immunostaining identified βIII-tubulin-expressing cells at the periphery of disk in the vicinity of the synaptotagmin-expressing nerve fibers. We also showed that Pax6 expression in the disk was restricted to the periphery, where small clusters of putative sensory neurons reside. Our results reveal neuromuscular organization of the tube foot neuromuscular system. They further support earlier studies suggesting the presence of a photosensory system in tube feet. J. Comp. Neurol. 519:3566–3579, 2011. © 2011 Wiley-Liss, Inc.

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