Reelin demarcates a subset of pre-Bötzinger complex neurons in adult rat
Article first published online: 13 DEC 2011
Copyright © 2011 Wiley-Liss, Inc.
Journal of Comparative Neurology
Volume 520, Issue 3, pages 606–619, 15 February 2012
How to Cite
Tan, W., Sherman, D., Turesson, J., Shao, X. M., Janczewski, W. A. and Feldman, J. L. (2012), Reelin demarcates a subset of pre-Bötzinger complex neurons in adult rat. J. Comp. Neurol., 520: 606–619. doi: 10.1002/cne.22753
- Issue published online: 13 DEC 2011
- Article first published online: 13 DEC 2011
- Accepted manuscript online: 19 AUG 2011 09:59AM EST
- Manuscript Accepted: 12 AUG 2011
- Manuscript Revised: 11 JUN 2011
- Manuscript Received: 14 JAN 2011
- National Institutes of Health. Grant Number: HL40959
Identification of two markers of neurons in the pre-Bötzinger complex (pre-BötC), the neurokinin 1 receptor (NK1R) and somatostatin (Sst) peptide, has been of great utility in understanding the essential role of the pre-BötC in breathing. Recently, the transcription factor dbx1 was identified as a critical, but transient, determinant of glutamatergic pre-BötC neurons. Here, to identify additional markers, we constructed and screened a single-cell subtractive cDNA library from pre-BötC inspiratory neurons. We identified the glycoprotein reelin as a potentially useful marker, because it is expressed in distinct populations of pre-BötC and inspiratory bulbospinal ventral respiratory group (ibsVRG) neurons. Reelin ibsVRG neurons were larger (27.1 ± 3.8 μm in diameter) and located more caudally (>12.8 mm caudal to Bregma) than reelin pre-BötC neurons (15.5 ± 2.4 μm in diameter, <12.8 mm rostral to Bregma). Pre-BötC reelin neurons coexpress NK1R and Sst. Reelin neurons were also found in the parahypoglossal and dorsal parafacial regions, pontine respiratory group, and ventromedial medulla. Reelin-deficient (Reeler) mice exhibited impaired respones to hypoxia compared with littermate controls. We suggest that reelin is a useful molecular marker for pre-BötC neurons in adult rodents and may play a functional role in pre-BötC microcircuits. J. Comp. Neurol. 520:606–619, 2012. © 2011 Wiley Periodicals, Inc.