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Additional Supporting Information may be found in the online version of this article.

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CNE_22762_sm_SuppFigure1.tif1654KSupporting Figure 1. Photomicrographs of transverse sections through the telencephalon (a-e) and hypothalamus (f-t) of Pseudemys scripta, showing Nkx2.1 (a,f,k,p), Isl1(b,g,l,q), Otp (c,h,m,r), Pax6 (d,i,n,s), Tbr1 (e,j), Nkx2.2 (o) and Pax7 (t) immunostaining. Scale bar = 200 μm (valid for all photomicrographs). This figure corresponds to Supporting figure 1 in magenta-green version. This figure corresponds to the figure 3.
CNE_22762_sm_SuppFigure2.tif3218KSupporting Figure 2. Photomicrographs of transverse (a, b, e, h-jj) and sagittal (c,d,f and g) sections through the telencephalon of Pseudemys scripta, showing the combined expressions of Tbr1 and Isl1 (a), Nkx2.1 and Pax6 (b- e), Tbr1 and Pax6 (f,h) and Otp and Isl1 (g,i and j). The arrowheads in b, d and e indicate the Pax6 vz expression in the prethalamic eminence. The yellow lines in the sagittal sections presented in c, and g point to the level of the transverse sections indicated in each figure. The dashed box in c shows the magnified figure of d. Scale bar in a (valid for b,f,g and h) = 500μm, in c (valid for e, i and j) = 200 μm and in d = 100 μm. This figure corresponds to figure 4.
CNE_22762_sm_SuppFigure3.tif1665KSupporting Figure 3. Photomicrographs of transverse sections through the limit between the telencephalon and the hypothalamus of Pseudemys scripta, showing the combined expressions of Otp and Nkx2.1 (a), Otp and Nkx2.2 (b,c) and Nkx2.1 and Nkx2.2 (d,e). The asterisks in the panel show the preopto hypothalamic boundary. The dashed boxes in b and e show the magnified figure of c and e respectively. Scale bar in a and b = 200μm, in c and e =100 μm and in d = 500 μm. This figure corresponds to figure 5.
CNE_22762_sm_SuppFigure4.tif2646KSupporting Figure 4. Photomicrographs of transverse sections through the hypothalamus of Pseudemys scripta, showing the combined expressions of Otp and Isl1(a and i), Nkx2.1 and TH (b and c), Otp and TH (d-g), TH and Nkx2.2 (h), Tbr1 and Pax6 (j), Otp and Pax6 (k) andTbr1 and Isl1 (l). The dashed boxes in b and g show the magnified figure of c and h respectively. The yellow lines in the sagittal sections presented i point to the level of the transverse sections indicated. Scale bar in a (valid for b,d,f-l) = 500μm, in c (valid for e) = 200 μm. This figure corresponds to figure 6.
CNE_22762_sm_SuppFigure5.tif1725KSupporting Figure 5. Photomicrographs of transverse sections through the hypothalamus of Pseudemys scripta, showing the combined expressions of Otp and Isl1(a), Otp and TH (b), Otp and Pax6 (c), Pax6 and Nkx2.2 (d) and Otp and Nkx2.2 (e-g). The dashed box in f shows the magnified figure of g. Scale bar in a (valid for a-f) = 500μm, in g = 200 μm. This figure corresponds to the Supporting figure 5 in magenta-green version. This figure corresponds to figure 7.
CNE_22762_sm_SuppFigure6.tif3015KSupporting Figure 6. Photomicrographs of transverse (c,-e, g-j) and sagittal (a,b,f) sections through the hypothalamus of Pseudemys scripta, showing the combined expressions of Otp and TH (a-c), TH and Nkx2.2 (d), Nkx2.1 and TH (e), Otp and Isl1 (f), Nkx2.1 and Isl1 (g), Nkx2.1 and Nkx2.2 (h,i) and Otp and Nkx2.2 (j). The yellow line in the sagittal sections presented in a point to the level of the transverse sections indicated. The dashed box in a shows the magnified figure of b. Scale bar in a = 500μm, in e (valid for c,d,g,h and j) = 200 μm and in e (valid for f and i) = 100 μm. This figure corresponds to figure 7.
CNE_22762_sm_SuppFigure7.tif2263KSupporting Figure 7. Photomicrographs of transverse sections through the hypothalamus of Pseudemys scripta, showing the combined expressions of Nkx2.1 and Pax6 (a, f), Otp and Pax6 (b), Nkx2.1 and Isl1 (c-e) and Nkx2.1 (g). The figures h-j presented the same section with Isl1 (h), Otp (i) and the combination of both (j). The dashed box in d shows the magnified figure of i. Scale bar in a (valid for b,c,d and f) = 500μm, in e (valid for g-j) = 100 μm. This figure corresponds to figure 8.
CNE_22762_sm_SuppFigure8.tif3127KSupporting Figure 8. Photomicrographs of transverse sections through the hypothalamus of Pseudemys scripta, showing the combined expressions of Nkx2.1 and Isl1 (a, d), Nkx2.1 and TH (b, e), Nkx2.1 and Pax6 ©, Otp and Pax6 (f), Nkx2.1 and Nkx2.2 (g,i) and Nkx2.1 and Pax7 (h,j). Scale bar in a (valid for b,c,f,g) and = 500μm, in d (valid for i and j) = 200 μm and in e = 100 μm. This figure corresponds to figure 9.
CNE_22762_sm_SuppFigure9.tif2415KSupporting Figure 9. Photomicrographs of sagittal sections through the hypothalamus of Pseudemys scripta, showing the expressions of Nkx2.1 (a), Otp (b,h) and Nkx2.2 (o) and the combined expressions of Nkx2.1/Isl1 (c,d), Otp/Isl1 (e,f), Otp/TH (g,i,j), Otp/Pax7 (k,l), TH/Nkx2.2 (m,n). The dashed box in d, e, i and k show the magnified figure indicated in each figure. Scale bar in a (valid for b,c,e,g,k and m) = 500μm, in h (valid for d,f,i,n,o,l) = 200 μm and in j = 100 μm. This figure corresponds to figure 10.

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