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Additional Supporting Material may be found in the online version of this article.

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CNE_22808_suppinfofig1.tif3756KSupplementary Figure 1. (Magenta version of Figure 9 for the assistance of color-blind readers.) Expression of Pcdh1 (Pc1; C, D), Pcdh7 (Pc7; E, F), Pcdh8 (Pc8; G, H), Pcdh9 (Pc9; I, J) in motor neuron pools of the spinal cord at the lumbar level at 6 days' incubation (E6). Green color repesents mRNA signal of individual Pcdhs, as indicated. MNR2 (magenta color in A and J) and Islet1 (magenta color in B, D, F and H) serve as motor neurons markers (A, B). Hoechst 33258 staining visualizes nuclei (Hoe; blue color in A-C, E, G and I). The areas boxed in C, E, G and I are shown at a higher magnification in D, F, H and J, respectively. The dashed lines in A, B, D, F, H and J outline the different motor neuron pools. All panels are merged images for different Pcdhs, Islet1 or MNR2, and Hoechst staining, respectively (as indicated in each panel). Abbreviation: drg, dorsal root ganglia; lm, lateral motor column; lml, lateral lm; lmm, medial lm; mm, medial motor column. Scale bars: 100μm in D for F, H and J; 200μm in A for B, C, E, G and I. The same figure is shown in Figure 9 with magenta color replaced by red color.
CNE_22808_suppinfofig2.tif3867KSupplementary Figure 2. A-F. (Magenta version of Figure 10 for the assistance of color-blind readers.) Expression of Pcdh10 (Pc10; A, B), Pcdh17 (Pc17; C, D) and Pcdh19 (Pc19; E, F) in motor neuron pools of the spinal cord at the lumbar level at 6 days' incubation (E6). Green color repesents mRNA signal of individual Pcdhs, as indicated. MNR2 (magenta color in D and F) and Islet1 (magenta color in B) serve as motor neurons markers. Hoechst 33258 staining visualizes nuclei (Hoe; blue color in A, C and E). The areas boxed in A, C and E are shown at a higher magnification in B, D and F, respectively. The dashed lines in B, D and F outline the different motor neuron pools. The panels are merged images for different Pcdhs, and Islet1 or MNR2, and Hoechst staining, respectively (as indicated in each panel). The asterisks indicate artificial folds in the tissue in C, and a bubble artefact beneath the section in E. Abbreviation: drg, dorsal root ganglia; lm, lateral motor column; lml, lateral lm; lmm, medial lm; mm, medial motor column. Scale bar: 100μm in B for D and F; 200μm in A for C and E. G-N. Expression of Pcdh1 (Pc1; G-J) and Pcdh19 (Pc19; K-N) in blood vessels of the spinal cord at E10. In situ hybridization results for Pcdh1 (G) and Pcdh19 (K) shows their expression in blood vessels. Immunostaining using an antibody against alpha smooth muscle actin (SMA; magenta color in I, J, M and N) serves as a marker of pericytes. Hoechst 33258 staining visualizes nuclei (Hoe; blue color in A, C and E). H-J and L-N are merged images for SMA, Pcdh1 or Pcdh19, and Hoechst staining, respectively (as indicated in each panel). Scale bar: 40μm in G for H-N. The same figure is shown in Figure 10 with magenta color replaced by red color in panels A-F.
CNE_22808_suppinfofig3.tif3877KSupplementary Figure 3. A-L. (Magenta version of Figure 10 for the assistance of color-blind readers.) Computer-generated overlay of different delta-Pcdhs and dorsoventral domain markers Cdh7 and Pax3 at embryonic incubation day 4 (E4) at the lumbar level. M-O. Schematic diagrams of the expression patterns for delta-Pcdhs at E4 (M), E6 (N) and E10 (O), respectively. The arrow in A points to the expression boundary between Cdh7 and Pax3, which coincides with the alar/basal plate boundary. The delta-Pcdhs are represented by different colors, as indicated. Abbreviations: dh, dorsal horn; dr, dorsal root; drg, dorsal root ganglion; fp, floor plate; gm, gray matter; lm, lateral motor neuron column; mc, motor column; me, meninges; ml, mantle layer; mm, medial motor neuron column; mt, myotome; nc, notochord; ncc, neural crest cell; ne, neuroepithelial layer; rp, roof plate; sc, sclerotome; sg, sympathetic ganglion; sn, spinal nerve; sp, spinal cord; ve, vertebrae; vh, ventral horn; wm, white matter. Scale bar: 50μm in A for B-L; 100μm in M-O. The same figure is shown in Figure 14 with magenta color replaced by red color in panels A-L.

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