Cyto- and chemoarchitecture of the hypothalamic paraventricular nucleus in the C57BL/6J male mouse: A study of immunostaining and multiple fluorescent tract tracing

Authors

  • Jonathan Biag,

    1. Laboratory of Neuroimaging, Department of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095-7334
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  • Yi Huang,

    1. Laboratory of Neuroimaging, Department of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095-7334
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  • Lin Gou,

    1. Laboratory of Neuroimaging, Department of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095-7334
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  • Houri Hintiryan,

    1. Laboratory of Neuroimaging, Department of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095-7334
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  • Asal Askarinam,

    1. Laboratory of Neuroimaging, Department of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095-7334
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  • Joel D. Hahn,

    1. Brain Architecture Center, University of Southern California, Los Angeles, California 90089-2520
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  • Arthur W. Toga,

    1. Laboratory of Neuroimaging, Department of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095-7334
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  • Hong-Wei Dong

    Corresponding author
    1. Laboratory of Neuroimaging, Department of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095-7334
    2. Brain Research Institute, David Geffen School of Medicine at UCLA, Los Angeles, California 90095-7334
    • David Geffen School of Medicine at UCLA, 635 Charles E. Young Drive South, Suite 225, Los Angeles, CA 90095-7334
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Abstract

A composite confocal photomicrograph showing the organization an topographic relations of specific neuronal populations in the hypothalamic paraventricular nucleus (PVH) of a C57BL/6malemouse. One population of PVH neurons (presumptive neuroendocrine) was identified by immunocytochemical detection of the retrograde tracer Fluorogold (FG; green) following its injection in to the tail vein; a second population of PVH neurons (presumptive preautonomic) was back-labeled following upper thoracic level spinal cord injection of the retrograde tracer Fast Blue (FB; blue). Subsequently, distinct populations of oxytocin (OXY; red)- and vasopressin (VAS; magenta)- expressing neurons were identified immunocytochemically. At this PVH level neurons labeled only with FG (green) include numerous presumptive parvicellular neuroendocrine neurons. None of the FB (blue) back-labeled neurons were immunopositive for OXY or VAS at this PVH level; however all of the OXY- and VAS immunopositive neurons were back-labeled with FG, although the labeling intensity varied. Thus, OXY-expressing FG labeled neurons appear red/yellow, whereas VAS-expressing FG labeled neurons appear magenta/white. The Journal of Comparative Neurology, Volume 520, Number 1, pages 6–33.

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