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Electron tomographic analysis of synaptic ultrastructure

Authors

  • Alain C. Burette,

    Corresponding author
    1. Department of Cell & Developmental Biology, University of North Carolina, Chapel Hill, North Carolina, 27599
    • Department of Cell & Developmental Biology, University of North Carolina, CB # 7090, Chapel Hill, NC 27599
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  • Thomas Lesperance,

    1. Sanford-Burnham Medical Research Institute, La Jolla, California, 92093
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  • John Crum,

    1. Department of Neurosciences and National Center for Microscopy and Imaging Research, Center for Research in Biological Systems, University of California, San Diego, La Jolla, California, 92093
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  • Maryann Martone,

    1. Department of Neurosciences and National Center for Microscopy and Imaging Research, Center for Research in Biological Systems, University of California, San Diego, La Jolla, California, 92093
    2. Department of Neurosciences, University of California, San Diego, La Jolla, California, 92093
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  • Niels Volkmann,

    1. Sanford-Burnham Medical Research Institute, La Jolla, California, 92093
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  • Mark H. Ellisman,

    1. Department of Neurosciences and National Center for Microscopy and Imaging Research, Center for Research in Biological Systems, University of California, San Diego, La Jolla, California, 92093
    2. Department of Neurosciences, University of California, San Diego, La Jolla, California, 92093
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  • Richard J. Weinberg

    1. Department of Cell & Developmental Biology, University of North Carolina, Chapel Hill, North Carolina, 27599
    2. Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina, 27599
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Abstract

Synaptic function depends on interactions among sets of proteins that assemble into complex supramolecular machines. Molecular biology, electrophysiology, and live-cell imaging studies have provided tantalizing glimpses into the inner workings of the synapse, but fundamental questions remain regarding the functional organization of these “nano-machines.” Electron tomography reveals the internal structure of synapses in three dimensions with exceptional spatial resolution. Here we report results from an electron tomographic study of axospinous synapses in neocortex and hippocampus of the adult rat, based on aldehyde-fixed material stabilized with tannic acid in lieu of postfixation with osmium tetroxide. Our results provide a new window into the structural basis of excitatory synaptic processing in the mammalian brain. J. Comp. Neurol. 520:2697–2711, 2012. © 2012 Wiley Periodicals, Inc.

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