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Additional Supporting Information may be found in the online version of this article.

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CNE_23068_sm_SuppFig1.tif12116KSupporting Information Figure 1. (magenta-green version of Figure 2 from the manuscript for the assistance of color blind readers): Classification of cb3a and cb3b Off bipolar cells. A-C. Section was labeled with antibodies to RCV (green) and SCG (red). A. RCV positive cells are bistratified. RCV also labels the cone terminals at the top of the section. B. SCG strongly labels cells with axons that ramify in sublamina b and more weakly labels the somata and axon terminals of cells that ramify in sublamina a. C. The SCG positive bipolar cells that ramify in sublamina a are either RCV positive (yellow) and thus cb3b cells or RCV negative and thus cb3a cells (24 sections×0.37 μm thickness each). C, inset. An enlarged view of cb3a and cb3b somata and processes in the area delimited by the dashed box. D. The top cb3b cell stratum rests on top of a ChAT-positive band (22×1.0 μm). E. cb3a (right) and cb3b (left) cells were injected (INJ) with Neurobiotin tracer via whole cell patch pipettes. The soma of the cb3b cell is RCV positive (20×1.0 μm). F. cb3a (right) and cb3b (left) cells labeled with GFP following transduction by AAV2 (35×0.76 μm). G. A cb3a cell was labeled with Neurobiotin tracer following intracellular recording. The cell has a small lateral process (arrow) at the same level as the upper stratification of cb3b cells (15×0.4 μm). Scale bars equal 10 μm. The approximate distal and proximal borders of the IPL are marked at the side of panels with solid lines. The approximate location of the sublamina a/b border is shown by a dashed line.
CNE_23068_sm_SuppFig2.tif9247KSupporting Information Figure 2. (magenta-green version of Figure 4 from the manuscript for the assistance of color blind readers): Classification of cb1a and cb1b cells. A. An antibody to CaBP5 faintly labeled a band of processes at the top of the IPL (asterisk). The axons that extend into this band originated in lightly labeled somata. B. An antibody to PKA-RII? (PKA) labels somata in the INL and processes in several layers of the IPL. C. The same section with PKA and CaBP5 labeling superimposed. Some lightly-labeled CaBP5 positive somata are PKA positive. Somata can be tentatively divided into two types based on their labeling patterns. One type (arrows) typically has a crescent of PKA positive cytoplasm around the nucleus, whereas in the other type (arrow heads), PKA and CaBP5 labeling overlap near the axonal pole (18×0.38 μm). D. Three bipolar cells were injected with tracer and then the section was labeled with an antibody to RCV (27×1.0 μm). E. The two bipolar cells on the right were reconstructed from individual confocal sections. Their axonal processes have different patterns of ramification and extensively overlap. F. A superimposed DIC image from the same set of confocal sections shows that the terminals of both cell types arborize at the top of sublamina a. G. An example of adjacent cb1a and cb1b cells from AAV2 labeled tissue (54×0.5 μm). H. A GFP labeled cb1a cell was also labeled by an antibody to CaBP5 (3×1.1 μm). Scale bars equal 10 μm.
CNE_23068_sm_SuppFig3.tif6258KSupporting Information Figure 3. (magenta-green version of Figure 6 from the manuscript for the assistance of color blind readers): Classification of cb5a and cb5b cells. A. Vertical section from a retina labeled with antibodies to calb, PKC?, and ChAT (19×0.5 μm). Calb positive axons ramify just above the lower ChAT band and come in two types: PKC? positive (yellow, cb5b) and negative (red, cb5a). B. An enlarged section from A., annotated according to cell type and with ChAT labeling removed (rb, rod bipolar cell). C. A collapsed stack of horizontal sections showing the ramifications of calb and PKC? positive cb5b and calb positive cb5a cells (14×0.7 μm). Scale bars equal 10 μm.
CNE_23068_sm_SuppFig4.tif11747KSupporting Information Figure 4. (magenta-green version of Figure 8 from the manuscript for the assistance of color blind readers): Classification of cb6a and cb6b cells. A-C. Retinal slices containing cb6a and cb6b type cells labeled with GFP. A. GFP labels two similar cb6b bipolar cells which have a lower stratification that spans the lower ChAT band and an upper stratification that overlaps with a central ChAT band (96×0.36 μm). B. Two cb6 cells, one (left side) with a bistratified cb6b-type morphology and the other (right side) with more narrowly multistratifying terminals (cb6a type). The top level of the cb6a cell ramification starts just below the central ChAT band and ends just within the upper border of the lower ChAT band (43×0.8 μm). C. Two GFP labeled cb6a bipolar cells (38×0.8 μm). D. A cb6a bipolar cell was filled with tracer during recording, fixed, and than labeled with an antibody to PKC?. The filled bipolar cell was PKC? positive (23×0.45 μm). E, F. A GFP labeled cb6b type bipolar cell which has processes that ramify in two strata. PKC? co-labeling is minimal within the area delimited by the cell border. Faint PKC? labeling near the top of the IPL is contributed by amacrine cells whose somata are visible just above the INL/IPL border (14×1.1 μm). G. cb6b (INJ) and cb3b (RCV) positive cells are symmetrically organized relative to the sublamina a/b border (34×1.1 μm). Scale bars equal 10 μm.

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