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CNE_23097_sm_SuppFig1.tif6162KSupporting Information Figure 1. Copy of Figure 13 for the assistance of color-blind readers showing photomicrographs of immunohistochemical-stained transverse sections through the telencephalon of A. leptorhynchus converted to magenta-green fluorescence in Photoshop. A: GABA-immunoreactive (green) transverse sections counterstained with fluorescent Nissl (magenta) through the intermediate subdivision (DDi) of the dorsal division of dorsal telencephalon (DD). Co-localization of GABAergic cells with Nissl stained cells (white) reveals a small number of GABAergic cells when compared to the total number of cells in the nucleus. B: GABA-immunoreactive (green) transverse sections counterstained with fluorescent Nissl (magenta) through the magnocellular subdivision of DD (DDmg). Note that the scattered small GABA-immunoreactive neurons do not co-localize with the Nissl-stained large cells (arrows). Note GABA staining of small puncta (and a few fine fibers) in DDmg and in the dorsolateral (DL) telencephalon; these are assumed to be GABAergic boutons corresponding to the densely stained neuropil seen with the GAD antibody. Also note lack of such staining in the dorsocentral telencephalon (DC). C: Double-labeled transverse sections through DL. GABA-immunoreactive neurons (green) do not co-localize with CaMKIIa-immunoreactive cells (magenta, arrows) or neuropil. Scale bars = 50μm in A, C; 100μm in B.

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