Developmental dynamics of PAFAH1B subunits during mouse brain development

Authors

  • Teresa Escamez,

    1. Unidad Mixta de Investigación UVEG-UMH-CIBERSAM, Centro de Investigación Biomédica en Red en el Area de Salud Mental (CIBERSAM), 03550 San Juan de Alicante, Spain
    2. Biobanco En Red De La Región De Murcia (BIOBANC-Mur-Nodo Arrixaca), Instituto Murciano de Investigación Biosanitaria (IMIB), Fundación para la Formación e Investigación Sanitarias de la Región de Murcia (FFIS), 30008 Murcia, Spain
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  • Olga Bahamonde,

    1. Instituto de Neurociencias de Alicante, Universidad Miguel Hernández-Consejo Superior de Investigaciones Científicas, 03540 San Juan de Alicante, Spain
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  • Rafael Tabares-Seisdedos,

    1. Unidad Mixta de Investigación UVEG-UMH-CIBERSAM, Centro de Investigación Biomédica en Red en el Area de Salud Mental (CIBERSAM), 03550 San Juan de Alicante, Spain
    2. Department of Medicine, University of Valencia, Centro de Investigación Biomédica en Red en el Area de Salud Mental (CIBERSAM), 46003 Valencia, Spain
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  • Eduard Vieta,

    1. Bipolar Disorders Program, Hospital Clinic, University of Barcelona, Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS), Centro de Investigación Biomédica en Red en el Area de Salud Mental (CIBERSAM), 08036 Barcelona, Spain
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  • Salvador Martinez,

    1. Instituto de Neurociencias de Alicante, Universidad Miguel Hernández-Consejo Superior de Investigaciones Científicas, 03540 San Juan de Alicante, Spain
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  • Diego Echevarria

    Corresponding author
    1. Instituto de Neurociencias de Alicante, Universidad Miguel Hernández-Consejo Superior de Investigaciones Científicas, 03540 San Juan de Alicante, Spain
    • Instituto de Neurociencias de Alicante (UMH-CSIC), Universidad Miguel Hernández de Elche, Avda Ramón y Cajal s/n, Campus de San Juan 03550 San Juan de Alicante, Spain
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Abstract

Platelet-activating factor (PAF) mediates an array of biological processes in the mammalian central nervous system as a bioactive lipid messenger in synaptic function and dysfunction (plasticity, memory, and neurodegeneration). The intracellular enzyme that deacetylates the PAF (PAFAH1B) is composed of a tetramer of two catalytic subunits, ALPHA1 (PAFAH1B3) and ALPHA2 (PAFAH1B2), and a regulatory dimer of LIS1 (PAFAH1B1). We have investigated the mouse PAFAH1B subunit genes during brain development in normal mice and in mice with a hypomorphic allele for Lis1 (Lis1/sLis1; Cahana et al. [2001] Proc Natl Acad Sci U S A 98:6429–6434). We have analyzed quantitatively (by means of real-time polymerase chain reaction) and qualitatively (by in situ hybridization techniques) the amounts and expression patterns of their transcription in developing and postnatal brain, focusing mainly on differences in two laminated encephalic regions, the forebrain (telencephalon) and hindbrain (cerebellum) separately. The results revealed significant differences in cDNA content between these two brain subdivisions but, more importantly, between the LIS1 complex subunits. In addition, we found significant spatial differences in gene expression patterns. Comparison of results obtained with Lis1/sLis1 analysis also revealed significant temporal and spatial differences in Alpha1 and Lis1 expression levels. Thus, small changes in the amount of the Lis1 gene may differentially regulate expression of Alpha1 and Alpha2, depending on the brain region, which suggests different roles for each LIS1 complex subunit during neural differentiation and neural migration. J. Comp. Neurol. 520:3877–3894, 2012. © 2012 Wiley Periodicals, Inc.

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