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Additional Supporting Information may be found in the online version of this article.

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CNE_23163_sm_SuppFig1.tif7875KSupporting Figure 1 (Magentahyphen;green version of Figure 2 for the assistance of colorhyphen;blind readers): Photomicrographs of doublehyphen;labeled CB/CR sagittal (a, dorsal is upwards and rostral to the left) and transverse (bhyphen;t) sections through representative levels of the brain of Xenopus at embryonic stages (indicated on each photomicrograph).The levels of transverse sections are indicated in Figure 1a and b. In all photographs green is CB and red is CR, whereas yellow represents double labeled cells. Dashed lines mark the approximate boundary between neuromeres and telencephalic subdivisions. Arrowheads in f point to the lateral CRir fiber tract in p2, in h and s point to the lateral CRir fiber tract in the basal plate, and in q to doble labeled cells in the LDT. The asterisks in r and t mark the CBir fibers in the lateral rhombencephalon. For abbreviations, see list. A magentahyphen;green version of this figure is provided as Supporting Information Figure 1. Scale bar = 50 μm in ahyphen;h; 100 μm in ihyphen;t.
CNE_23163_sm_SuppFig2.tif8244KSupporting Figure 2 (Magentahyphen;green version of Figure 3 for the assistance of colorhyphen;blind readers): Photomicrographs of doublehyphen;labeled CB/CR sagittal (a,k,l; dorsal is upwards and rostral to the left) and transverse (bhyphen;j) sections through representative levels of the brain of Xenopus at premetamorphic stages (indicated on each photomicrograph). The levels of transverse sections for stage 50, and the area shown in a, are indicated in Figure 1c. In all photographs green is CB and red is CR, whereas yellow represents double labeled cells. Dashed lines represent the approximate boundary between telencephalic (e,f) or diencephalic (a,b,h,k,l) subdivisions, the mesencephalic longitudinal bands (c,g,k) and rhombencephalic regions (d,i,j). For abbreviations, see list. A magentahyphen;green version of this figure is provided as Supporting Information Figure 2. Scale bar =100 μm in ahyphen;j,l; 200 μm in k.
CNE_23163_sm_SuppFig3.tif8309KSupporting Figure 3 (Magentahyphen;green version of Figure 4 for the assistance of colorhyphen;blind readers): Photomicrographs of doublehyphen;labeled CB/CR transverse (ahyphen;h) and sagittal (ihyphen;k; dorsal is upwards and rostral to the left) sections through representative levels of the brain of Xenopus at prometamorphic stages (indicated on each photomicrograph). The levels of transverse sections are indicated in Figure 1d. In all photographs green is CB and red is CR, whereas yellow represents double labeled cells. Dashed lines represent the approximate boundary between telencephalic (ahyphen;d, i), diencephalic (ehyphen;g, ihyphen;k), mesencephalic (g,h) and rhombencephalic (i) subdivisions. i: Low magnification image of a lateral sagittal section showing the relationship between CB and CR labeled structures throughout the brain of a prometamorphic larva. j,k: Details of sagittal sections through the hypothalamic (j) and pretectal (k) region showing the relative localization of CBhyphen; and CRhyphen;labeled structures in each subdivision.. Inset in panel g' shows trigeminal mesencephalic neurons (arrowheads) in the rostral optic tectum. For abbreviations, see list. A magentahyphen;green version of this figure is provided as Supporting Information Figure 3. Scale bar =100 μm in ahyphen;h,k; 200 μm in i,j.
CNE_23163_sm_SuppFig4.tif8534KSupporting Figure 4 (Magentahyphen;green version of Figure 5 for the assistance of colorhyphen;blind readers): Photomicrographs of doublehyphen;labeled sagittal (ahyphen;i; dorsal is upwards and rostral to the left) and horizontal (j; rostral is to the left) sections through the brain of Xenopus at different developmental stages showing the distribution of CB or CR immunoreactive cells with nitric oxide synthase (NOS), tyrosine hydroxylase (TH) or choline acelyltransferase (ChAT). The markers shown in each panel are indicated in aprópiate color code, and the developmental stage is indicated in the upper rigth corner. ahyphen;c: relative localization of nitrergic (a) catecholaminergic (b) and cholinergic (c) populations compared to CR at stage 45. d: Colocalization of CR in the large niterergic cells of the central amygdala (double labeled cells in yellow). e: Distribution of CR in relation to the catecholaminergic cells in the preoptic and suprachiasmatic regions. f: Segmental distribution of the alar CRir cell groups in relation to the basal catecholaminergic cell groups, g: CRir and NOSir cells in the distinct dorsal and lateral bands in the mesencephalon, and along the rhombencephalon. h: High magnification of double CR/NOS labeled cells in the inferior reticular nucleus. i: CBir cells in the alar region of r0 and r1 in relation to the cholinergic nuclei. Arrowhead points to the fibers of the troclear nucleus in the basal plate (not shown). j: Horizontal section showing the relative position and the segmental arrangement of CR groups in relation to the ChATir motor and nonhyphen;motor cholinergic groups in the rhombencephalon. khyphen;m: Relative localization CBir cells in relation to the oculomotor, isthmic, rhombencephalic and spinal cholinergic cell groups. Doublehyphen;labeled cells always appear in yellow. nhyphen;p: CB and NOShyphen;stained structures in a premetamorphic larva in the mesencephalon and rostral rhombencephalon (n) and in prometamorphic larvae in the caudal telencephalon (o) and mesencephalon and rostral rhombencephalon (p). Double labeled CB/NOSir cells (in yellow) in the basal band of the mesencephalon and in the LDT at stage 50 were not observed at stage 60. For abbreviations, see list. Arrows point to doble labeled cells. A magentahyphen;green version of this figure is provided as Supporting Information Figure 4. Scale bar = 50 μm in a,b,dhyphen;f,h, khyphen;p; 100 μm in c,g, i,j.

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