The first five authors contributed equally to this work.
Transgenic quail as a model for research in the avian nervous system: A comparative study of the auditory brainstem
Article first published online: 22 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Comparative Neurology
Volume 521, Issue 1, pages 5–23, 1 January 2013
How to Cite
Seidl, A. H., Sanchez, J. T., Schecterson, L., Tabor, K. M., Wang, Y., Kashima, D. T., Poynter, G., Huss, D., Fraser, S. E., Lansford, R. and Rubel, E. W. (2013), Transgenic quail as a model for research in the avian nervous system: A comparative study of the auditory brainstem. J. Comp. Neurol., 521: 5–23. doi: 10.1002/cne.23187
- Issue published online: 22 NOV 2012
- Article first published online: 22 NOV 2012
- Accepted manuscript online: 14 JUL 2012 01:49AM EST
- Manuscript Accepted: 6 JUL 2012
- Manuscript Revised: 26 JUN 2012
- Manuscript Received: 25 APR 2012
- National Institute on Deafness and Other Communication Disorders, U.S. Public Health Service. Grant Number: R01 DC03829 and Research Core Center DC04661
- National Center for Research Resources, National Institutes of Health. Grant Number: R21HD047347-01
- Center of Excellence in Genomic Science, National Institutes of Health. Grant Number: P50 HG004071
- National Institute of Dental and Craniofacial Research. Grant Number: FaceBase U01 DE020063
- transgenic quail;
- auditory brainstem
Research performed on transgenic animals has led to numerous advances in biological research. However, using traditional retroviral methods to generate transgenic avian research models has proved problematic. As a result, experiments aimed at genetic manipulations on birds have remained difficult for this popular research tool. Recently, lentiviral methods have allowed the production of transgenic birds, including a transgenic Japanese quail (Coturnix coturnix japonica) line showing neuronal specificity and stable expression of enhanced green fluorescent protein (eGFP) across generations (termed here GFP quail). To test whether the GFP quail may serve as a viable alternative to the popular chicken model system, with the additional benefit of genetic manipulation, we compared the development, organization, structure, and function of a specific neuronal circuit in chicken (Gallus gallus domesticus) with that of the GFP quail. This study focuses on a well-defined avian brain region, the principal nuclei of the sound localization circuit in the auditory brainstem, nucleus magnocellularis (NM), and nucleus laminaris (NL). Our results demonstrate that structural and functional properties of NM and NL neurons in the GFP quail, as well as their dynamic properties in response to changes in the environment, are nearly identical to those in chickens. These similarities demonstrate that the GFP quail, as well as other transgenic quail lines, can serve as an attractive avian model system, with the advantage of being able to build on the wealth of information already available from the chicken. J. Comp. Neurol.5–23, 2013. © 2012 Wiley Periodicals, Inc.