SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
CNE_23273_sm_SuppFig1.tif7396KSupporting Information Figure 1. Characterization of MCHR1-expressing neurons in opioidergic neurons within the paraventricular nucleus of the hypothalamus. Representative photomicrographs of two-color confocal images showing that a subpopulation of tdTomato fluorescent neurons in the paraventricular nucleus express immunoreactivity for A-C, dynorphin-A; D-F, enkephalin. Singly-labeled neurons appear either magenta or green while coexpression (arrowhead) is indicated by cells appearing white due to color overlay in the merged image (C, F). All stainings were performed in Mchr1-cre/tdTomato mouse brains. Inset within merged image panels C, F indicate the relative position of the hypothalamic region imaged. Only a representative sample of coexpressing cells from each panel was marked. Scale bar, 50 μm. Corresponding red-green images are provided in Figure 10.
CNE_23273_sm_SuppFig2.tif14583KSupporting Information Figure 2. Characterization of MCHR1-expressing parvocellular and magnocellular neurons in the paraventricular nucleus of the hypothalamus. Representative photomicrographs of two-color confocal images showing that a subpopulation of tdTomato fluorescent neurons in the paraventricular nucleus express immunoreactivity for A-C, corticotropin-releasing factor; D-F, thyrothropin-releasing hormone; G-I, Arg-vasopressin; J-L, oxytocin. Singly-labeled neurons appear either magenta or green while coexpression (arrowhead) is indicated by cells appearing white due to color overlay in the merged image (C, F, I, L). All stainings were performed in Mchr1-cre/tdTomato mouse brains. Inset within merged image panels C, F, I, L indicate the relative position of the hypothalamic region imaged. Only a representative sample of coexpressing cells from each panel was marked. Scale bar, 50 μm. Corresponding red-green images are provided in Figure 11.
CNE_23273_sm_SuppFig3.tif7242KSupporting Information Figure 3. Characterization of MCHR1-expressing neurons in the lateral hypothalamus and arcuate nucleus of the hypothalamus. Representative photomicrographs of two-color confocal images showing that a subpopulation of tdTomato fluorescent neurons express immunoreactivity for A-C, neurotensin in the ventral subfornical region of the lateral hypothalamus; D-F, phospho-STAT3 in the arcuate nucleus. Singly-labeled neurons appear either magenta or green while coexpression (arrowhead) is indicated by cells appearing white due to color overlay in the merged image (C, F). All stainings were performed in Mchr1-cre/tdTomato mouse brains. Inset within merged image panels C, F indicate the relative position of the hypothalamic region imaged. Only a representative sample of coexpressing cells from each panel was marked. Scale bar, 50 μm. Corresponding magenta-green images are provided in Figure 12.
CNE_23273_sm_SuppFig4.tif13422KSupporting Information Figure 4. Characterization of leptin-sensitive MCHR1-expressing neurons in the arcuate nucleus of the hypothalamus. Photomicrographs of three-color confocal images showing coexpression of arcuate tdTomato fluorescence (magenta; A, E), phoshpo-STAT3 (cyan; B, F) and humanized, renilla green fluorescent protein (hrGFP) (green; C, G) with A-D, Neuropeptide Y neurons from the Npy-hrGFP; Mchr1-cre/tdTomato mouse brain; E-H, proopiomelanocortin neurons from the Pomc-hrGFP; Mchr1-cre/tdTomato mouse brain. Triple labeled neurons (arrowhead) appear white due to color overlay in the merged image (D, H). Scale bar, 50 μm. Corresponding magenta-green-cyan images are provided in Figure 13.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.