Characterization of multiple bistratified retinal ganglion cells in a purkinje cell protein 2-Cre transgenic mouse line

Authors

  • Elena Ivanova,

    1. Department of Ophthalmology and Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan
    Current affiliation:
    1. Burke Medical Research Institute, Weill Medical College of Cornell University, White Plains, NY
    Search for more papers by this author
  • Patrick Lee,

    1. Department of Ophthalmology and Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan
    Search for more papers by this author
  • Zhuo-Hua Pan

    Corresponding author
    1. Department of Ophthalmology and Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan
    • Department of Ophthalmology and Anatomy & Cell Biology, Wayne State University School of Medicine, 540 E. Canfield Ave., Detroit, MI 48201
    Search for more papers by this author

Abstract

Retinal ganglion cells are categorized into multiple classes, including multiple types of bistratified ganglion cells (BGCs). The recent use of transgenic mouse lines with specific type(s) of ganglion cells that are labeled by fluorescent markers has facilitated the morphological and physiological studies of BGCs, particularly the directional-selective BGCs. The most important benefit from using transgenic animals is the capability to perform in vivo gene manipulation. In particular, the Cre/LoxP recombination system has become a powerful tool, allowing gene deletion, overexpression, and ectopic expression in a cell type-specific and temporally controlled fashion. The key to this tool is the availability of Cre mouse lines with cell or tissue type-specific expression of Cre recombinase. In this study we characterized the Cre-positive retinal ganglion cells in a PCP2 (Purkinje cell protein 2)-cre mouse line. We found that all of the Cre-positive retinal ganglion cells were BGCs. Based on morphological criteria, we determined that they can be grouped into five types. The On- and Off-dendrites of three of these types stratified outside of the cholinergic bands and differed from directional selective ganglion cells (DSGCs) morphologically. These cells were negative for Brn-3b and positive for both calretinin and CART retina markers. The remaining two types were identified as putative On-Off and On-DSGCs. This Cre mouse line could be useful for further studies of the molecular and functional properties of BGCs in mice. J. Comp. Neurol. 521:2165–2180, 2013. © 2012 Wiley Periodicals, Inc.

Ancillary