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CNE_23296_sm_SuppInfo.tif16192KSupporting Figure 2. Paired fluorescence images of guinea-pig ileum myenteric ganglia immunohistochemically double-labeled with antisera against α-synuclein and either 5-HT (A & B), calretinin (D & E), VAChT (G & H) or VIP (J & K). Each pair of micrographs was screened to generate an overlay that contained varicosities labeled with both markers (C, F, I and L). Varicosities that contained immunoreactivity for α-synuclein and the other marker are indicated by arrows and varicosities immunoreactive for the other marker alone are indicated by arrowheads. Scale bars = 20 μm. Abbreviations: 5-HT, 5-hydroxytryptamine; α-Syn, α-synuclein; VAChT, vesicular acetylcholine transporter; VIP, vasoactive intestinal polypeptide. A magenta-green version of this figure has been provided as a supplementary file for the assistance of color-blind readers.

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