Additional Supporting Information may be found in the online version of this article.

CNE_23296_sm_SuppFig2.tif16182KSupplementary Figure 1 (magenta-green version of Figure 2): NGF mRNA and protein in the mouse descending colon and urinary bladder. A: NGF mRNA levels in the descending colon and urinary bladder are comparable among NGF/p75+/+ (lanes 1 and 2), NGF/p75-/- (lanes 3 and 4), and WT (lanes 5 and 6) mice. B: Immunostaining for NGF by Western blotting (upper membrane) reveals bands at ∼12, ∼14, and ∼37 kDa in the descending colon of NGF/p75+/+ mice (lane 2), NGF/p75-/- mice (lane 3), and WT mice (lane 4). In contrast, only a single band at ∼14 kDa is detected in the urinary bladder of NGF/p75+/+ mice (lane 5), NGF/p75-/- mice (lane 6), and WT mice (lane 7). Standards for NGF include proNGF (0.5 ng; lane 1 resolved at ∼32kDa), 2.5S NGF (0.5 ng; lane 8 resolved at ∼13 kDa), and the male mouse submaxillary gland (1μg; lane 9 resolved at ∼13 kDa). Immunostaining for β-actin (∼42 kDa in red) by Western blotting (lower membrane) shows equal loading of protein samples in lanes 2-4 for the descending colons and in lanes 5-7 for the urinary bladders. L, ladder. (see Supplemental Figure 1 for images converted to magenta-green).
CNE_23296_sm_SuppFig11.tif16183KSupplementary Figure 2 (magenta-green version of Figure 11): Sympathetic plexus formation around sensory neurons in the dorsal root ganglia. No sympathetic perineuronal plexuses (as detected by TH-immunostaining were seen in the sensory ganglia of wild type mice (A, D, G, J). In NGF mice with a fully functional p75NTR (B, E, H, K), sympathetic plexuses (green) were associated with trkA- (E, red), CGRP- (H, red), and p75NTR- (K, red) immunopositive neurons. In NGF mice carrying a p75NTR null mutant (C, F, I, L), TH-immunopositive sympathetic fibers were found extending throughout the ganglionic environment with very few perineuronal plexuses seen. Small TH-immunopositive neurons (green) were observed in the dorsal root ganglia of all lines of transgenic mice. As an internal control, no p75NTR immunostaining was detected in NGF/p75-/- mice (L). Scale bar = 100 μm. (see Supplemental Figure 2 for images converted to magenta-green).

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.