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CNE_23303_sm_SuppFig1.tif1783KSupplementary Figure 1: (Magenta-green version of Figure 1 for the assistance of color-blind readers.) A. Comparative brain size in three adult (3 months old) muridae: left, Rattus norvegicus (Wistar; 200g bw.); center, Arvicanthis niloticus (64g bw.); right, Mus musculus (C57BL/6J; 20g bw.). The rostrocaudal extent of the cerebral hemispheres is 14.6 mm in rat, 11.3 mm in Nile rat, and 8.2 mm in mouse. B. Optic nerves and tracts of Arvicanthis niloticus filled with fluorescently-tagged CTB. Case NR155, horizontal plane, 30 μm beneath the SCN. The rostrocaudal extent of the optic chiasm is ≈1.3 mm. C. Arrangement of the ipsilateral and contralateral optic tract fibers at the chiasma level. Case NR152, sagittal plane, 180 μm lateral from midline. Note the predominance of ipsilateral afferents in the rostral part of the SCN (open arrow). C, caudal; D, dorsal; R, rostral; ipsi, ipsilateral tract; contra, contralateral tract.
CNE_23303_sm_SuppFig2.tif7474KSupplementary Figure 2: (Magenta-green version of Figure 3 for the assistance of color-blind readers.) Retinal projections to the geniculate complex. A. Contralateral projections as viewed at the mid rostrocaudal level (810 μm from the frontal pole). Giemsa counterstaining, coronal plane, case NR8. B. Enlarged view of the dLGN showing the pseudo-striated projection pattern due to fiber bundles crossing the dLGN parallel to the optic tract. C. Ipsilateral projection pattern at the same rostrocaudal level as above. Coronal plane. D. Enlargement of the inset in C showing different types of terminal patterns in the dLGN. E. Dual retinal projections in the dLGN following bilateral ocular injection of fluorescently-tagged CTB. Case NR152, sagittal plane, right side of the brain, 720 μm from the OT surface. Contralateral input is green; ipsilateral input is magenta. Open arrows point to scant overlapping bilateral projections. F. ‘Tail’ of the IGL located between the median geniculate nucleus and zona incerta. Case NR8, Giemsa-stained section, coronal plane, ≈1200 μm from the frontal pole of the dLGN. G. Rostral expansion of the IGL following bilateral ocular injection of fluorescently-tagged CTB. Case NR152, sagittal plane, right side of the brain, 360 μm from the OT surface. Contralateral (green) and ipsilateral (magenta) inputs are interspersed along the IGL, including the dorsal cap (open arrow). C, caudal; D, dorsal.
CNE_23303_sm_SuppFig3.tif2606KSupplementary Figure 3: (Magenta-green version of Figure 4 for the assistance of color-blind readers.) Ipsilateral inputs to the geniculate complex. A. Projection pattern in the internal subdivision of the vLGN-mc as viewed on three consecutive sagittal sections (the most lateral is left, ≈180 μm from the lateral border of the OT) following bilateral ocular injection of fluorescently-tagged CTB. Case NR152, left side of the brain. Ipsilateral and contralateral inputs are green and magenta, respectively. B. Same pattern as in panel A but viewed on a horizontal plane at the IGL/vLGN interface. Case NR155, right side of the brain (rostral up). Ipsilateral and contralateral inputs are red and green, respectively. Note in both panels A and B, the presence of ipsilateral input in the internal (i), but not the external (e) sector of the vLGN-mc. The parvocellular (pc) division of the vLGN is deprived of fluorescent staining. C, D. Enlargement of loci (arrows in A) suspected to receive overlapping bilateral inputs. E. Reconstruction of the ipsilaterally derived retinal projection to the whole geniculate complex as viewed from successive (90 μm apart) Giemsa-stained sections. Case NR8, coronal plane, right side of the brain. The overall ipsilaterally derived retinal projection to the vLGN-mc is slightly switched caudally as compared with its counterpart in the dLGN. Patches of dense ipsilateral terminals (black areas) represent ≈4.85% of the dLGN volume. Greyish areas contain only sparse isolated labeled fibers. C, caudal; D, dorsal.
CNE_23303_sm_SuppFig4.tif3700KSupplementary Figure 4: (Magenta-green version of Figure 8 for the assistance of color-blind readers.) Organization of the pretectum. A. Location of the pretectal nuclei along the rostrocaudal axis. Semi-schematic drawings from CTB-reacted, Giemsa-stained coronal sections (case NR8). Nuclei are identified in each section by the following symbols: (▴) APTD, (♦) MPT, (•) OPT, (▪) PPT, (▾) NOT and () PLi. Dots in each nucleus refer to the spatial distribution and the density of the ipsilaterally derived projections. The first section (bottom) is 450 μm caudal to the anterior pole of the dLGN. B-E. Contralateral (green) and ipsilateral (magenta) projections in four consecutive horizontal sections (case NR155) set above (+180 μm, B; +90 μm, C) and below (-90 μm, E) the reference level (dorsal surface of the dLGN in D). Double fluorescent material. Magenta and green levels have been adjusted equally. Scale bar in E applies to all microphotographs. Stars indicate the PLi nucleus. Large green and white spots (in C and E) are staining artifacts. In both columns, the median axis of the brain is to the left (narrow white vertical line on extreme left of each panel). LAT, lateral; R, rostral.
CNE_23303_sm_SuppFig5.tif3775KSupplementary Figure 5: (Magenta-green version of Figure 10 for the assistance of color-blind readers.) Morphology of the SC. A. Nissl-stained coronal section (case NR8) at the mid rostrocaudal level. The lateral extent of the right SC is 2310 μm; and the full length of the dorsal surface is 4050 μm. B. Nissl-stained sagittal section (case NR9; 900 μm from the midline) showing the characteristic layering of the SC. C. The SGS-SO boundary respectively defined in horizontal (left; 1260 μm beneath the dorsal surface of the SC; case NR155) and sagittal (right; 630 μm from midline; case NR152) planes from fluorescently-labeled material. Arrows point to patches of terminals from the uncrossed projection. Magenta, input from the right eye; Green, input from the left eye. D. Left: Total dorsal surface of the SC of A. niloticus (black dots) compared with that of a Wistar rat (circles) and of a mouse (black squares). Values for A. niloticus correspond to the full length of the dorsal outer limit of SC as measured from consecutive Giemsa-stained coronal sections. Values for rat and mouse are derived from atlas drawings. Right: Topography of the ipsilateral retinotectal projections in A. niloticus. The median grayish area shows the extent of the densest diffuse ipsilateral projection. Black lines at the rostrolateral pole of the SC correspond to the location of dense patches of ipsilateral terminals in SO. E. Volumes of the SC, visual layers and retinotectal projections in mouse (black columns), rat (greyish columns) and Nile grass rat (white columns) normalized against the corresponding brain volumes. C, caudal; D, dorsal; LAT, lateral; Retina proj. in E refres to the projection from the contralateral retina.
CNE_23303_sm_SuppFig6.tif603KSupplementary Figure 6: (Magenta-green version of Figure 13 for the assistance of color-blind readers.) Retrograde staining in the right oculomotor nucleus (n3) following intravitreal injections of Alexa594-tagged CTB (magenta) in the right eye and Alexa488-tagged CTB (green) in the left eye. Case NR152, sagittal section at the mid brain level. Contralaterally and ipsilaterally projecting neurons occupy the caudal (left) and rostral (right) part of the nucleus, respectively. There is no retrograde labeling outside the n3 nucleus in this section.

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