Maturation of peptide-positive synaptic arbors in the medicinal leech requires rhythmic target activity
Article first published online: 19 JUN 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Comparative Neurology
Volume 521, Issue 12, pages 2833–2849, 15 August 2013
How to Cite
Kueh, D., Appiah, J. and Jellies, J. (2013), Maturation of peptide-positive synaptic arbors in the medicinal leech requires rhythmic target activity. J. Comp. Neurol., 521: 2833–2849. doi: 10.1002/cne.23319
- Issue published online: 19 JUN 2013
- Article first published online: 19 JUN 2013
- Accepted manuscript online: 22 FEB 2013 07:35AM EST
- Manuscript Accepted: 5 FEB 2013
- Manuscript Revised: 22 DEC 2012
- Manuscript Received: 22 JUN 2012
- National Science Foundation. Grant Numbers: 9724064, DBI-1062883
- Faculty Research and Creative Activities Award (WMU; to J.J.)
Additional Supporting Information may be found in the online version of this article.
|cne23319-sup-0001-suppfig1.tif||8876K||Supplementary Figure 1 (This is a magenta-green version of Figure 2 from the manuscript for the assistance of color-blind readers.): The mature pattern of innervation on a partial segment of a leech heart tube. A: The mature presynaptic axons labeled by anti-ACT (green), is characterized by complex branching and extensive peptide-positive varicosities labeled by an anti-FMRFamide (red) label (presented here as magenta). FMRFamide appears to be preferentially located in varicosities devoid of microtubules. The arrow points to a main nerve that is projected by an HE neuron. Since anti-ACT labels all axons, many non-HE processes can be seen coursing through the body at different focal planes. The outline of the heart tube and side vessels (LaV and LdV) is indicated by dotted lines. B: Channels of labeled axons and varicosities that were independently traced and merged. The resultant pixel numbers were used to calculate an arbor index (R/ R+G+Y), which allowed us to estimate the total proportion of arbor length that is FMRF+ and ACT- in defined regions. Anterior is up and scale bar applies to both panels. (A magenta-green version of this figure has been posted as a supplementary file for the assistance of color-blind readers.)|
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